TENT5D (NM_001170574) Human Tagged ORF Clone

CAT#: RG229980

FAM46D (tGFP-tagged) - Human family with sequence similarity 46, member D (FAM46D), transcript variant 1

Product Images

Circular map for RG229980

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TENT5D
• Synonyms: CT1.26; CT112; FAM46D
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229980 representing NM_001170574
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAAATCAGATTCACCAATCTCACTTGGGATCAAGTTATAACACTGGATCAAGTGTTAGATGAAG
  TAATTCCAATTCATGGAAAGGGGAATTTCCCCACAATGGAGGTAAAACCAAAAGACATCATTCATGTTGT
  GAAAGATCAACTCATAGGGCAAGGAATTATTGTTAAAGATGCCAGATTGAATGGTTCCGTAGCAAGTTAC
  ATACTTGCAAGCCACAATGGAATCAGCTATAAGGATCTGGACGTTATTTTTGGTGTTGAGCTTCCAGGTA
  ACGAAGAATTTCAGGTTGTTAAAGATGCAGTTCTAGACTGTCTACTTGACTTTTTACCAAAAGATGTAAA
  GAAGGAAAAGCTCTCCCCAGATATCATGAAAGACGCTTACGTACAGAAATTGGTCAAGGTTTGCAATGGG
  CATGATTGTTGGAGTCTTATCTCCCTTTCAAATAACACTGGGAAGAATTTAGAACTAAAATTTGTGAGTT
  CACTCAGACGGCAGTTTGAATTTAGTGTAGATTCCTTTCAAATTGTTTTGGATCCCATGTTAGACTTCTA
  CAGTGACAAAAATGCCAAGCTAACCAAAGAATCCTATCCTGTTGTGGTAGCTGAAAGCATGTATGGAGAC
  TTCCAGGAAGCAATGACACATTTGCAACACAAGCTCATATGTACCAGGAAACCTGAAGAGATTAGAGGTG
  GTGGCCTTCTGAAGTACTGCAGCTTGCTGGTTCATGGCTTCAAGCCAGCCTGTATGTCAGAAATCAAAAA
  CCTAGAACGTTATATGTGCTCTAGATTCTTTATTGATTTTCCTCATATAGAAGAACAGCAAAAGAAAATT
  GAATCATACCTCCACAACCATTTCATAGGTGAAGGAATGACCAAGTATGACTACCTTATGACCTTGCATG
  GAGTTGTGAATGAAAGCACTGTTTGCCTCATGAGTTATGAAAGAAGACAGATTCTCCACCTGATCACCAT
  GATGGCTTTGAAAGTACTTGGAGAACTAAATATTCTACCCAATACACAAAAGGTAACTTGCTTTTATCAG
  CCTGCTCCGTACTTTGCAGCTGAGGCAAGGTACCCTATTTATGTAATACCTGAGCCACCCCCCGTTAGCT
  TCCAGCCATACCACCCACTGCACTTTCGTGGATCAAATGGTATGAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229980 representing NM_001170574
  Red=Cloning site Green=Tags(s)
  
  MSEIRFTNLTWDQVITLDQVLDEVIPIHGKGNFPTMEVKPKDIIHVVKDQLIGQGIIVKDARLNGSVASY
  ILASHNGISYKDLDVIFGVELPGNEEFQVVKDAVLDCLLDFLPKDVKKEKLSPDIMKDAYVQKLVKVCNG
  HDCWSLISLSNNTGKNLELKFVSSLRRQFEFSVDSFQIVLDPMLDFYSDKNAKLTKESYPVVVAESMYGD
  FQEAMTHLQHKLICTRKPEEIRGGGLLKYCSLLVHGFKPACMSEIKNLERYMCSRFFIDFPHIEEQQKKI
  ESYLHNHFIGEGMTKYDYLMTLHGVVNESTVCLMSYERRQILHLITMMALKVLGELNILPNTQKVTCFYQ
  PAPYFAAEARYPIYVIPEPPPVSFQPYHPLHFRGSNGMS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170574
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170574.1, NP_001164045.1
• RefSeq Size: 3106 bp
• RefSeq ORF: 1170 bp
• Locus ID: 169966
• UniProt ID: Q8NEK8
• Cytogenetics: Xq21.1
• Gene Summary: Antibodies against the protein encoded by this gene were found only in plasma from cancer patients. While it may be a target for immunotherapy, the function of this gene is unknown. [provided by RefSeq, Dec 2009]