DLGAP1 (NM_001242765) Human Tagged ORF Clone

CAT#: RG234599

DLGAP1 (tGFP-tagged) - Homo sapiens discs, large (Drosophila) homolog-associated protein 1 (DLGAP1), transcript variant 7

Product Images

Circular map for RG234599

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DLGAP1
• Synonyms: DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; DLGAP1A; DLGAP1B; GKAP; SAPAP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234599 representing NM_001242765
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTAATTTTCCATAAAGACATTCTGTTTGGCATTCCAGCTAATAAGGTTCCACAAGATGAATGGA
  CAGGGTACACCCCACGAGGTAAAGATGATGAAATTCCATGCCGAAGAATGCGGAGTGGCAGTTATATCAA
  GGCCATGGGGGATGAAGACAGTGGAGACTCAGACACGAGTCCTAAGCCTTCTCCAAAAGTTGCTGCGCGG
  AGAGAAAGCTATCTCAAGGCTACTCAGCCATCCCTTACAGAACTCACCACACTCAAAATCTCCAATGAAC
  ACTCACCCAAACTCCAGATCCGGAGTCATAGTTACCTGAGGGCAGTGAGTGAAGTCTCCATCAACCGGAG
  CCTGGACAGCCTGGACCCTGCAGGCTTGCTCACATCACCAAAGTTCCGCTCCAGGAATGAGAGCTACATG
  CGAGCCATGAGCACCATCAGCCAGGTGAGCGAGATGGAAGTGAACGGGCAGTTCGAGTCCGTGTGCGAGT
  CCGTGTTCAGCGAGCTGGAGTCGCAGGCCGTGGAAGCGCTGGACCTGCCCATGCCCGGCTGCTTCCGCAT
  GCGGAGCCACAGCTATGTGCGGGCCATTGAGAAAGGCTGCTCCCAGGACGACGAGTGCGTGTCCCTGAGG
  TCGTCCTCGCCGCCGCGCACCACCACCACCGTTAGGACCATCCAGAGCAGCACGGTGTCATCTTGCATTA
  CAACATATAAGAAGACACCACCTCCAGTCCCACCCAGAACTACCACGAAACCTTTCATTTCTATCACAGC
  CCAGAGTAGCACAGAGTCAGCCCAGGATGCCTACATGGACGGACAGGGCCAGCGAGGAGATATTATCAGC
  CAGTCTGGACTCAGCAACTCCACCGAGAGCCTGGACAGTATGAAGGCTCTGACAGCCGCCATCGAAGCTG
  CAAACGCCCAGATCCATGGCCCTGCCAGTCAACACATGGGCAATAACACTGCCACCGTCACCACCACGAC
  TACCATAGCCACCGTCACCACGGAGGACAGGAAGAAGGACCACTTTAAGAAAAATCGATGCCTGTCTATC
  GGGATACAGGTGGATGATGCTGAAGAACCTGACAAAACAGGGGAGAATAAAGCACCCAGTAAGTTCCAGT
  CCGTGGGAGTGCAAGTAGAAGAAGAGAAGTGCTTCCGCAGGTTCACTCGATCCAACAGTGTGACGACAGC
  AGTACAGGCCGACCTGGACTTCCATGATAATCTGGAAAATTCTCTGGAATCTATAGAGGACAATTCGTGT
  CCTGGCCCCATGGCCAGACAGTTCTCCCGCGATGCCAGCACCTCCACAGTCAGCATTCAGGGCTCAGGAA
  ACCATTACCATGCCTGTGCCGCCGATGATGACTTTGACACGGATTTTGACCCCTCTATTCTGCCTCCTCC
  GGACCCCTGGATTGACTCTATCACTGAAGACCCTCTGGAGGCCGTGCAAAGGTCAGTGTGCCACCGGGAT
  GGCCACTGGTTCCTGAAGCTTCTCCAGGCAGAGCGAGACCGCATGGAGGGGTGGTGTCAACAGATGGAGC
  GGGAAGAACGGGAAAACAACCTGCCCGAAGACATTCTAGGAAAAATCCGAACCGCAGTGGGCAGTGCCCA
  ACTTCTCATGGCCCAGAAATTCTACCAGTTCAGAGAACTGTGTGAAGAAAACCTGAATCCTAATGCTCAT
  CCAAGACCCACCTCCCAGGATTTGGCGGGGTTTTGGGACATGCTGCAGTTGTCCATAGAAAATATTAGTA
  TGAAATTTGATGAACTTCATCAGTTAAAGGCCAATAATTGGAAACAGATGGATCCTCTTGACAAGAAGGT
  AGAACAATGTAGATTTTGTATGGTTCATTTAAAAACCTGCACAAACACTGGACAGTCTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234599 representing NM_001242765
  Red=Cloning site Green=Tags(s)
  
  MNLIFHKDILFGIPANKVPQDEWTGYTPRGKDDEIPCRRMRSGSYIKAMGDEDSGDSDTSPKPSPKVAAR
  RESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEVSINRSLDSLDPAGLLTSPKFRSRNESYM
  RAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPMPGCFRMRSHSYVRAIEKGCSQDDECVSLR
  SSSPPRTTTTVRTIQSSTVSSCITTYKKTPPPVPPRTTTKPFISITAQSSTESAQDAYMDGQGQRGDIIS
  QSGLSNSTESLDSMKALTAAIEAANAQIHGPASQHMGNNTATVTTTTTIATVTTEDRKKDHFKKNRCLSI
  GIQVDDAEEPDKTGENKAPSKFQSVGVQVEEEKCFRRFTRSNSVTTAVQADLDFHDNLENSLESIEDNSC
  PGPMARQFSRDASTSTVSIQGSGNHYHACAADDDFDTDFDPSILPPPDPWIDSITEDPLEAVQRSVCHRD
  GHWFLKLLQAERDRMEGWCQQMEREERENNLPEDILGKIRTAVGSAQLLMAQKFYQFRELCEENLNPNAH
  PRPTSQDLAGFWDMLQLSIENISMKFDELHQLKANNWKQMDPLDKKVEQCRFCMVHLKTCTNTGQSK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242765
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242765.1, NP_001229694.1
• RefSeq Size: 2253 bp
• RefSeq ORF: 1884 bp
• Locus ID: 9229
• UniProt ID: O14490
• Cytogenetics: 18p11.31
• Gene Summary: Part of the postsynaptic scaffold in neuronal cells.[UniProtKB/Swiss-Prot Function]