ODF2 (NM_153435) Human Tagged ORF Clone

CAT#: RG231435

ODF2 (tGFP-tagged) - Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 1

Product Images

Circular map for RG231435

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ODF2
• Synonyms: CT134; ODF2/1; ODF2/2; ODF84
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231435 representing NM_153435
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGCTGCCGACCGGGTGTCGGAAAAGGAGGCGGAAGCGGGGAGGAGCCGCTGCCAGAGCCAGAC
  TGCATCCGCCGCGGCGTCTCCATGGCACGACCCTGGCCTCCGACTTCAACGACTTCATAAGGCGGCGTTT
  CTGGGCGCAGCCGTGTCGCTCCTGGTTTCCATCGTGTGGGAAGAACGGAGTAACGAGTCTCACGCAGAAA
  AAGGTCTTGAGAGCACCTTGTGGCGCACCCAGTGTAACTGTGACGAAGCCAACCATGAAGGACCGCTCTT
  CAACTCCCCCCTTACATGTTCACGTGGATGAGAACACCCCTGTCCACGTCCACATAAAAAAACTCCCGAA
  ACCATCAGCGACCAGCAGCCAGAAATCTCACAAGCGAGGAATGAAAGGGGACACTGTGAATGTGCGGCGG
  AGTGTCCGGGTGAAAACCAAGAATCCACCTCATTGCCTGGAGATCACGCCACCATCTTCAGAAAAGCTGG
  TCTCAGTGATGCGGTTAAGTGACCTCTCTACAGAAGATGATGACTCAGGTCACTGTAAAATGAACCGTTA
  TGATAAGAAGATTGATAGTCTAATGAATGCGGTTGGTTGTCTGAAGTCTGAGGTCAAGATGCAAAAAGGT
  GAGCGCCAGATGGCCAAAAGGTTCCTGGAGGAACGGAAGGAAGAGCTGGAGGAGGTGGCCCACGAACTGG
  CTGAGACTGAGCACGAGAACACGGTGTTGAGGCACAACATCGAGCGCATGAAGGAGGAGAAGGACTTCAC
  CATACTTCAGAAGAAACACCTACAACAGGAGAAGGAGTGCCTCATGTCCAAGCTGGTGGAGGCGGAAATG
  GATGGGGCTGCGGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGAAGCTGAAAACGGAGAAAC
  AAATGACCTGCACGGACATCAACACCCTGACAAGGCAGAAGGAACTTCTCCTGCAGAAGCTGAGCACATT
  TGAGGAGACCAACCGCACCCTCCGAGACCTCCTGAGGGAACAGCACTGCAAAGAGGATTCTGAAAGACTA
  ATGGAGCAACAAGGAGCACTGCTGAAACGGCTGGCGGAGGCCGACTCAGAGAAAGCGCGCCTGCTGTTAC
  TGCTGCAAGACAAGGACAAGGAGGTGGAAGAGCTCCTTCAGGAAATACAATGTGAGAAGGCTCAAGCAAA
  GACAGCCTCTGAGCTTTCTAAATCCATGGAGTCCATGCGTGGGCATTTGCAGGCACAGCTTCGGTCCAAA
  GAGGCTGAGAACAGTCGCCTGTGCATGCAGATTAAGAATCTGGAGCGCAGCGGGAATCAGCATAAGGCAG
  AAGTGGAGGCCATCATGGAGCAGCTGAAGGAGTTGAAGCAGAAGGGAGACCGAGACAAAGAGAGCTTGAA
  GAAGGCCATCCGAGCCCAGAAGGAGCGAGCCGAGAAGAGCGAGGAGTATGCTGAGCAGCTACACGTGCAA
  CTCGCTGACAAGGATCTTTATGTCGCTGAAGCTTTATCCACTCTGGAATCCTGGAGGAGCCGCTACAACC
  AAGTTGTAAAAGAAAAGGGAGACCTTGAGCTGGAAATTATTGTCCTGAATGACCGGGTAACAGATCTTGT
  AAACCAACAACAAACCCTGGAGGAGAAGATGCGGGAAGACCGGGATAGCCTGGTGGAGAGACTACACCGT
  CAGACTGCTGAGTATTCCGCATTCAAGCTGGAGAATGAGAGGCTGAAGGCCAGCTTTGCTCCAATGGAGG
  ACAAACTCAACCAGGCACACCTCGAGGTCCAGCAGCTGAAGGCCTCAGTGAAGAACTATGAGGGGATGAT
  TGACAACTATAAGAGTCAGGTGATGAAGACCAGATTGGAGGCTGATGAAGTAGCTGCCCAGCTAGAACGC
  TGTGACAAAGAGAACAAGATCCTTAAAGATGAGATGAACAAAGAGATTGAGGCGGCACGAAGGCAGTTCC
  AGTCTCAGCTGGCTGACCTGCAGCAGCTCCCTGACATCCTGAAGATCACGGAGGCGAAGCTGGCTGAGTG
  CCAAGACCAACTGCAGGGCTATGAGCGGAAGAACATCGACCTCACAGCCATCATATCAGACCTGCGCAGC
  CGGATCGAACACCAGGGGGACAAGCTGGAGATGGCGAGAGAGAAACATCAGGCTTCCCAGAAGGAAAATA
  AACAGCTGAGTCTGAAGGTGGATGAACTGGAGAGGAAACTGGAGGCGACCAGTGCCCAGAATATCGAGTT
  CCTACAGGTGATTGCCAAGAGGGAGGAGGCAATCCACCAGTCCCAGCTGCGGCTGGAGGAGAAAACACGG
  GAATGTGGGACCCTGGCAAGGCAGTTGGAGAGTGCCATTGAAGATGCGAGGAGGCAGGTGGAACAAACCA
  AGGAGCACGCACTCTCCAAGGAGCGAGCAGCCCAGAACAAAATCCTGGACCTTGAGACCCAGCTGAGCAG
  AACCAAAACGGAATTGAGCCAGCTGCGGCGGAGCCGTGATGATGCGGACCGCCGCTACCAGAGCCGGCTG
  CAAGACCTGAAAGATCGCCTGGAGCAGTCCGAGAGCACCAACCGCAGCATGCAGAACTACGTCCAGTTCC
  TCAAATCATCATACGCCAACGTGTTTGGGGATGGTCCCTATTCCACCTTCCTGACTAGCTCTCCCATCCG
  CTCCCGATCTCCTCCTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231435 representing NM_153435
  Red=Cloning site Green=Tags(s)
  
