TRAK1 (NM_001265609) Human Tagged ORF Clone

CAT#: RG234686

TRAK1 (tGFP-tagged) - Homo sapiens trafficking protein, kinesin binding 1 (TRAK1), transcript variant 4

Product Images

Circular map for RG234686

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRAK1
• Synonyms: EIEE68; MILT1; OIP106
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234686 representing NM_001265609
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAATTTATCGAAGCGGATTATTATGAACTAGACTGGTATTATGAAGAATGCTCGGATGTTTTAT
  GTGCTGAAAGAGTTGGCCAGATGACTAAGACATATAATGACATAGATGCTGTCACTCGGCTTCTTGAGGA
  GAAAGAGCGGGATTTAGAATTGGCCGCTCGCATCGGCCAGTCGTTGTTGAAGAAGAACAAGACCCTAACC
  GAGAGGAACGAGCTGCTGGAGGAGCAGGTGGAACACATCAGGGAGGAGGTGTCTCAGCTCCGGCATGAGC
  TGTCCATGAAGGATGAGCTGCTTCAGTTCTACACCAGCGCTGCGGAGGAGAGTGAGCCCGAGTCCGTTTG
  CTCAACCCCGTTGAAGAGGAATGAGTCGTCCTCCTCAGTCCAGAATTACTTTCATTTGGATTCTCTTCAA
  AAGAAGCTGAAAGACCTTGAAGAGGAGAATGTTGTACTTCGATCCGAGGCCAGCCAGCTGAAGACAGAGA
  CCATCACCTATGAGGAGAAGGAGCAGCAGCTGGTCAATGACTGCGTGAAGGAGCTGAGGGATGCCAATGT
  CCAGATTGCTAGTATCTCAGAGGAACTGGCCAAGAAGACGGAAGATGCTGCCCGCCAGCAAGAGGAGATC
  ACACACCTGCTATCGCAAATAGTTGATTTGCAGAAAAAGGCAAAAGCTTGCGCAGTGGAAAATGAAGAAC
  TTGTCCAGCATCTGGGGGCTGCTAAGGATGCCCAGCGGCAGCTCACAGCCGAGCTGCGTGAGCTGGAGGA
  CAAGTACGCAGAGTGCATGGAGATGCTGCATGAGGCGCAGGAGGAGCTGAAGAACCTCCGGAACAAAACC
  ATGCCCAATACCACGTCTCGGCGCTACCACTCACTGGGCCTGTTTCCCATGGATTCCTTGGCAGCAGAGA
  TTGAGGGAACGATGCGCAAGGAGCTGCAGTTGGAAGAGGCCGAGTCTCCAGACATCACTCACCAGAAGCG
  TGTCTTTGAGACAGTAAGAAACATCAACCAGGTTGTCAAGCAGAGATCTCTGACCCCTTCTCCCATGAAC
  ATCCCCGGCTCCAACCAGTCCTCGGCCATGAACTCCCTCCTGTCCAGCTGCGTCAGCACCCCCCGGTCCA
  GCTTCTACGGCAGCGACATAGGCAACGTCGTCCTCGACAACAAGACCAACAGCATCATTCTGGAAACAGA
  GGCAGCCGACCTGGGAAACGATGAGCGGAGTAAGAAGCCGGGGACGCCGGGCACCCCAGGCTCCCACGAC
  CTGGAGACGGCGCTGAGGCGGCTGTCCCTGCGCCGGGAGAACTACCTCTCGGAGAGGAGGTTCTTTGAGG
  AGGAGCAAGAGAGGAAGCTCCAGGAGCTGGCGGAGAAGGGCGAGCTGCGCAGCGGCTCCCTCACACCCAC
  TGAGAGCATCATGTCCCTGGGCACGCACTCCCGCTTCTCCGAGTTCACCGGCTTCTCTGGCATGTCCTTC
  AGCAGCCGCTCCTACCTGCCTGAGAAGCTCCAGATCGTGAAGCCGCTGGAAGGTTCCGCCACACTTCACC
  ACTGGCAGCAGTTGGCCCAACCTCACCTTGGGGGCATCCTGGACCCCCGGCCCGGTGTGGTCACCAAGGG
  CTTCCGGACGCTGGATGTTGACCTGGACGAAGTGTACTGCCTTAACGACTTTGAAGAAGATGACACAGGT
  GACCACATTTCTCTCCCACGCCTAGCTACCTCCACTCCAGTTCAGCACCCAGAGACCTCAGGTGAGAGGT
  CCCAAGCACGTGTGACTGTCTCAGGCAGCAGAAGTTACCCGAGCCGGCCTCAGGCTTCCCCAGAGGAGAT
  GCAGGAGCCGCCAGCGGCCACGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGGGTCTGGTGAGGGCACC
  ACGATAAGTCCTGTAAACTTGGCACCTTTCCCGGAGGCAGAGTTTTGGGCCATTCTCACCTCTGTTCCAG
  GCACCATCCGTAGTGGTTCTCTGTCTGTAGCTTCCGCTCGTCTGTGTGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234686 representing NM_001265609
  Red=Cloning site Green=Tags(s)
  
  MQKFIEADYYELDWYYEECSDVLCAERVGQMTKTYNDIDAVTRLLEEKERDLELAARIGQSLLKKNKTLT
  ERNELLEEQVEHIREEVSQLRHELSMKDELLQFYTSAAEESEPESVCSTPLKRNESSSSVQNYFHLDSLQ
  KKLKDLEEENVVLRSEASQLKTETITYEEKEQQLVNDCVKELRDANVQIASISEELAKKTEDAARQQEEI
  THLLSQIVDLQKKAKACAVENEELVQHLGAAKDAQRQLTAELRELEDKYAECMEMLHEAQEELKNLRNKT
  MPNTTSRRYHSLGLFPMDSLAAEIEGTMRKELQLEEAESPDITHQKRVFETVRNINQVVKQRSLTPSPMN
  IPGSNQSSAMNSLLSSCVSTPRSSFYGSDIGNVVLDNKTNSIILETEAADLGNDERSKKPGTPGTPGSHD
  LETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGSLTPTESIMSLGTHSRFSEFTGFSGMSF
  SSRSYLPEKLQIVKPLEGSATLHHWQQLAQPHLGGILDPRPGVVTKGFRTLDVDLDEVYCLNDFEEDDTG
  DHISLPRLATSTPVQHPETSGERSQARVTVSGSRSYPSRPQASPEEMQEPPAATEEEEEEEEEEGSGEGT
  TISPVNLAPFPEAEFWAILTSVPGTIRSGSLSVASARLCG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001265609
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001265609.1, NP_001252538.1
• RefSeq Size: 4683 bp
• RefSeq ORF: 2013 bp
• Locus ID: 22906
• Cytogenetics: 3p22.1
• Protein Families: Transcription Factors
• Gene Summary: Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (PubMed:18675823). Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes (PubMed:24995978). TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).[UniProtKB/Swiss-Prot Function]