LENG4 (MBOAT7) (NM_001146083) Human Tagged ORF Clone

CAT#: RG228875

MBOAT7 (tGFP-tagged) - Human membrane bound O-acyltransferase domain containing 7 (MBOAT7), transcript variant 3

Product Images

Circular map for RG228875

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LENG4
• Synonyms: BB1; hMBOA-7; LENG4; LPIAT; LPLAT; LRC4; MBOA7; MRT57; OACT7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228875 representing NM_001146083
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGCAGCCGCTGTGGGCCTGGGGCTCACCCTGTTCACCTGTGGCCCCCACACTTTGCATTCTCTG
  GTCACCATCCTCGGGACCTGGGCCCTCATTCAGGCCCAGCCCTGCTGGTGAGCCTGGCCAGTGAAGTCCA
  GGACCTGCATCTGGCCCAGAGGAAGGAAATGGCCTCAGGCTTCAGCAAGGGGCCCACCCTGGGGCTGCTG
  CCCGACGTGCCCTCCCTGATGGAGACACTCAGCTACAGCTACTGCTACGTGGGAATCATGACAGGCCCGT
  TCTTCCGCTACCGCACCTACCTGGACTGGCTGGAGCAGCCCTTCCCCGGGGCAGTGCCCAGCCTGCGGCC
  CCTGCTGCGCCGCGCCTGGCCGGCCCCGCTCTTCGGCCTGCTGTTCCTGCTCTCCTCTCACCTCTTCCCG
  CTGGAGGCCGTGCGCGAGGACGCCTTCTACGCCCGCCCGCTGCCCGCCCGCCTCTTCTACATGATCCCCG
  TCTTCTTCGCCTTCCGCATGCGCTTCTACGTGGCCTGGATTGCCGCCGAGTGCGGCTGCATTGCCGCCGG
  CTTTGGGGCCTACCCCGTGGCCGCCAAAGCCCGGGCCGGAGGCGGCCCCACCCTCCAATGCCCACCCCCC
  AGCAGTCCGGAGAAGGCGGCTTCCTTGGAGTATGACTATGAGACCATCCGCAACATCGACTGCTACAGCA
  CAGATTTCTGCGTGCGGGTGCGCGATGGCATGCGGTACTGGAACATGACGGTGCAGTGGTGGCTGGCGCA
  GTATATCTACAAGAGCGCACCTGCCCGTTCCTATGTCCTGCGGAGCGCCTGGACCATGCTGCTGAGCGCC
  TACTGGCACGGCCTCCACCCGGGCTACTACCTGAGCTTCCTGACCATCCCGCTGTGCCTGGCTGCCGAGG
  GCCGGCTGGAGTCAGCCCTGCGGGGGCGGCTGAGCCCAGGGGGCCAGAAGGCCTGGGACTGGGTGCACTG
  GTTCCTGAAGATGCGCGCCTATGACTACATGTGCATGGGCTTCGTGCTGCTCTCCTTGGCCGACACCCTT
  CGGTACTGGGCCTCCATCTACTTCTGTATCCACTTCCTGGCCCTGGCAGCCCTGGGGCTGGGGCTGGCTT
  TAGGTGGGGGCAGCCCCAGCCGGCGGAAGGCAGCATCCCAGCCCACCAGCCTTGCCCCGGAGAAGCTCCG
  GGAGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228875 representing NM_001146083
  Red=Cloning site Green=Tags(s)
  
  MGSSRCGPGAHPVHLWPPHFAFSGHHPRDLGPHSGPALLVSLASEVQDLHLAQRKEMASGFSKGPTLGLL
  PDVPSLMETLSYSYCYVGIMTGPFFRYRTYLDWLEQPFPGAVPSLRPLLRRAWPAPLFGLLFLLSSHLFP
  LEAVREDAFYARPLPARLFYMIPVFFAFRMRFYVAWIAAECGCIAAGFGAYPVAAKARAGGGPTLQCPPP
  SSPEKAASLEYDYETIRNIDCYSTDFCVRVRDGMRYWNMTVQWWLAQYIYKSAPARSYVLRSAWTMLLSA
  YWHGLHPGYYLSFLTIPLCLAAEGRLESALRGRLSPGGQKAWDWVHWFLKMRAYDYMCMGFVLLSLADTL
  RYWASIYFCIHFLALAALGLGLALGGGSPSRRKAASQPTSLAPEKLREE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146083
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146083.2
• RefSeq Size: 2488 bp
• RefSeq ORF: 1200 bp
• Locus ID: 79143
• UniProt ID: Q96N66
• Cytogenetics: 19q13.42
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the membrane-bound O-acyltransferases family of integral membrane proteins that have acyltransferase activity. The encoded protein is a lysophosphatidylinositol acyltransferase that has specificity for arachidonoyl-CoA as an acyl donor. This protein is involved in the reacylation of phospholipids as part of the phospholipid remodeling pathway known as the Land cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]