PDE1A (NM_001258314) Human Tagged ORF Clone

CAT#: RG234298

PDE1A (tGFP-tagged) - Homo sapiens phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 5

Product Images

Circular map for RG234298

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE1A
• Synonyms: CAM-PDE-1A; CAM-PDE 1A; HCAM-1; HCAM1; HSPDE1A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234298 representing NM_001258314
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAAAAGATAAACAAACTATTTTGCTTTAATTTTCTAGTTCAGTGTTTTAGGGGTAAATCAAAAC
  CATCCAAATGTCAGATCAGAAAGAAAGTTAAAAATCATATAGAAAGACTTCTGGATACTGAAGATGAGCT
  CAGTGACATTCAGACTGACTCAGTCCCATCTGAAGTCCGGGACTGGTTGGCTTCTACCTTTACACGGAAA
  ATGGGGATGACAAAAAAGAAACCTGAGGAAAAACCAAAATTTCGGAGCATTGTGCATGCTGTTCAAGCTG
  GAATTTTTGTGGAAAGAATGTACCGAAAAACATATCATATGGTTGGTTTGGCATATCCAGCAGCTGTCAT
  CGTAACATTAAAGGATGTTGATAAATGGTCTTTCGATGTATTTGCCCTAAATGAAGCAAGTGGAGAGCAT
  AGTCTGAAGTTTATGATTTATGAACTGTTTACCAGATATGATCTTATCAACCGTTTCAAGATTCCTGTTT
  CTTGCCTAATCACCTTTGCAGAAGCTTTAGAAGTTGGTTACAGCAAGTACAAAAATCCATATCACAATTT
  GATTCATGCAGCTGATGTCACTCAAACTGTGCATTACATAATGCTTCATACAGGTATCATGCACTGGCTC
  ACTGAACTGGAAATTTTAGCAATGGTCTTTGCTGCTGCCATTCATGATTATGAGCATACAGGGACAACAA
  ACAACTTTCACATTCAGACAAGGTCAGATGTTGCCATTTTGTATAATGATCGCTCTGTCCTTGAGAATCA
  CCACGTGAGTGCAGCTTATCGACTTATGCAAGAAGAAGAAATGAATATCTTGATAAATTTATCCAAAGAT
  GACTGGAGGGATCTTCGGAACCTAGTGATTGAAATGGTTTTATCTACAGACATGTCAGGTCACTTCCAGC
  AAATTAAAAATATAAGAAACAGTTTGCAGCAGCCTGAAGGGATTGACAGAGCCAAAACCATGTCCCTGAT
  TCTCCACGCAGCAGACATCAGCCACCCAGCCAAATCCTGGAAGCTGCATTATCGGTGGACCATGGCCCTA
  ATGGAGGAGTTTTTCCTGCAGGGAGATAAAGAAGCTGAATTAGGGCTTCCATTTTCCCCACTTTGTGATC
  GGAAGTCAACCATGGTGGCCCAGTCACAAATAGGTTTCATCGATTTCATAGTAGAGCCAACATTTTCTCT
  TCTGACAGACTCAACAGAGAAAATTGTTATTCCTCTTATAGAGGAAGCCTCAAAAGCCGAAACTTCTTCC
  TATGTGGCAAGCAGCTCAACCACCATTGTGGGGTTACACATTGCTGATGCACTAAGACGATCAAATACAA
  AAGGCTCCATGAGTGATGGGTCCTATTCCCCAGACTACTCCCTTGCAGCAGTGGACCTGAAGAGTTTCAA
  GAACAACCTGGTGGACATCATTCAGCAGAACAAAGAGAGGTGGAAAGAGTTAGCTGCACAAGAAGCAAGA
  ACCAGTTCACAGAAGTGTGAGTTTATTCATCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234298 representing NM_001258314
  Red=Cloning site Green=Tags(s)
  
  MGKKINKLFCFNFLVQCFRGKSKPSKCQIRKKVKNHIERLLDTEDELSDIQTDSVPSEVRDWLASTFTRK
  MGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKTYHMVGLAYPAAVIVTLKDVDKWSFDVFALNEASGEH
  SLKFMIYELFTRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWL
  TELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILINLSKD
  DWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWKLHYRWTMAL
  MEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSS
  YVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQEAR
  TSSQKCEFIHQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258314
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258314.1, NP_001245243.1
• RefSeq Size: 2015 bp
• RefSeq ORF: 1506 bp
• Locus ID: 5136
• UniProt ID: P54750
• Cytogenetics: 2q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction
• Gene Summary: Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]