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SDK2 (NM_001144952) Human Tagged ORF Clone
CAT#: RG227752
- TrueORF®
SDK2 (tGFP-tagged) - Human sidekick homolog 2 (chicken) (SDK2)
ORF Plasmid: DDK
"NM_001144952" in other vectors (2)
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Frequently bought together (4)
Other products for "SDK2"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | SDK2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227752 representing NM_001144952
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGGCTTTTGATCTGGACACTGCTAGCTCTGCATCAGATCCGCGCGGCCAGAGCCCAAGATGATG TGTCCCCGTATTTCAAGACAGAGCCTGTGCGGACACAGGTGCACTTGGAAGGAAACCGCCTGGTGCTTAC ATGCATGGCCGAGGGCAGCTGGCCACTGGAGTTCAAGTGGCTCCACAACAACAGGGAGCTGACCAAGTTC TCCCTGGAATACAGATACATGATCACCAGCCTGGACCGCACCCACGCTGGCTTTTACCGTTGCATCGTGC GGAACCGAATGGGGGCCCTGCTGCAGCGGCAAACCGAGGTCCAGGTGGCCTACATGGGGAGCTTTGAGGA AGGTGAGAAGCACCAGAGCGTCTCCCACGGAGAAGCAGCTGTCATCCGTGCCCCGCGCATCGCCAGCTTC CCCCAGCCACAGGTGACCTGGTTCCGGGACGGCCGCAAGATCCCGCCCAGCAGCCGCATAGCCATCACGC TGGAGAACACCCTTGTCATCCTGTCAACGGTGGCCCCTGACGCAGGTCGCTACTATGTGCAGGCTGTTAA CGACAAAAACGGGGATAACAAGACCAGCCAGCCCATCACGCTCACCGTGGAGAATGTAGGGGGGCCTGCA GACCCCATCGCACCCACCATCATCATCCCACCTAAAAACACCAGCGTGGTGGCCGGCACCTCAGAGGTTA CCTTGGAGTGTGTGGCCAATGCCAGGCCCCTGATCAAGCTACATATCATTTGGAAGAAGGACGGGGTATT GCTGTCGGGCGGCATCAGTGACCACAACCGCCGGCTCACCATCCCCAACCCCACCGGCAGTGACGCCGGC TACTACGAGTGTGAGGCTGTCCTGCGCAGCAGCAGCGTCCCCTCTGTTGTCCGGGGCGCCTACCTCTCAG TGCTGGAACCGCCTCAGTTTGTCAAGGAGCCAGAAAGACACATCACTGCGGAGATGGAGAAGGTGGTGGA CATCCCCTGTCAGGCCAAAGGTGTGCCGCCGCCCTCCATCACCTGGTACAAGGACGCAGCCGTGGTGGAG GTGGAGAAGTTGACCCGCTTCCGGCAGCGCAACGACGGGGGCCTGCAGATCAGCGGCCTGGTGCCCGATG ATACCGGCATGTTCCAGTGCTTCGCCCGCAATGCAGCCGGCGAGGTGCAAACTTCCACCTACCTGGCTGT CACCAGCATCGCCCCTAACATCACCAGAGGCCCCCTGGACAGCACGGTGATCGATGGCATGTCAGTGGTG CTAGCATGTGAGACCTCGGGGGCGCCCCGACCAGCTATCACTTGGCAGAAAGGGGAGCGCATCTTGGCCA GTGGCTCTGTGCAGCTGCCTCGCTTCACACCCCTGGAGTCGGGCAGCCTCCTCATCAGCCCCACACACAT CTCCGATGCGGGGACCTACACCTGCCTGGCCACCAACTCTCGGGGGGTCGATGAGGCCTCAGCAGACCTA GTCGTTTGGGCTCGGACCCGCATCACCAAGCCCCCCCAGGATCAGAGTGTCATCAAGGGCACCCAGGCCT CCATGGTGTGCGGAGTGACCCACGACCCCCGAGTAACCATCAGGTACATCTGGGAGAAGGACGGGGCCAC CCTGGGCACGGAGAGCCATCCTCGTATCCGCCTGGACAGAAACGGCTCCCTGCACATCTCACAGACGTGG TCGGGAGACATCGGCACGTACACCTGCCGGGTGATCTCAGCAGGAGGCAACGACTCTCGCAGTGCCCACC TGCGAGTCAGGCAACTGCCCCACGCGCCCGAGCACCCAGTGGCCACTCTCAGCACCGTGGAAAGGCGAGC