ATP2C1 (NM_001199182) Human Tagged ORF Clone

CAT#: RG233184

  • TrueORF®

ATP2C1 (tGFP-tagged) - Homo sapiens ATPase, Ca++ transporting, type 2C, member 1 (ATP2C1), transcript variant 8

ORF Plasmid: DDK tGFP


  "NM_001199182" in other vectors (2)

Reconstitution Protocol

USD 1,151.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "ATP2C1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol ATP2C1
Synonyms ATP2C1A; BCPM; HHD; hSPCA1; PMR1; SPCA1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG233184 representing NM_001199182
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATAGTCTGTTGCCTCCATCTAGATTCTCTTATTTCAAAAAATATCCTCTCCATGCAATTAGGAGAT
ATTTATCGACGCTGAGAAACCAAAGAGCCGAAGAACAGGTTGCACGTTTTCAAAAAATACCTAATGGTGA
AAATGAGACAATGATTCCTGTATTGACATCAAAAAAAGCAAGTGAATTACCAGTCAGTGAAGTTGCAAGC
ATTCTCCAATTTAAAAATCCCCTTATTATGCTGCTTCTGGCTTCTGCAGTCATCAGTGTTTTAATGCATC
AGTTTGATGATGCCGTCAGTATCACTGTGGCAATACTTATCGTTGTTACAGTTGCCTTTGTTCAGGAATA
TCGTTCAGAAAAATCTCTTGAAGAATTGAGTAAACTTGTGCCACCAGAATGCCATTGTGTGCGTGAAGGA
AAATTGGAGCATACACTTGCCCGAGACTTGGTTCCAGGTGATACAGTTTGCCTTTCTGTTGGGGATAGAG
TTCCTGCTGACTTACGCTTGTTTGAGGCTGTGGATCTTTCCATTGATGAGTCCAGCTTGACAGGTGAGAC
AACGCCTTGTTCTAAGGTGACAGCTCCTCAGCCAGCTGCAACTAATGGAGATCTTGCATCGAGAAGTAAC
ATTGCCTTTATGGGAACACTGGTCAGATGTGGCAAAGCAAAGGGTGTTGTCATTGGAACAGGAGAAAATT
CTGAATTTGGGGAGGTTTTTAAAATGATGCAAGCAGAAGAGGCACCAAAAACCCCTCTGCAGAAGAGCAT
GGACCTCTTAGGAAAACAACTTTCCTTTTACTCCTTTGGTATAATAGGAATCATCATGTTGGTTGGCTGG
TTACTGGGAAAAGATATCCTGGAAATGTTTACTATTAGTGTAAGTTTGGCTGTAGCAGCAATTCCTGAAG
GTCTCCCCATTGTGGTCACAGTGACGCTAGCTCTTGGTGTTATGAGAATGGTGAAGAAAAGGGCCATTGT
GAAAAAGCTGCCTATTGTTGAAACTCTGGGCTGCTGTAATGTGATTTGTTCAGATAAAACTGGAACACTG
ACGAAGAATGAAATGACTGTTACTCACATATTTACTTCAGATGGTCTGCATGCTGAGGTTACTGGAGTTG
GCTATAATCAATTTGGGGAAGTGATTGTTGATGGTGATGTTGTTCATGGATTCTATAACCCAGCTGTTAG
CAGAATTGTTGAGGCGGGCTGTGTGTGCAATGATGCTGTAATTAGAAACAATACTCTAATGGGGAAGCCA
ACAGAAGGGGCCTTAATTGCTCTTGCAATGAAGATGGGTCTTGATGGACTTCAACAAGACTACATCAGAA
AAGCTGAATACCCTTTTAGCTCTGAGCAAAAGTGGATGGCTGTTAAGTGTGTACACCGAACACAGCAGGA
CAGACCAGAGATTTGTTTTATGAAAGGTGCTTACGAACAAGTAATTAAGTACTGTACTACATACCAGAGC
AAAGGGCAGACCTTGACACTTACTCAGCAGCAGAGAGATGTGTACCAACAAGAGAAGGCACGCATGGGCT
CAGCGGGACTCAGAGTTCTTGCTTTGGCTTCTGGTCCTGAACTGGGACAGCTGACATTTCTTGGCTTGGT
GGGAATCATTGATCCACCTAGAACTGGTGTGAAAGAAGCTGTTACAACACTCATTGCCTCAGGAGTATCA
ATAAAAATGATTACTGGAGATTCACAGGAGACTGCAGTTGCAATCGCCAGTCGTCTGGGATTGTATTCCA
AAACTTCCCAGTCAGTCTCAGGAGAAGAAATAGATGCAATGGATGTTCAGCAGCTTTCACAAATAGTACC
AAAGGTTGCAGTATTTTACAGAGCTAGCCCAAGGCACAAGATGAAAATTATTAAGTCGCTACAGAAGAAC
GGTTCAGTTGTAGCCATGACAGGAGATGGAGTAAATGATGCAGTTGCTCTGAAGGCTGCAGACATTGGAG
TTGCGATGGGCCAGACTGGTACAGATGTTTGCAAAGAGGCAGCAGACATGATCCTAGTGGATGATGATTT
TCAAACCATAATGTCTGCAATCGAAGAGGGTAAAGGGATTTATAATAACATTAAAAATTTCGTTAGATTC
CAGCTGAGCACGAGTATAGCAGCATTAACTTTAATCTCATTGGCTACATTAATGAACTTTCCTAATCCTC
TCAATGCCATGCAGATTTTGTGGATCAATATTATTATGGATGGACCCCCAGCTCAGAGCCTTGGAGTAGA
ACCAGTGGATAAAGATGTCATTCGTAAACCTCCTCGCAACTGGAAAGACAGCATTTTGACTAAAAACTTG
ATACTTAAAATACTTGTTTCATCAATAATCATTGTTTGTGGGACTTTGTTTGTCTTCTGGCGTGAGCTAC
GAGACAATGTGATTACACCTCGAGACACAACAATGACCTTCACATGCTTTGTGTTTTTTGACATGTTCAA
TGCACTAAGTTCCAGATCCCAGACCAAGTCTGTGTTTGAGATTGGACTCTGCAGTAATAGAATGTTTTGC
TATGCAGTTCTTGGATCCATCATGGGACAATTACTAGTTATTTACTTTCCTCCGCTTCAGAAGGTTTTTC
AGACTGAGAGCCTAAGCATACTGGATCTGTTGTTTCTTTTGGGTCTCACCTCATCAGTGTGCATAGTGGC
AGAAATTATAAAGAAGGTTGAAAGGAGCAGGGAAAAGATCCAGAAGCATGTTAGTTCGACATCATCATCT
TTTCTTGAAGTCTGGCTCTGGGAGAGGAGTGGACAGCAGCTGGTTGAGATACATCCCCATCTGGAGACAG
GACTGCCACTGACAGAAGATGTGAGCTGTGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG233184 representing NM_001199182
Red=Cloning site Green=Tags(s)

