Slap (SLA) (NM_001282965) Human Tagged ORF Clone

CAT#: RG236220

SLA (tGFP-tagged) - Human Src-like-adaptor (SLA), transcript variant 5

Product Images

Circular map for RG236220

Specifications

Product Data

• Tag: TurboGFP
• Symbol: Slap
• Synonyms: SLA1; SLAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236220 representing NM_001282965.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATCAGAGAGAGTGAGACCAAGAAAGGGTTTTACTCACTGTCGGTGAGACACAGGCAGGTAAAGCAT
  TACCGCATTTTCCGTCTGCCCAACAACTGGTACTACATTTCCCCGAGGCTCACCTTCCAGTGCCTGGAG
  GACCTGGTGAACCACTATTCTGAGGTGGCTGATGGCCTGTGCTGTGTGCTCACCACGCCCTGCCTGACA
  CAAAGCACGGCTGCCCCAGCAGTGAGGGCCTCCAGCTCACCTGTCACCTTGCGTCAGAAGACTGTGGAC
  TGGAGGAGAGTGTCCAGACTGCAGGAGGACCCCGAGGGAACAGAGAACCCGCTTGGGGTAGACGAGTCC
  CTTTTCAGCTATGGCCTTCGAGAGAGCATTGCCTCTTACCTGTCCCTGACCAGTGAGGACAACACCTCC
  TTTGATCGAAAGAAGAAAAGCATCTCCCTGATGTATGGTGGCAGCAAGAGAAAGAGCTCATTCTTCTCA
  TCACCACCTTACTTTGAGGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236220
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MIRESETKKGFYSLSVRHRQVKHYRIFRLPNNWYYISPRLTFQCLEDLVNHYSEVADGLCCVLTTPCLT
  QSTAAPAVRASSSPVTLRQKTVDWRRVSRLQEDPEGTENPLGVDESLFSYGLRESIASYLSLTSEDNTS
  FDRKKKSISLMYGGSKRKSSFFSSPPYFED
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282965
• ORF Size: 504 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001282965.2
• RefSeq Size: 3096 bp
• RefSeq ORF: 507 bp
• Locus ID: 6503
• UniProt ID: Q13239
• Cytogenetics: 8q24.22
• Protein Families: Druggable Genome
• MW: 19.7 kDa
• Gene Summary: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.[UniProtKB/Swiss-Prot Function]