KCNQ5 (NM_001160134) Human Tagged ORF Clone

CAT#: RG228591

KCNQ5 (tGFP-tagged) - Human potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5), transcript variant 5

Product Images

Circular map for RG228591

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNQ5
• Synonyms: Kv7.5; MRD46
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228591 representing NM_001160134
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGCCACCACGCGGGAGGAGAGGAGGGCGGCGCCGCCGGGCTCTGGGTGAAGAGCGGCGCAGCGG
  CGGCGGCGGCGGGCGGGGGGCGCTTGGGCAGCGGCATGAAGGATGTGGAGTCCGGCCGGGGCAGGGTGCT
  GCTGAACTCGGCAGCCGCCAGGGGCGACGGCCTGCTACTGCTGGGCACCCGCGCGGCCACGCTCGGTGGC
  GGCGGCGGTGGCCTGAGGGAGAGCCGCCGGGGCAAGCAGGGGGCCCGGATGAGCCTGCTGGGGAAGCCGC
  TCTCTTACACGAGTAGCCAGAGCTGCCGGCGCAACGTCAAGTACCGGCGGGTGCAGAACTACCTGTACAA
  CGTGCTGGAGAGACCCCGCGGCTGGGCGTTCATCTACCACGCTTTCGTTTTTCTCCTTGTCTTTGGTTGC
  TTGATTTTGTCAGTGTTTTCTACCATCCCTGAGCACACAAAATTGGCCTCAAGTTGCCTCTTGATCCTGG
  AGTTCGTGATGATTGTCGTCTTTGGTTTGGAGTTCATCATTCGAATCTGGTCTGCGGGTTGCTGTTGTCG
  ATATAGAGGATGGCAAGGAAGACTGAGGTTTGCTCGAAAGCCCTTCTGTGTTATAGATACCATTGTTCTT
  ATCGCTTCAATAGCAGTTGTTTCTGCAAAAACTCAGGGTAATATTTTTGCCACGTCTGCACTCAGAAGTC
  TCCGTTTCCTACAGATCCTCCGCATGGTGCGCATGGACCGAAGGGGAGGCACTTGGAAATTACTGGGTTC
  AGTGGTTTATGCTCACAGCAAGGAATTAATCACAGCTTGGTACATAGGATTTTTGGTTCTTATTTTTTCG
  TCTTTCCTTGTCTATCTGGTGGAAAAGGATGCCAATAAAGAGTTTTCTACATATGCAGATGCTCTCTGGT
  GGGGCACAATTACATTGACAACTATTGGCTATGGAGACAAAACTCCCCTAACTTGGCTGGGAAGATTGCT
  TTCTGCAGGCTTTGCACTCCTTGGCATTTCTTTCTTTGCACTTCCTGCCGGCATTCTTGGCTCAGGTTTT
  GCATTAAAAGTACAAGAACAACACCGCCAGAAACACTTTGAGAAAAGAAGGAACCCAGCTGCCAACCTCA
  TTCAGTGTGTTTGGCGTAGTTACGCAGCTGATGAGAAATCTGTTTCCATTGCAACCTGGAAGCCACACTT
  GAAGGCCTTGCACACCTGCAGCCCTACCAAGAAAGAACAAGGGGAAGCATCAAGCAGAATTATGAAATTT
  CATGTTGCAAAACGGAAGTTTAAGGAAACATTACGTCCATATGATGTAAAAGATGTCATTGAACAATATT
  CTGCTGGTCATCTGGACATGTTGTGTAGAATTAAAAGCCTTCAAACACGTGTTGATCAAATTCTTGGAAA
  AGGGCAAATCACATCAGATAAGAAGAGCCGAGAGAAAATAACAGCAGAACATGAGACCACAGACGATCTC
  AGTATGCTCGGTCGGGTGGTCAAGGTTGAAAAACAGGTACAGTCCATAGAATCCAAGCTGGACTGCCTAC
  TAGACATCTATCAACAGGTCCTTCGGAAAGGCTCTGCCTCAGCCCTCGCTTTGGCTTCATTCCAGATCCC
  ACCTTTTGAATGTGAACAGACATCTGACTATCAAAGCCCTGTGGATAGCAAAGATCTTTCGGGTTCCGCA
  CAAAACAGTGGCTGCTTATCCAGATCAACTAGTGCCAACATCTCGAGAGGCCTGCAGTTCATTCTGACGC
  CAAATGAGTTCAGTGCCCAGACTTTCTACGCGCTTAGCCCTACTATGCACAGTCAAGCAACACAGGTGCC
  AATTAGTCAAAGCGATGGCTCAGCAGTGGCAGCCACCAACACCATTGCAAACCAAATAAATACGGCACCC
  AAGCCAGCAGCCCCAACAACTTTACAGATCCCACCTCCTCTCCCAGCCATCAAGCATCTGCCCAGGCCAG
  AAACTCTGCACCCTAACCCTGCAGGCTTACAGGAAAGCATTTCTGACGTCACCACCTGCCTTGTTGCCTC
  CAAGGAAAATGTTCAGGTTGCACAGTCAAATCTCACCAAGGACCGTTCTATGAGGAAAAGCTTTGACATG
  GGAGGAGAAACTCTGTTGTCTGTCTGTCCCATGGTGCCGAAGGACTTGGGCAAATCTTTGTCTGTGCAAA
  ACCTGATCAGGTCGACCGAGGAACTGAATATACAACTTTCAGGGAGTGAGTCAAGTGGCTCCAGAGGCAG
  CCAAGATTTTTACCCCAAATGGAGGGAATCCAAATTGTTTATAACTGATGAAGAGGTGGGTCCCGAAGAG
  ACAGAGACAGACACTTTTGATGCCGCACCGCAGCCTGCCAGGGAAGCTGCCTTTGCATCAGACTCTCTAA
  GGACTGGAAGGTCACGATCATCTCAGAGCATTTGTAAGGCAGGAGAAAGTACAGATGCCCTCAGCTTGCC
  TCATGTCAAACTGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228591 representing NM_001160134
  Red=Cloning site Green=Tags(s)
  
