ARID5B (NM_001244638) Human Tagged ORF Clone

CAT#: RG235069

ARID5B (tGFP-tagged) - Homo sapiens AT rich interactive domain 5B (MRF1-like) (ARID5B), transcript variant 2

Product Images

Circular map for RG235069

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARID5B
• Synonyms: DESRT; MRF-2; MRF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235069 representing NM_001244638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCAAATCTTAAAGGCAGACCACGCAAAAAGAAACCATGCCCACAAAGAAGAGATTCATTCAGTG
  GTGTTAAGGATTCCAACAACAATTCCGATGGCAAAGCCGTTGCCAAGGTGAAATGTGAGGCCAGGTCAGC
  CTTGACCAAGCCGAAGAATAACCATAACTGTAAAAAAGTCTCAAATGAAGAAAAACCAAAGGTTGCCATT
  GGTGAAGAGTGCAGGGCAGATGAACAAGCCTTCTTGGTGGCACTTTATAAATACATGAAAGAAAGGAAAA
  CGCCGATAGAACGAATACCCTATTTAGGTTTTAAACAGATTAACCTTTGGACTATGTTTCAAGCTGCTCA
  AAAACTGGGAGGATATGAAACAATAACAGCCCGCCGTCAGTGGAAACATATTTATGATGAATTAGGCGGT
  AATCCTGGGAGCACCAGCGCTGCCACTTGTACCCGCAGACATTATGAAAGATTAATCCTACCATATGAAA
  GATTTATTAAAGGAGAAGAAGATAAGCCCCTGCCTCCAATCAAACCTCGGAAACAGGAGAACAGTTCACA
  GGAAAATGAGAACAAAACAAAAGTATCTGGAACCAAACGCATCAAACATGAAATACCTAAAAGCAAGAAA
  GAAAAAGAAAATGCCCCAAAGCCCCAGGATGCAGCAGAGGTTTCATCAGAGCAAGAAAAAGAACAAGAGA
  CTTTAATAAGCCAGAAAAGCATCCCTGAGCCTCTCCCAGCAGCAGACATGAAGAAAAAAATAGAAGGGTA
  TCAGGAATTTTCAGCGAAGCCCCTGGCATCCAGAGTAGACCCAGAGAAGGACAACGAAACAGACCAAGGT
  TCCAACAGTGAGAAGGTGGCAGAGGAGGCGGGAGAGAAGGGGCCCACACCTCCACTCCCAAGTGCTCCTC
  TGGCCCCAGAAAAAGATTCAGCCTTGGTCCCTGGGGCCAGCAAACAGCCACTCACCTCTCCTAGTGCCCT
  GGTGGACTCAAAACAAGAATCCAAACTGTGCTGTTTTACAGAGAGCCCTGAAAGTGAACCCCAAGAAGCA
  TCCTTCCCCAGCTTCCCCACCACACAGCCACCGCTGGCAAACCAGAATGAGACGGAGGATGACAAACTGC
  CCGCCATGGCAGATTACATTGCCAACTGCACCGTGAAGGTGGACCAGCTGGGCAGTGACGACATCCACAA
  TGCGCTCAAGCAGACCCCAAAGGTCCTTGTGGTCCAGTCGTTTGACATGTTCAAAGACAAAGACCTGACT
  GGGCCCATGAACGAGAACCATGGACTTAATTACACGCCCCTGCTCTACTCTAGGGGCAACCCAGGCATCA
  TGTCCCCACTGGCCAAGAAAAAGCTTTTGTCCCAAGTGAGTGGGGCCAGCCTCTCCAGCAGCTACCCTTA
  TGGCTCCCCACCCCCTTTGATCAGCAAAAAGAAACTGATTGCTAGGGATGACTTGTGTTCCAGTTTGTCC
  CAGACCCACCATGGCCAAAGCACTGACCATATGGCGGTCAGCCGGCCATCAGTGATTCAGCACGTCCAGA
  GTTTCAGAAGCAAGCCCTCGGAAGAGAGAAAGACCATCAATGACATCTTTAAGCATGAGAAACTGAGTCG
  ATCAGATCCCCACCGCTGCAGCTTCTCCAAGCATCACCTTAACCCCCTTGCTGACTCCTACGTCCTGAAG
  CAAGAAATTCAGGAGGGCAAGGATAAACTCTTAGAGAAAAGGGCCCTCCCCCATTCCCACATGCCTAGCT
  TCCTGGCTGACTTCTACTCGTCCCCTCATCTCCATAGCCTCTACAGACACACCGAGCACCATCTTCATAA
  TGAACAGACATCCAAATACCCTTCCAGGGACATGTACAGGGAATCGGAAAACAGTTCTTTTCCTTCCCAC
  AGACACCAAGAAAAGCTCCATGTAAATTATCTCACGTCCCTGCACCTGCAAGACAAAAAGTCGGCGGCAG
  CAGAAGCCCCTACGGATGATCAGCCTACAGATCTGAGCCTTCCCAAGAACCCGCACAAACCTACCGGCAA
  GGTCCTGGGCCTGGCTCATTCCACCACAGGGCCCCAGGAGAGCAAAGGCATCTCCCAGTTCCAGGTCTTA
  GGCAGCCAGAGTCGAGACTGTCACCCCAAAGCCTGTCGGGTATCACCCATGACCATGTCAGGCCCTAAAA
  AATACCCTGAATCGCTTTCAAGATCAGGAAAACCTCACCATGTGAGACTGGAGAATTTCAGGAAGATGGA
  AGGCATGGTCCACCCAATCCTGCACCGGAAAATGAGCCCGCAGAACATTGGGGCGGCGCGGCCGATCAAG
  CGCAGCCTGGAGGATTTGGACCTTGTGATTGCAGGGAAAAAGGCCCGGGCAGTGTCTCCCTTAGACCCAT
  CCAAGGAGGTCTCTGGGAAGGAGAAGGCCTCTGAGCAGGAGAGTGAAGGCAGCAAAGCAGCGCACGGTGG
  GCATTCCGGGGGCGGATCAGAAGGCCACAAGCTTCCCCTCTCCTCCCCTATCTTCCCAGGTCTGTATTCC
  GGGAGCCTGTGTAACTCGGGCCTCAACTCCAGGCTCCCGGCTGGGTATTCTCATTCTCTGCAGTACTTGA
  AAAACCAGACTGTGCTTTCTCCACTCATGCAGCCCCTGGCTTTCCACTCGCTTGTGATGCAAAGAGGAAT
  TTTTACATCACCGACAAATTCTCAGCAGCTGTACAGACACTTGGCTGCGGCTACACCTGTAGGAAGTTCA
  TATGGGGACCTTTTGCATAACAGCATTTACCCTTTAGCTGCTATAAATCCTCAAGCTGCCTTTCCATCTT
  CCCAGCTGTCATCCGTGCACCCCAGTACAAAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235069 representing NM_001244638
  Red=Cloning site Green=Tags(s)
  
