ZNF382 (NM_001256838) Human Tagged ORF Clone

CAT#: RG234413

ZNF382 (tGFP-tagged) - Homo sapiens zinc finger protein 382 (ZNF382), transcript variant 2

Product Images

Circular map for RG234413

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF382
• Synonyms: KS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234413 representing NM_001256838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAGGGATCAGTGTCATTCAAGGATGTGACTGTGGACTTCACCCAGGAGGAGTGGCAGCAACTAG
  ACCCTGCTCAGAAGGCGCTTTACAGGGATGTGATGTTGGAAAACTATTGCCACTTCGTATCTGTGGGGTT
  TCACATGGCTAAGCCTGATATGATCCGCAAGTTGGAACAAGGAGAAGAGCTATGGACACAGAGAATTTTT
  CCAAGTTACAGCTACCTAGAAGAAGATGGGAAAACTGAAGATGTCTTAGTGAAGTTCAAAGAATACCAAG
  ACAGGCATTCTAGACCCCTCATATTCATCAACCACAAAAAACTAATTAAGGAGAGAAGTAATATTTATGG
  TAAAACATTTACTCTAGGCAAGAACCGTATTTCAAAAACAATACTATGTGAATATAAACCTGATGGAAAA
  GTTTTGAAAAATATTTCAGAACTAGTCATTAGAAATATAAGCCCCATAAAAGAGAAGTTTGGTGACAGTA
  CTGGATGGGAGAAATCACTCCTCAATACCAAGCATGAGAAAATTCATCCTGCAGTGAATCTCCATAAACA
  AACAGAAAGAGTTCTCAGTGGTAAACAGGAGCTTATTCAGCATCAGAAGGTTCAAGCTCCAGAGCAACCA
  TTTGACCATAATGAATGTGAAAAATCCTTCCTGATGAAAGGAATGCTATTTACACATACTAGAGCTCACA
  GAGGAGAAAGAACCTTTGAATACAATAAAGATGGAATTGCCTTCATAGAAAAGTCAAGCCTCAGTGTCCA
  TCCAAGTAATCTTATGGAAAAGAAGCCCTCTGCCTACAACAAATATGGGAAATTCCTCTGCAGAAAGCCT
  GTTTTTATTATGCCTCAGAGACCTCAAACAGAAGAGAAACCCTTTCACTGTCCTTACTGTGGGAATAACT
  TTAGAAGGAAGTCATACCTCATTGAACATCAGCGAATTCACACAGGTGAAAAACCTTATGTTTGCAATCA
  ATGTGGAAAGGCCTTCCGTCAGAAGACAGCCCTCACCCTTCATGAGAAAACACATATAGAGGGGAAACCC
  TTTATTTGTATCGATTGTGGGAAGTCCTTCCGCCAGAAGGCCACCCTCACTAGACATCACAAAACACATA
  CGGGGGAGAAAGCCTATGAATGTCCTCAGTGTGGAAGTGCCTTTAGGAAGAAGTCATACCTCATTGATCA
  CCAGAGAACTCACACAGGAGAGAAACCGTATCAGTGTAATGAGTGTGGGAAGGCATTTATCCAGAAGACA
  ACCCTCACTGTTCATCAGAGAACTCACACAGGAGAGAAACCCTATATTTGCAATGAATGTGGGAAGTCCT
  TCTGCCAAAAGACAACCCTCACTCTCCACCAGAGAATTCACACGGGGGAAAAACCCTATATTTGTAATGA
  ATGTGGGAAGTCCTTCCGCCAGAAGGCAATCCTCACTGTTCATCACAGAATACATACAGGAGAAAAATCC
  AATGGGTGTCCTCAGTGTGGGAAAGCCTTCAGTAGGAAATCAAACCTCATTCGCCATCAGAAAACTCACA
  CAGGCGAGAAACCATATGAATGTAAACAGTGTGGGAAGTTCTTCAGTTGTAAGTCAAACCTCATTGTCCA
  TCAGAAAACTCACAAGGTAGAAACCACGGGAATTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234413 representing NM_001256838
  Red=Cloning site Green=Tags(s)
  
  MSQGSVSFKDVTVDFTQEEWQQLDPAQKALYRDVMLENYCHFVSVGFHMAKPDMIRKLEQGEELWTQRIF
  PSYSYLEEDGKTEDVLVKFKEYQDRHSRPLIFINHKKLIKERSNIYGKTFTLGKNRISKTILCEYKPDGK
  VLKNISELVIRNISPIKEKFGDSTGWEKSLLNTKHEKIHPAVNLHKQTERVLSGKQELIQHQKVQAPEQP
  FDHNECEKSFLMKGMLFTHTRAHRGERTFEYNKDGIAFIEKSSLSVHPSNLMEKKPSAYNKYGKFLCRKP
  VFIMPQRPQTEEKPFHCPYCGNNFRRKSYLIEHQRIHTGEKPYVCNQCGKAFRQKTALTLHEKTHIEGKP
  FICIDCGKSFRQKATLTRHHKTHTGEKAYECPQCGSAFRKKSYLIDHQRTHTGEKPYQCNECGKAFIQKT
  TLTVHQRTHTGEKPYICNECGKSFCQKTTLTLHQRIHTGEKPYICNECGKSFRQKAILTVHHRIHTGEKS
  NGCPQCGKAFSRKSNLIRHQKTHTGEKPYECKQCGKFFSCKSNLIVHQKTHKVETTGIQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256838
• ORF Size: 1647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256838.2
• RefSeq Size: 2819 bp
• RefSeq ORF: 1650 bp
• Locus ID: 84911
• UniProt ID: Q96SR6
• Cytogenetics: 19q13.12
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a KRAB domain zinc finger transcription factor (KZNF). KZNFs play critical roles in the regulation of many cellular processes including differentiation, proliferation and apoptosis. The encoded protein inhibits activating protein 1 (AP-1) and nuclear factor kappa-B (NF-kB) signaling and may function as a tumor suppressor in multiple carcinomas. This gene is found in a cluster with other zinc finger protein genes on the long arm of chromosome 19, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]