Bcl rambo (BCL2L13) (NM_001270726) Human Tagged ORF Clone

CAT#: RG233224

BCL2L13 (tGFP-tagged) - Homo sapiens BCL2-like 13 (apoptosis facilitator) (BCL2L13), transcript variant 2

Product Images

Circular map for RG233224

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bcl rambo
• Synonyms: BCL-RAMBO; Bcl2-L-13; MIL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233224 representing NM_001270726
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGTAGTTCAGGTCCCGCCCCGACGCCGGCCGTGACGAAGGCACGCCGGGGTGACCTCACCCTCC
  AACATGGCGGCGGCGGTAGATTAGGGCCGCGGGTCGGAGCACTCACCGCCGCTGGGGGACCCTGTCGGAA
  GCAACTGCCGCCGCCGCCTCTTTCATCTCTTCTGGGGCAGGGGCCAGGGCCAGGGGTTCAACTAGATATA
  GCTTCACAATCTCTGGATCAAGAAATTTTATTAAAAGTTAAAACTGAAATTGAAGAAGAGCTAAAATCTC
  TGGACAAAGAAATTTCTGAAGCCTTCACCAGCACAGGCTTTGACCGTCACACTTCTCCAGTGTTCAGCCC
  TGCCAATCCAGAAAGCTCAATGGAAGACTGCTTGGCCCATCTTGGAGAAAAAGTGTCCCAGGAACTGAAA
  GAGCCTCTCCATAAAGCATTGCAAATGCTCCTGAGCCAGCCAGTGACATATCAGGCATTTCGGGAATGTA
  CACTGGAGACCACAGTTCATGCCAGCGGCTGGAATAAGATTTTGGTGCCTCTGGTTTTGCTACGACAAAT
  GCTTTTGGAATTGACAAGACGTGGTCAAGAACCTTTGAGCGCACTGCTGCAGTTTGGCGTGACATACCTG
  GAGGACTATTCGGCAGAGTACATCATTCAGCAAGGTGGCTGGGGCACTGTGTTTAGTCTTGAGTCAGAGG
  AGGAGGAATACCCTGGAATCACTGCAGAAGATAGCAATGACATTTACATCCTGCCCAGCGACAACTCTGG
  ACAAGTCAGTCCCCCAGAGTCTCCAACTGTGACCACTTCCTGGCAGTCTGAGAGCTTACCTGTGTCACTG
  TCAGCTAGCCAGAGTTGGCACACAGAAAGCCTGCCAGTGTCACTAGGCCCTGAGTCCTGGCAGCAGATTG
  CAATGGATCCTGAAGAAGTGAAAAGCTTAGACAGCAACGGAGCTGGAGAGAAGAGTGAGAACAACTCCTC
  TAATTCTGACATTGTGCACGTGGAGAAAGAAGAGGTGCCCGAGGGCATGGAAGAGGCTGCTGTGGCTTCT
  GTGGTCTTGCCAGCGCGGGAGCTGCAAGAGGCACTTCCTGAAGCCCCAGCTCCCTTGCTTCCACATATCA
  CTGCCACCTCCCTGCTGGGGACAAGGGAACCTGACACAGAAGTGATCACAGTTGAGAAATCCAGCCCTGC
  TACATCTCTGTTTGTAGAACTTGATGAAGAAGAGGTGAAAGCAGCAACAACTGAACCTACTGAAGTGGAG
  GAGGTGGTCCCCGCACTGGAACCCACAGAAACGCTGCTGAGTGAGAAGGAGATAAACGCAAGGGAAGAGA
  GCCTTGTGGAAGAGCTGTCCCCTGCCAGCGAGAAGAAGCCCGTGCCGCCGTCTGAGGGCAAGTCTAGACT
  GTCCCCCGCCGGTGAGATGAAGCCCATGCCGCTGTCTGAGGGCAAGTCTATACTGCTGTTTGGAGGGGCT
  GCTGCTGTTGCCATCCTGGCAGTGGCCATCGGGGTAGCCCTGGCTCTGAGAAAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233224 representing NM_001270726
  Red=Cloning site Green=Tags(s)
  
  MSGSSGPAPTPAVTKARRGDLTLQHGGGGRLGPRVGALTAAGGPCRKQLPPPPLSSLLGQGPGPGVQLDI
  ASQSLDQEILLKVKTEIEEELKSLDKEISEAFTSTGFDRHTSPVFSPANPESSMEDCLAHLGEKVSQELK
  EPLHKALQMLLSQPVTYQAFRECTLETTVHASGWNKILVPLVLLRQMLLELTRRGQEPLSALLQFGVTYL
  EDYSAEYIIQQGGWGTVFSLESEEEEYPGITAEDSNDIYILPSDNSGQVSPPESPTVTTSWQSESLPVSL
  SASQSWHTESLPVSLGPESWQQIAMDPEEVKSLDSNGAGEKSENNSSNSDIVHVEKEEVPEGMEEAAVAS
  VVLPARELQEALPEAPAPLLPHITATSLLGTREPDTEVITVEKSSPATSLFVELDEEEVKAATTEPTEVE
  EVVPALEPTETLLSEKEINAREESLVEELSPASEKKPVPPSEGKSRLSPAGEMKPMPLSEGKSILLFGGA
  AAVAILAVAIGVALALRKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270726
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270726.1, NP_001257655.1
• RefSeq Size: 4961 bp
• RefSeq ORF: 1530 bp
• Locus ID: 23786
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. Overexpression of the encoded protein results in apoptosis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]