  MGELPTGCRKRRRKRGGAAARARLHPPRRLHGTTLASDFNDFIRRRFWAQPCRSWFPSCGKNGVTSLTQK
  KVLRAPCGAPSVTVTKPTMKDRSSTPPLHVHVDENTPVHVHIKKLPKPSATSSQKSHKRGMKGDTVNVRR
  SVRVKTKNPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSEVKMQKG
  ERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERMKEEKDFTILQKKHLQQEKECLMSKLVEAEM
  DGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCKEDSERL
  MEQQGALLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHLQAQLRSK
  EAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKESLKKAIRAQKERAEKSEEYAEQLHVQ
  LADKDLYVAEALSTLESWRSRYNQVVKEKGDLELEIIVLNDRVTDLVNQQQTLEEKMREDRDSLVERLHR
  QTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLER
  CDKENKILKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLRS
  RIEHQGDKLEMAREKHQASQKENKQLSLKVDELERKLEATSAQNIEFLQVIAKREEAIHQSQLRLEEKTR
  ECGTLARQLESAIEDARRQVEQTKEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRL
  QDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPPA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153435
• ORF Size: 2679 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153435.1, NP_702913.1
• RefSeq Size: 3805 bp
• RefSeq ORF: 2682 bp
• Locus ID: 4957
• UniProt ID: Q5BJF6
• Cytogenetics: 9q34.11
• Gene Summary: The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010]