SAFB (NM_001201339) Human Tagged ORF Clone

CAT#: RG235029

SAFB (tGFP-tagged) - Homo sapiens scaffold attachment factor B (SAFB), transcript variant 2

Product Images

Circular map for RG235029

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SAFB
• Synonyms: HAP; HET; SAB-B1; SAF-B; SAF-B1; SAFB1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235029 representing NM_001201339
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACTCTGTCAGGCCTAGGTGATTCTGGAGCGGCGGGCGCGGCGGCTCTGAGCTCCGCCTCGT
  CAGAGACCGGGACGCGGCGCCTCAGCGACCTGCGAGTGATCGATCTGCGGGCGGAGCTGAGGAAACGGAA
  TGTGGACTCGAGCGGCAACAAGAGCGTTTTGATGGAGCGGCTGAAGAAGGCAATTGAAGATGAAGGTGGT
  AATCCTGACGAAATTGAAATTACCTCCGAGGGAAACAAGAAAACATCAAAGAGGTCTAGCAAAGGGCGCA
  AACCAGAAGAAGAGGGTGTGGAAGATAACGGGCTGGAGGAAAACTCTGGGGATGGACAGGAGGATGTTGA
  GACCAGTCTGGAGAACTTGCAGGACATCGACATCATGGATATCAGTGTGTTGGATGAAGCAGAAATTGAT
  AATGGAAGCGTTGCAGATTGTGTCGAAGACGATGATGCTGATAACCTCCAGGAGTCCCTGTCGGATAGTA
  GAGAGCTAGTCGAGGGGGAAATGAAAGAGCTTCCGGAGCAGCTTCAGGAACATGCTATAGAGGACAAAGA
  AACTATAAACAATTTAGATACTTCATCATCTGACTTCACTATATTACAGGAAATTGAAGAGCCATCCCTG
  GAGCCAGAAAATGAGAAAATACTCGACATTTTGGGGGAAACTTGTAAATCTGAGCCAGTAAAAGAAGAAA
  GTTCCGAGCTGGAGCAGCCATTTGCACAGGACACAAGTAGCGTGGGGCCAGACAGAAAGCTTGCGGAGGA
  AGAGGACCTATTTGACAGCGCCCATCCGGAAGAGGGTGATTTAGATTTGGCCAGCGAGTCAACAGCACAC
  GCTCAGTCGAGCAAGGCAGACAGCCTGTTAGCGGTAGTGAAAAGGGAGCCCGCGGAGCAGCCAGGCGATG
  GCGAGAGGACGGACTGTGAGCCTGTAGGGCTAGAGCCGGCAGTTGAGCAGAGTAGTGCGGCCTCCGAGCT
  CGCGGAGGCCTCTAGCGAGGAGCTCGCAGAAGCACCCACGGAAGCCCCAAGCCCAGAAGCCAGAGATAGC
  AAAGAAGACGGGAGGAAGTTTGATTTTGACGCTTGTAATGAAGTCCCTCCGGCTCCTAAAGAGTCCTCAA
  CCAGTGAGGGCGCTGATCAGAAAATGAGTTCTCCCGAAGATGACTCGGATACAAAAAGGCTTTCCAAAGA
  GGAAAAGGGTCGCAGCAGTTGTGGTAGAAATTTCTGGGTTAGTGGACTCTCTTCTACAACCAGAGCTACA
  GATTTGAAGAATCTTTTCAGCAAATATGGGAAGGTGGTGGGCGCCAAGGTTGTGACAAATGCCCGGAGTC
  CTGGAGCTCGCTGTTACGGTTTTGTCACGATGTCCACAGCAGAAGAGGCCACAAAATGCATTAACCACCT
  GCACAAGACGGAGCTCCACGGAAAGATGATCTCCGTGGAGAAAGCCAAAAATGAACCTGTGGGAAAGAAA
  ACCTCTGACAAAAGAGACAGTGACGGGAAAAAGGAGAAGTCGAGCAACAGTGACAGATCTACAAACCTTA
  AGAGGGATGATAAATGTGACAGAAAAGATGATGCTAAGAAGGGTGACGACGGAAGTGGAGAAAAGAGTAA
  GGACCAAGATGATCAGAAACCTGGCCCCTCAGAGCGATCTCGAGCCACAAAGTCAGGAAGTCGAGGGACC
  GAACGGACTGTAGTAATGGATAAATCCAAAGGGGTGCCTGTGATTAGTGTAAAAACGTCCGGGTCCAAAG
  AGAGAGCTTCCAAAAGCCAGGATCGCAAATCAGCCAGCAGAGAGAAGCGGTCCGTCGTGTCCTTTGATAA
  GGTCAAGGAGCCTCGGAAGTCAAGAGACTCAGAGTCCCATAGGGTGCGTGAACGCAGTGAACGCGAACAA
  CGCATGCAGGCGCAGTGGGAGCGCGAGGAGCGTGAGCGGCTGGAGATTGCCCGAGAGAGGCTGGCCTTCC
  AGCGCCAGCGGCTGGAGCGGGAGCGCATGGAGCGGGAACGGCTGGAGCGCGAACGCATGCACGTGGAGCA
  CGAGCGCAGGCGCGAGCAGGAGCGCATCCACCGTGAGCGCGAGGAGCTGAGGCGCCAGCAGGAACTGCGC
  TATGAGCAGGAGCGGCGGCCCGCGGTGCGGCGGCCCTACGACCTGGACCGGCGAGATGATGCCTATTGGC
  CGGAAGCCAAGCGGGCCGCCCTGGATGAGCGCTACCATTCTGACTTTAACCGCCAGGACCGCTTCCACGA
  CTTTGACCACAGGGACCGCGGCCGCTACCCCGACCACTCGGTGGACAGGAGAGAAGGTTCAAGGTCAATG
  ATGGGAGAACGAGAAGGACAGCATTACCCAGAACGCCATGGAGGACCAGAGCGCCACGGCCGGGACTCCC
  GCGATGGCTGGGGGGGCTATGGCTCTGACAAGAGGATGAGCGAGGGCCGGGGGCTGCCTCCTCCCCCCAG
  GGGCAGACGTGACTGGGGGGACCATGGCCGAAGAGAGGATGACCGGTCATGGCAGGGCACGGCCGACGGG
  GGCATGATGGACAGGGATCACAAGAGGTGGCAAGGTGGCGAGAGAAGCATGTCCGGTCACTCCGGGCCTG
  GCCACATGATGAACCGAGGAGGAATGTCAGGGCGCGGCAGCTTTGCCCCAGGCGGGGCCTCCCGGGGCCA
  CCCCATCCCACACGGTGGCATGCAGGGCGGGTTTGGAGGCCAGAGCCGGGGGAGCAGGCCCAGCGATGCC
  CGCTTCACTCGCCGCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235029 representing NM_001201339
  Red=Cloning site Green=Tags(s)
  
