PDE2A (NM_001243784) Human Tagged ORF Clone

CAT#: RG235036

PDE2A (tGFP-tagged) - Homo sapiens phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 3

Product Images

Circular map for RG235036

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE2A
• Synonyms: CGS-PDE; cGSPDE; PDE2A1; PED2A4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235036 representing NM_001243784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCGACAGCCTGCAGCCAGCCTGGACCCCCTTGCAAAGGAGCCAGGACCCCCAGGGAGTAGAGACG
  ACCGACTGGAGGACGCCTTGCTGAGTCTGGGCTCTGTCATCGACATTTCAGGCCTGCAACGTGCTGTCAA
  GGAGGCCCTGTCAGCTGTGCTCCCCCGAGTGGAAACTGTCTACACCTACCTACTGGATGGTGAGTCCCAG
  CTGGTGTGTGAGGACCCCCCACATGAGCTGCCCCAGGAGGGGAAAGTCCGGGAGGCTATCATCTCCCAGA
  AGCGGCTGGGCTGCAATGGGCTGGGCTTCTCAGACCTGCCAGGGAAGCCCTTGGCCAGGCTGGTGGCTCC
  ACTGGCTCCTGATACCCAAGTGCTGGTCATGCCGCTAGCGGACAAGGAGGCTGGGGCCGTGGCAGCTGTC
  ATCTTGGTGCACTGTGGCCAGCTGAGTGATAATGAGGAATGGAGCCTGCAGGCGGTGGAGAAGCATACCC
  TGGTCGCCCTGCGGAGGGTGCAGGTCCTGCAGCAGCGCGGGCCCAGGGAGGCTCCCCGAGCCGTCCAGAA
  CCCCCCGGAGGGGACGGCGGAAGACCAGAAGGGCGGGGCGGCGTACACCGACCGCGACCGCAAGATCCTC
  CAACTGTGCGGGGAACTCTACGACCTGGATGCCTCTTCCCTGCAGCTCAAAGTGCTCCAATACCTGCAGC
  AGGAGACCCGGGCATCCCGCTGCTGCCTCCTGCTGGTGTCGGAGGACAATCTCCAGCTTTCTTGCAAGGT
  CATCGGAGACAAAGTGCTCGGGGAAGAGGTCAGCTTTCCCTTGACAGGATGCCTGGGCCAGGTGGTGGAA
  GACAAGAAGTCCATCCAGCTGAAGGACCTCACCTCCGAGGATGTACAACAGCTGCAGAGCATGTTGGGCT
  GTGAGCTGCAGGCCATGCTCTGTGTCCCTGTCATCAGCCGGGCCACTGACCAGGTGGTGGCCTTGGCCTG
  CGCCTTCAACAAGCTAGAAGGAGACTTGTTCACCGACGAGGACGAGCATGTGATCCAGCACTGCTTCCAC
  TACACCAGCACCGTGCTCACCAGCACCCTGGCCTTCCAGAAGGAACAGAAACTCAAGTGTGAGTGCCAGG
  CTCTTCTCCAAGTGGCAAAGAACCTCTTCACCCACCTGGATGACGTCTCTGTCCTGCTCCAGGAGATCAT
  CACGGAGGCCAGAAACCTCAGCAACGCAGAGATCTGCTCTGTGTTCCTGCTGGATCAGAATGAGCTGGTG
  GCCAAGGTGTTCGACGGGGGCGTGGTGGATGATGAGAGCTATGAGATCCGCATCCCGGCCGATCAGGGCA
  TCGCGGGACACGTGGCGACCACGGGCCAGATCCTGAACATCCCTGACGCATATGCCCATCCGCTTTTCTA
  CCGCGGCGTGGACGACAGCACCGGCTTCCGCACGCGCAACATCCTCTGCTTCCCCATCAAGAACGAGAAC
  CAGGAGGTCATCGGTGTGGCCGAGCTGGTGAACAAGATCAATGGGCCATGGTTCAGCAAGTTCGACGAGG
  ACCTGGCGACGGCCTTCTCCATCTACTGCGGCATCAGCATCGCCCATTCTCTCCTATACAAAAAAGTGAA
  TGAGGCTCAGTATCGCAGCCACCTGGCCAATGAGATGATGATGTACCACATGAAGGTCTCCGATGATGAG
  TATACCAAACTTCTCCATGATGGGATCCAGCCTGTGGCTGCCATTGACTCCAATTTTGCAAGTTTCACCT
  ATACCCCTCGTTCCCTGCCCGAGGATGACACGTCCATGGCCATCCTGAGCATGCTGCAGGACATGAATTT
  CATCAACAACTACAAAATTGACTGCCCGACCCTGGCCCGGTTCTGTTTGATGGTGAAGAAGGGCTACCGG
  GATCCCCCCTACCACAACTGGATGCACGCCTTTTCTGTCTCCCACTTCTGCTACCTGCTCTACAAGAACC
  TGGAGCTCACCAACTACCTCGAGGACATCGAGATCTTTGCCTTGTTTATTTCCTGCATGTGTCATGACCT
  GGACCACAGAGGCACAAACAACTCTTTCCAGGTGGCCTCGAAATCTGTGCTGGCTGCGCTCTACAGCTCT
  GAGGGCTCCGTCATGGAGAGGCACCACTTTGCTCAGGCCATCGCCATCCTCAACACCCACGGCTGCAACA
  TCTTTGATCATTTCTCCCGGAAGGACTATCAGCGCATGCTGGATCTGATGCGGGACATCATCTTGGCCAC
  AGACCTGGCCCACCATCTCCGCATCTTCAAGGACCTCCAGAAGATGGCTGAGGTGGGCTACGACCGAAAC
  AACAAGCAGCACCACAGACTTCTCCTCTGCCTCCTCATGACCTCCTGTGACCTCTCTGACCAGACCAAGG
  GCTGGAAGACTACGAGAAAGATCGCGGAGCTGATCTACAAAGAATTCTTCTCCCAGGGAGACCTGGAGAA
  GGCCATGGGCAACAGGCCGATGGAGATGATGGACCGGGAGAAGGCCTATATCCCTGAGCTGCAAATCAGC
  TTCATGGAGCACATTGCAATGCCCATCTACAAGCTGTTGCAGGACCTGTTCCCCAAAGCGGCAGAGCTGT
  ACGAGCGCGTGGCCTCCAACCGTGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGCGGCCTCCC
  AAGTAACAACTCGCTGGACTTCCTGGATGAGGAGTACGAGGTGCCTGATCTGGATGGCACTAGGGCCCCC
  ATCAATGGCTGCTGCAGCCTTGATGCTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235036 representing NM_001243784
  Red=Cloning site Green=Tags(s)
  
