RAB7B (NM_001164522) Human Tagged ORF Clone
CAT#: RG228755
- TrueORF®
RAB7B (tGFP-tagged) - Human RAB7B, member RAS oncogene family (RAB7B), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001164522" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | RAB7B |
Synonyms | RAB7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228755 representing NM_001164522
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCCCGGAAGAAGGTGGACCTGAAACTCATTATCGTCGGAGCCATTGGTGTGGGAAAGACCTCCC TCCTTCACCAATATGTGCACAAGACGTTTTATGAGGAATACCAGACCACACTGGGGGCCAGCATCCTCTC CAAGATTATCATATTGGGTGACACAACTTTGAAGTTACAGATCTGGGACACGGGCGGTCAGGAGCGGTTC CGCTCCATGGTGTCCACGTTCTACAAGGGCTCCGATGGCTGCATCCTAGCTTTTGATGTCACCGACCTGG AGTCTTTTGAAGCCCTGGATATCTGGCGGGGTGATGTCCTGGCCAAGATTGTCCCCATGGAGCAGTCCTA CCCCATGGTGTTGTTGGGGAACAAGATCGATCTGGCAGACCGGAAGGTACCCCAGGAAGTAGCTCAAGGC TGGTGTAGAGAGAAAGATATTCCTTACTTTGAAGTCAGTGCCAAGAATGACATCAATGTGGTGCAAGCGT TTGAGATGCTGGCCAGTAGGGCTCTGTCGAGGTACCAGAGCATCTTAGAAAATCACCTCACAGAATCCAT CAAGCTCTCGCCAGACCAGTCAAGGAGCAGATGCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228755 representing NM_001164522
Red=Cloning site Green=Tags(s) MNPRKKVDLKLIIVGAIGVGKTSLLHQYVHKTFYEEYQTTLGASILSKIIILGDTTLKLQIWDTGGQERF RSMVSTFYKGSDGCILAFDVTDLESFEALDIWRGDVLAKIVPMEQSYPMVLLGNKIDLADRKVPQEVAQG WCREKDIPYFEVSAKNDINVVQAFEMLASRALSRYQSILENHLTESIKLSPDQSRSRCC TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001164522 |
ORF Size | 597 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164522.3 |
RefSeq Size | 2958 bp |
RefSeq ORF | 600 bp |
Locus ID | 338382 |
UniProt ID | Q96AH8 |
Cytogenetics | 1q32.1 |
Protein Families | Druggable Genome |
Gene Summary | Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228755 | RAB7B (Myc-DDK-tagged)-Human RAB7B, member RAS oncogene family (RAB7B), transcript variant 2 |
USD 300.00 |
|
RC228755L3 | Lenti ORF clone of Human RAB7B, member RAS oncogene family (RAB7B), transcript variant 2, Myc-DDK-tagged |
USD 600.00 |
|
RC228755L4 | Lenti ORF clone of Human RAB7B, member RAS oncogene family (RAB7B), transcript variant 2, mGFP tagged |
USD 600.00 |
|
SC327390 | RAB7B (untagged)-Human RAB7B member RAS oncogene family (RAB7B) transcript variant 2 |
USD 330.00 |
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