MDSLLPPSRFSYFKKYPLHAIRRYLSTLRNQRAEEQVARFQKIPNGENETMIPVLTSKKASELPVSEVAS
ILQFKNPLIMLLLASAVISVLMHQFDDAVSITVAILIVVTVAFVQEYRSEKSLEELSKLVPPECHCVREG
KLEHTLARDLVPGDTVCLSVGDRVPADLRLFEAVDLSIDESSLTGETTPCSKVTAPQPAATNGDLASRSN
IAFMGTLVRCGKAKGVVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGW
LLGKDILEMFTISVSLAVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTL
TKNEMTVTHIFTSDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTLMGKP
TEGALIALAMKMGLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCTTYQS
KGQTLTLTQQQRDVYQQEKARMGSAGLRVLALASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIASGVS
IKMITGDSQETAVAIASRLGLYSKTSQSVSGEEIDAMDVQQLSQIVPKVAVFYRASPRHKMKIIKSLQKN
GSVVAMTGDGVNDAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKNFVRF
QLSTSIAALTLISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSILTKNL
ILKILVSSIIIVCGTLFVFWRELRDNVITPRDTTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCSNRMFC
YAVLGSIMGQLLVIYFPPLQKVFQTESLSILDLLFLLGLTSSVCIVAEIIKKVERSREKIQKHVSSTSSS
FLEVWLWERSGQQLVEIHPHLETGLPLTEDVSCV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001199182
ORF Size 2832 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001199182.2
RefSeq Size 3615 bp
RefSeq ORF 2835 bp
Locus ID 27032
UniProt ID P98194
Cytogenetics 3q22.1
Protein Families Druggable Genome, Transmembrane
Gene Summary The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.