  MRRQPAASLDPLAKEPGPPGSRDDRLEDALLSLGSVIDISGLQRAVKEALSAVLPRVETVYTYLLDGESQ
  LVCEDPPHELPQEGKVREAIISQKRLGCNGLGFSDLPGKPLARLVAPLAPDTQVLVMPLADKEAGAVAAV
  ILVHCGQLSDNEEWSLQAVEKHTLVALRRVQVLQQRGPREAPRAVQNPPEGTAEDQKGGAAYTDRDRKIL
  QLCGELYDLDASSLQLKVLQYLQQETRASRCCLLLVSEDNLQLSCKVIGDKVLGEEVSFPLTGCLGQVVE
  DKKSIQLKDLTSEDVQQLQSMLGCELQAMLCVPVISRATDQVVALACAFNKLEGDLFTDEDEHVIQHCFH
  YTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELV
  AKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNEN
  QEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDE
  YTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYR
  DPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSS
  EGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRN
  NKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQIS
  FMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDGTRAP
  INGCCSLDAE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243784
• ORF Size: 2760 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243784.1, NP_001230713.1
• RefSeq Size: 4208 bp
• RefSeq ORF: 2763 bp
• Locus ID: 5138
• UniProt ID: O00408
• Cytogenetics: 11q13.4
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line) (PubMed:24705027).[UniProtKB/Swiss-Prot Function]