  MPRHHAGGEEGGAAGLWVKSGAAAAAAGGGRLGSGMKDVESGRGRVLLNSAAARGDGLLLLGTRAATLGG
  GGGGLRESRRGKQGARMSLLGKPLSYTSSQSCRRNVKYRRVQNYLYNVLERPRGWAFIYHAFVFLLVFGC
  LILSVFSTIPEHTKLASSCLLILEFVMIVVFGLEFIIRIWSAGCCCRYRGWQGRLRFARKPFCVIDTIVL
  IASIAVVSAKTQGNIFATSALRSLRFLQILRMVRMDRRGGTWKLLGSVVYAHSKELITAWYIGFLVLIFS
  SFLVYLVEKDANKEFSTYADALWWGTITLTTIGYGDKTPLTWLGRLLSAGFALLGISFFALPAGILGSGF
  ALKVQEQHRQKHFEKRRNPAANLIQCVWRSYAADEKSVSIATWKPHLKALHTCSPTKKEQGEASSRIMKF
  HVAKRKFKETLRPYDVKDVIEQYSAGHLDMLCRIKSLQTRVDQILGKGQITSDKKSREKITAEHETTDDL
  SMLGRVVKVEKQVQSIESKLDCLLDIYQQVLRKGSASALALASFQIPPFECEQTSDYQSPVDSKDLSGSA
  QNSGCLSRSTSANISRGLQFILTPNEFSAQTFYALSPTMHSQATQVPISQSDGSAVAATNTIANQINTAP
  KPAAPTTLQIPPPLPAIKHLPRPETLHPNPAGLQESISDVTTCLVASKENVQVAQSNLTKDRSMRKSFDM
  GGETLLSVCPMVPKDLGKSLSVQNLIRSTEELNIQLSGSESSGSRGSQDFYPKWRESKLFITDEEVGPEE
  TETDTFDAAPQPAREAAFASDSLRTGRSRSSQSICKAGESTDALSLPHVKLK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160134
• ORF Size: 2466 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160134.2
• RefSeq Size: 6265 bp
• RefSeq ORF: 2469 bp
• Locus ID: 56479
• UniProt ID: Q9NR82
• Cytogenetics: 6q13
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: This gene is a member of the KCNQ potassium channel gene family that is differentially expressed in subregions of the brain and in skeletal muscle. The protein encoded by this gene yields currents that activate slowly with depolarization and can form heteromeric channels with the protein encoded by the KCNQ3 gene. Currents expressed from this protein have voltage dependences and inhibitor sensitivities in common with M-currents. They are also inhibited by M1 muscarinic receptor activation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]