BCAR1 (NM_001170716) Human Tagged ORF Clone

CAT#: RG230561

BCAR1 (tGFP-tagged) - Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 3

Product Images

Circular map for RG230561

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BCAR1
• Synonyms: CAS; CAS1; CASS1; CRKAS; P130Cas
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230561 representing NM_001170716
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCACCCACCGTCCCCAGGAAGCAGAACAGAGGGGTCGGACCCCTGGTCCTTCCTTTGAGTGGAACG
  TGCTGGCCAAAGCGCTCTATGACAATGTGGCCGAGTCCCCGGATGAGCTCTCCTTCCGCAAGGGTGACAT
  CATGACGGTGCTGGAGCAGGACACGCAGGGCCTGGACGGCTGGTGGCTCTGCTCGCTGCATGGGCGCCAG
  GGCATCGTGCCTGGGAACCGCCTCAAGATCTTGGTGGGCATGTATGATAAGAAGCCAGCAGGGCCTGGCC
  CCGGCCCTCCCGCCACCCCGGCCCAGCCTCAGCCTGGCCTCCATGCCCCAGCGCCTCCGGCCTCCCAGTA
  CACGCCCATGCTCCCCAACACCTACCAGCCCCAGCCAGACAGCGTCTACCTGGTGCCCACTCCCAGCAAG
  GCTCAGCAAGGCCTCTACCAAGTCCCGGGTCCCAGCCCTCAGTTCCAGTCTCCCCCAGCCAAGCAGACAT
  CCACCTTCTCGAAGCAGACACCCCATCACCCGTTTCCCAGCCCGGCCACAGACCTGTACCAGGTGCCCCC
  AGGGCCTGGAGGCCCTGCCCAGGATATTTACCAGGTGCCACCTTCTGCCGGGATGGGGCATGACATCTAC
  CAGGTCCCCCCGTCCATGGACACACGCAGCTGGGAGGGCACGAAGCCCCCGGCAAAGGTGGTGGTGCCCA
  CCCGCGTGGGGCAGGGCTATGTATACGAGGCCGCCCAGCCGGAGCAGGACGAGTACGACATCCCGCGACA
  CCTGCTGGCCCCGGGGCCACAGGACATCTATGATGTGCCCCCGGTTCGGGGGCTGCTTCCCAGCCAGTAT
  GGCCAGGAGGTGTATGACACACCCCCCATGGCTGTCAAGGGTCCCAATGGCCGAGACCCGTTGCTGGAGG
  TGTATGACGTGCCCCCCAGTGTGGAGAAGGGCCTGCCACCGTCCAACCACCACGCAGTCTACGACGTTCC
  TCCATCGGTGAGCAAGGATGTGCCCGATGGCCCACTGCTGCGTGAGGAGACCTACGATGTGCCCCCCGCC
  TTCGCCAAGGCCAAGCCCTTTGACCCGGCCCGCACCCCACTGGTACTGGCTGCGCCCCCTCCAGACTCCC
  CGCCGGCCGAGGACGTGTATGACGTGCCGCCCCCGGCTCCTGACCTCTACGACGTGCCCCCTGGCTTGCG
  GCGGCCTGGCCCGGGCACCCTGTACGATGTGCCCCGTGAACGGGTGCTTCCTCCTGAGGTGGCTGATGGT
  GGCGTGGTCGACAGTGGTGTGTATGCGGTGCCTCCCCCAGCTGAACGTGAAGCCCCGGCAGAGGGCAAGC
  GCCTGTCGGCCTCCAGCACCGGCAGCACACGCAGCAGCCAGTCTGCGTCCTCCTTGGAGGTGGCAGGGCC
  GGGCCGGGAACCCCTGGAGCTGGAAGTTGCTGTGGAGGCCCTGGCACGGCTGCAGCAGGGTGTGAGCGCC
  ACCGTTGCCCACCTTCTGGACCTGGCAGGCAGCGCCGGTGCGACTGGGAGCTGGCGTAGCCCCTCTGAGC
  CACAGGAGCCGCTGGTGCAGGACCTGCAGGCTGCTGTGGCCGCTGTCCAGAGTGCCGTCCACGAGCTGTT
  GGAGTTTGCCCGCAGCGCGGTGGGCAATGCTGCCCACACATCTGACCGTGCCCTGCATGCCAAGCTTAGC
  CGGCAGCTGCAGAAGATGGAGGACGTGCACCAGACGCTGGTGGCACATGGTCAGGCCCTCGACGCTGGCC
  GGGGAGGCTCTGGAGCCACCCTTGAGGACCTGGACCGGCTGGTGGCCTGCTCGCGGGCTGTGCCCGAGGA
  CGCCAAGCAGCTGGCCTCCTTCCTGCACGGCAATGCCTCACTGCTCTTCAGACGGACCAAGGCCACTGCC
  CCGGGGCCTGAGGGGGGTGGCACCCTGCACCCCAACCCCACTGACAAGACCAGCAGCATCCAGTCACGAC
  CCCTGCCCTCACCCCCTAAGTTCACCTCCCAGGACTCGCCAGATGGGCAGTACGAGAACAGCGAGGGGGG
  CTGGATGGAGGACTATGACTACGTCCACCTACAGGGGAAGGAGGAGTTTGAGAAGACCCAGAAGGAGCTG
  CTGGAAAAGGGCAGCATCACGCGGCAGGGCAAGAGCCAGCTGGAGTTGCAGCAGCTGAAGCAGTTTGAAC
  GACTGGAACAGGAGGTGTCACGGCCCATAGACCACGACCTGGCCAACTGGACGCCAGCCCAACCCCTGGC
  CCCGGGGCGAACAGGCGGCCTGGGGCCCTCGGACCGGCAGCTGCTGCTCTTCTACCTGGAGCAGTGTGAG
  GCCAACCTGACCACACTGACCAACGCCGTGGACGCCTTCTTTACCGCCGTGGCCACCAACCAGCCGCCCA
  AGATCTTTGTGGCGCACAGCAAGTTCGTCATCCTCAGCGCCCACAAGCTGGTGTTCATCGGGGACACACT
  GTCACGGCAGGCCAAGGCTGCTGACGTGCGCAGCCAGGTGACCCACTACAGCAACCTGCTGTGCGACCTC
  CTGCGCGGCATCGTGGCCACCACCAAGGCCGCTGCCTTGCAGTACCCATCGCCTTCCGCGGCCCAGGACA
  TGGTGGAGAGGGTCAAGGAGCTGGGCCACAGCACCCAGCAGTTCCGCCGCGTCCTAGGCCAGCTGGCAGC
  CGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230561 representing NM_001170716
  Red=Cloning site Green=Tags(s)
  
  MLTHRPQEAEQRGRTPGPSFEWNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQ
  GIVPGNRLKILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSK
  AQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIY
  QVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQY
  GQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPA
  FAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADG
  GVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSA
  TVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLS
  RQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATA
  PGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKEL
  LEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCE
  ANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDL
  LRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVLGQLAAA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170716
• ORF Size: 2664 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170716.3
• RefSeq Size: 3195 bp
• RefSeq ORF: 2667 bp
• Locus ID: 9564
• UniProt ID: P56945
• Cytogenetics: 16q23.1
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]