UGT2A1 (NM_001252274) Human Tagged ORF Clone

CAT#: RG234730

UGT2A1 (tGFP-tagged) - Homo sapiens UDP glucuronosyltransferase 2 family, polypeptide A1, complex locus (UGT2A1), transcript variant 2

Product Images

Circular map for RG234730

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UGT2A1
• Synonyms: UDPGT2A1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234730 representing NM_001252274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAAACAACCTTCTGCTGTTCTCCCTTCAGATAAGTCTCATAGGAACCACTCTTGGTGGGAATGTTT
  TGATTTGGCCAATGGAAGGTAGTCATTGGCTAAATGTTAAGATAATTATAGATGAGCTCATTAAAAAGGA
  GCATAATGTGACTGTCCTAGTTGCCTCTGGTGCACTTTTCATCACACCAACCTCTAACCCATCTCTGACA
  TTTGAAATATATAGGGTGCCCTTTGGCAAAGAAAGAATAGAAGGAGTAATTAAGGACTTCGTTTTGACAT
  GGCTGGAAAATAGACCATCTCCTTCAACCATTTGGAGATTCTATCAGGAGATGGCCAAAGTAATCAAGGA
  CTTCCACATGGTGTCTCAGGAGATCTGTGATGGCGTTCTTAAAAACCAACAGCTGATGGCAAAGCTAAAG
  AAAAGCAAGTTTGAAGTCCTGGTGTCTGATCCAGTATTTCCTTGTGGCGATATAGTAGCTTTAAAACTTG
  GAATTCCATTTATGTACTCCTTGAGGTTTTCTCCAGCCTCAACAGTGGAAAAGCACTGTGGGAAGGTACC
  ATACCCTCCTTCCTATGTTCCTGCTGTTTTATCAGAACTCACCGACCAAATGTCTTTCACTGACAGAATA
  AGAAATTTCATCTCCTACCACCTACAGGACTACATGTTTGAAACTCTTTGGAAATCATGGGATTCATACT
  ATAGTAAAGCTTTAGATGGTAGCCATTGGTTAAATATTAAGATTATTCTAGAAGAGTTGATTCAAAGAAA
  TCACAATGTGACTGTACTGGCTTCATCAGCAACTCTATTCATCAACTCCAATCCCGATTCTCCTGTGAAT
  TTTGAAGTGATACCTGTTTCCTACAAGAAGAGCAATATAGATTCCTTAATTGAGCATATGATAATGCTGT
  GGATTGACCATAGACCAACTCCTCTCACAATATGGGCTTTCTACAAAGAACTAGGAAAACTTCTAGACAC
  TTTCTTTCAAATTAACATACAACTCTGTGATGGTGTACTAAAGAACCCAAAGTTGATGGCAAGACTTCAG
  AAAGGTGGTTTTGATGTGTTGGTAGCAGACCCAGTAACAATCTGTGGTGATCTTGTTGCTCTGAAATTAG
  GAATTCCATTTATGTACACATTGAGGTTCTCTCCAGCATCAACAGTGGAGAGACACTGTGGGAAAATCCC
  AGCACCAGTCTCCTATGTACCGGCAGCCTTATCAGAGCTCACTGACCAGATGACCTTTGGTGAAAGGATT
  AAAAATACCATATCTTATTCTCTGCAAGACTATATATTTCAGTCCTACTGGGGAGAATGGAATTCATACT
  ATAGCAAAATTTTAGGAAGACCCACTACGTTATGTGAGACTATGGGGAAAGCTGAAATTTGGTTAATCCG
  AACATATTGGGATTTTGAATTTCCTCGTCCATACTTACCTAATTTTGAGTTTGTTGGAGGATTGCACTGC
  AAACCTGCCAAACCTTTACCTAAGGTTTTATGGAGATACAAAGGAAAGAAACCAGCCACATTAGGAAACA
  ATACTCAGCTCTTTGATTGGATACCCCAGAATGATCTTCTTGGACATCCCAAAACCAAAGCTTTTATCAC
  TCATGGTGGAACTAATGGGATCTACGAAGCTATTTACCACGGAGTCCCTATGGTGGGAGTTCCCATGTTT
  GCTGATCAGCCTGATAACATTGCTCACATGAAGGCCAAAGGAGCAGCTGTGGAAGTGAACCTAAACACAA
  TGACAAGTGTGGATTTGCTTAGCGCTTTGAGAACAGTCATTAATGAACCTTCTTATAAAGAGAATGCTAT
  GAGGTTATCAAGAATTCACCATGATCAACCTGTAAAGCCCCTGGATCGAGCAGTCTTCTGGATCGAGTTT
  GTCATGCGCCACAAAGGAGCCAAGCACCTTCGGGTTGCAGCCCATGACCTCACCTGGTTCCAGTACCACT
  CTTTGGATGTAATTGGGTTCTTGCTGGTCTGTGTGACAACGGCTATATTTTTGGTCATACAATGTTGTTT
  GTTTTCCTGTCAAAAATTTGGTAAGATAGGAAAGAAGAAAAAAAGAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234730 representing NM_001252274
  Red=Cloning site Green=Tags(s)
  
  MLNNLLLFSLQISLIGTTLGGNVLIWPMEGSHWLNVKIIIDELIKKEHNVTVLVASGALFITPTSNPSLT
  FEIYRVPFGKERIEGVIKDFVLTWLENRPSPSTIWRFYQEMAKVIKDFHMVSQEICDGVLKNQQLMAKLK
  KSKFEVLVSDPVFPCGDIVALKLGIPFMYSLRFSPASTVEKHCGKVPYPPSYVPAVLSELTDQMSFTDRI
  RNFISYHLQDYMFETLWKSWDSYYSKALDGSHWLNIKIILEELIQRNHNVTVLASSATLFINSNPDSPVN
  FEVIPVSYKKSNIDSLIEHMIMLWIDHRPTPLTIWAFYKELGKLLDTFFQINIQLCDGVLKNPKLMARLQ
  KGGFDVLVADPVTICGDLVALKLGIPFMYTLRFSPASTVERHCGKIPAPVSYVPAALSELTDQMTFGERI
  KNTISYSLQDYIFQSYWGEWNSYYSKILGRPTTLCETMGKAEIWLIRTYWDFEFPRPYLPNFEFVGGLHC
  KPAKPLPKVLWRYKGKKPATLGNNTQLFDWIPQNDLLGHPKTKAFITHGGTNGIYEAIYHGVPMVGVPMF
  ADQPDNIAHMKAKGAAVEVNLNTMTSVDLLSALRTVINEPSYKENAMRLSRIHHDQPVKPLDRAVFWIEF
  VMRHKGAKHLRVAAHDLTWFQYHSLDVIGFLLVCVTTAIFLVIQCCLFSCQKFGKIGKKKKRE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252274
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252274.1, NP_001239203.1
• RefSeq Size: 3154 bp
• RefSeq ORF: 2082 bp
• Locus ID: 10941
• UniProt ID: Q9Y4X1
• Cytogenetics: 4q13.3
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• Gene Summary: The protein encoded by this gene belongs to the UDP-glycosyltransferase family, members of which catalyze biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase water solubility and enhance excretion. They are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This enzyme is expressed in the olfactory neuroepithelium, which lines the posterior nasal cavity and is exposed to a wide range of odorants and airborne toxic compounds. Hence, this protein has been suggested to be involved in clearing lipophilic odorant molecules from the sensory epithelium. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. This gene shares exon structure with the UDP glucuronosyltransferase 2A2 family member, which encodes N-terminally distinct isoforms. [provided by RefSeq, Jul 2014]