  MAETLSGLGDSGAAGAAALSSASSETGTRRLSDLRVIDLRAELRKRNVDSSGNKSVLMERLKKAIEDEGG
  NPDEIEITSEGNKKTSKRSSKGRKPEEEGVEDNGLEENSGDGQEDVETSLENLQDIDIMDISVLDEAEID
  NGSVADCVEDDDADNLQESLSDSRELVEGEMKELPEQLQEHAIEDKETINNLDTSSSDFTILQEIEEPSL
  EPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAH
  AQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDS
  KEDGRKFDFDACNEVPPAPKESSTSEGADQKMSSPEDDSDTKRLSKEEKGRSSCGRNFWVSGLSSTTRAT
  DLKNLFSKYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAKNEPVGKK
  TSDKRDSDGKKEKSSNSDRSTNLKRDDKCDRKDDAKKGDDGSGEKSKDQDDQKPGPSERSRATKSGSRGT
  ERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKEPRKSRDSESHRVRERSEREQ
  RMQAQWEREERERLEIARERLAFQRQRLERERMERERLERERMHVEHERRREQERIHREREELRRQQELR
  YEQERRPAVRRPYDLDRRDDAYWPEAKRAALDERYHSDFNRQDRFHDFDHRDRGRYPDHSVDRREGSRSM
  MGEREGQHYPERHGGPERHGRDSRDGWGGYGSDKRMSEGRGLPPPPRGRRDWGDHGRREDDRSWQGTADG
  GMMDRDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHPIPHGGMQGGFGGQSRGSRPSDA
  RFTRRY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001201339
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001201339.2
• RefSeq Size: 3118 bp
• RefSeq ORF: 2751 bp
• Locus ID: 6294
• UniProt ID: Q15424
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]