Bcl rambo (BCL2L13) Rabbit Polyclonal Antibody

CAT#: TA306100

Rabbit Polyclonal Bcl-rambo Antibody

Product Images

Western blot analysis of Bcl-rambo in K562 cell lysate with Bcl-rambo antibody at (A) 2 and (B) 4 ug/ml.

• 

  Immunohistochemistry of Bcl-rambo in human heart tissue with Bcl-rambo antibody at 10 ug/ml.

• 

  Immunofluorescence of Bcl-rambo in Human Heart cells with Bcl-rambo antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 2 - 4 ug/mL, ICC: 10 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Bcl-rambo antibody was raised against a 15 amino acid peptide from near the center of human Bcl-rambo.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BCL2 like 13
• Database Link:
  AAH07658
  Entrez Gene 23786 Human
  UniProt ID Q9BXK5
• Background: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains (reviewed in 2 and 3). A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2 (4). Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways (4).
• Synonyms: BCL-RAMBO; Bcl2-L-13; MIL1