MT1F (NM_001301272) Human Tagged ORF Clone

CAT#: RG235417

MT1F (tGFP-tagged) - Human metallothionein 1F (MT1F), transcript variant 2

Product Images

Circular map for RG235417

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MT1F
• Synonyms: MT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235417 representing NM_001301272.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACCCCAACTGCTCCTGCGCCGCTGGTGTCTCCTGCACCTGCGCTGGTTCCTGCAAGTGCAAAGAG
  TGCAAATGCACCTCCTGCAAGAAGAGTGAGTGTGAGGCCATCTCCATGGTCTGGGGCTGTGGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG235417
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDPNCSCAAGVSCTCAGSCKCKECKCTSCKKSECEAISMVWGCG
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301272
• ORF Size: 132 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001301272.1, NP_001288201.1
• RefSeq Size: 498 bp
• RefSeq ORF: 135 bp
• Locus ID: 4494
• UniProt ID: P04733
• Cytogenetics: 16q13
• MW: 5 kDa
• Gene Summary: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.[UniProtKB/Swiss-Prot Function]