  MAPNLKGRPRKKKPCPQRRDSFSGVKDSNNNSDGKAVAKVKCEARSALTKPKNNHNCKKVSNEEKPKVAI
  GEECRADEQAFLVALYKYMKERKTPIERIPYLGFKQINLWTMFQAAQKLGGYETITARRQWKHIYDELGG
  NPGSTSAATCTRRHYERLILPYERFIKGEEDKPLPPIKPRKQENSSQENENKTKVSGTKRIKHEIPKSKK
  EKENAPKPQDAAEVSSEQEKEQETLISQKSIPEPLPAADMKKKIEGYQEFSAKPLASRVDPEKDNETDQG
  SNSEKVAEEAGEKGPTPPLPSAPLAPEKDSALVPGASKQPLTSPSALVDSKQESKLCCFTESPESEPQEA
  SFPSFPTTQPPLANQNETEDDKLPAMADYIANCTVKVDQLGSDDIHNALKQTPKVLVVQSFDMFKDKDLT
  GPMNENHGLNYTPLLYSRGNPGIMSPLAKKKLLSQVSGASLSSSYPYGSPPPLISKKKLIARDDLCSSLS
  QTHHGQSTDHMAVSRPSVIQHVQSFRSKPSEERKTINDIFKHEKLSRSDPHRCSFSKHHLNPLADSYVLK
  QEIQEGKDKLLEKRALPHSHMPSFLADFYSSPHLHSLYRHTEHHLHNEQTSKYPSRDMYRESENSSFPSH
  RHQEKLHVNYLTSLHLQDKKSAAAEAPTDDQPTDLSLPKNPHKPTGKVLGLAHSTTGPQESKGISQFQVL
  GSQSRDCHPKACRVSPMTMSGPKKYPESLSRSGKPHHVRLENFRKMEGMVHPILHRKMSPQNIGAARPIK
  RSLEDLDLVIAGKKARAVSPLDPSKEVSGKEKASEQESEGSKAAHGGHSGGGSEGHKLPLSSPIFPGLYS
  GSLCNSGLNSRLPAGYSHSLQYLKNQTVLSPLMQPLAFHSLVMQRGIFTSPTNSQQLYRHLAAATPVGSS
  YGDLLHNSIYPLAAINPQAAFPSSQLSSVHPSTKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001244638
• ORF Size: 2835 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244638.2
• RefSeq Size: 7032 bp
• RefSeq ORF: 2838 bp
• Locus ID: 84159
• UniProt ID: Q14865
• Cytogenetics: 10q21.2
• Gene Summary: This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]