CATCAACCTGACGTGGACCAAGCCCTTTGATGGCAACAGCCCCCTGATCCGCTACATTCTGGAGATGTCG GAGAACAATGCCCCCTGGACTGTACTCCTGGCCAGTGTGGACCCCAAAGCTACCTCAGTGACAGTCAAGG GCCTGGTTCCTGCACGCTCCTACCAGTTCCGTCTTTGTGCCGTCAACGACGTGGGGAAAGGACAGTTCAG CAAAGACACCGAGAGGGTCTCCCTCCCCGAGGAGCCCCCCACGGCCCCTCCACAGAACGTCATCGCCAGC GGTCGAACCAACCAGTCCATCATGATCCAGTGGCAGCCGCCTCCTGAGAGCCACCAGAATGGAATTCTCA AGGGTTACATCATCAGGTACTGCCTGGCCGGGCTGCCCGTGGGGTACCAGTTTAAGAACATCACGGATGC TGATGTGAACAACCTGCTGCTGGAGGATCTCATCATTTGGACCAACTACGAGATCGAGGTGGCTGCTTAC AACAGCGCTGGGCTGGGGGTCTACAGCAGTAAAGTCACCGAGTGGACGCTGCAGGGAGTTCCCACGGTCC CTCCGGGCAATGTGCACGCGGAAGCCACCAATTCCACGACCATCCGCTTCACCTGGAACGCCCCCAGCCC CCAGTTCATCAACGGCATCAACCAGGGCTACAAGCTGATCGCCTGGGAGCCGGAACAGGAAGAGGAGGTT ACCATGGTGACCGCCCGGCCTAACTTTCAAGACAGCATCCACGTGGGCTTCGTGTCTGGCCTGAAGAAGT TCACCGAGTACTTCACCTCAGTGCTGTGTTTCACCACCCCCGGGGACGGGCCACGCAGCACCCCGCAGCT GGTGCGCACCCATGAGGATGTGCCTGGGCCCGTGGGACACCTGAGCTTCAGTGAGATCCTGGACACATCG CTGAAGGTCAGCTGGCAAGAGCCGGGAGAGAAAAATGGCATCCTCACAGGGTACCGGATCTCCTGGGAGG AGTACAATCGAACCAACACCCGTGTGACCCACTACCTGCCCAACGTGACCCTGGAGTACCGTGTCACGGG CCTCACCGCGCTCACCACCTACACCATCGAGGTGGCCGCCATGACCTCAAAGGGCCAGGGCCAAGTGTCC GCCTCCACCATCTCCTCTGGGGTGCCCCCAGAACTCCCAGGGCCCCCCACCAACCTGGGCATTTCCAACA TCGGCCCCCGCTCTGTGACCTTGCAGTTCAGGCCAGGCTACGATGGGAAAACCTCCATCTCCCGCTGGCT GGTGGAAGCCCAGGTAGGCGTGGTTGGGGAGGGAGAGGAGTGGTTGCTGATCCACCAGCTCTCCAATGAG CCCGATGCCCGCTCCATGGAGGTGCCCGACCTCAACCCCTTCACCTGCTACAGCTTCCGCATGCGCCAGG TGAACATCGTGGGCACCAGCCCCCCCAGTCAGCCTTCTAGAAAGATCCAGACCCTGCAGGCACCCCCTGA CATGGCCCCAGCCAATGTGTCTCTGCGCACAGCCAGTGAGACCAGCCTGTGGCTGCGCTGGATGCCTCTC CCGGAGATGGAATACAATGGGAACCCTGAGTCCGTGGGCTATAAGATCAAGTACAGCCGGTCAGACGGGC ATGGCAAGACGCTGAGCCACGTGGTGCAGGACCGTGTGGAGCGGGACTACACCATCGAGGACCTGGAGGA GTGGACAGAGTACCGCGTCCAGGTCCAGGCCTTCAACGCCATCGGGAGCGGGCCCTGGAGCCAGACGGTG GTGGGCAGGACCCGGGAGTCAGTTCCCTCTTCCGGCCCCACCAATGTGTCTGCACTGGCCACCACCTCCA GCAGCATGCTGGTGCGCTGGAGCGAGGTCCCCGAGGCTGATCGCAACGGGCTCGTGCTGGGCTATAAGGT GATGTATAAGGAGAAGGACTCGGACACCCAGCCCCGATTCTGGCTGGTGGAAGGCAACTCGTCTCGCAGT GCCCAGCTCACCGGCTTGGGCAAATACGTGCTCTATGAAGTCCAGGTGCTGGCCTTCACACGCATCGGGG ACGGCAGCCCCAGCCACCCTCCCATCCTGGAGCGGACGCTGGATGATGTCCCAGGACCACCCATGGGCAT CCTGTTCCCAGAGGTGCGGACCACGTCTGTGCGGCTGATCTGGCAGCCCCCTGCCGCCCCCAATGGCATC ATTCTTGCTTACCAGATCACACACCGGCTCAACACCACCACGGCCAACACCGCCACTGTGGAGGTGCTGG CACCCAGCGCCCGGCAGTACACAGCCACGGGCCTCAAGCCAGAGTCTGTCTACCTGTTCCGCATCACGGC CCAGACCCGCAAGGGCTGGGGAGAAGCTGCCGAGGCCTTGGTGGTGACCACCGAGAAGAGAGACCGTCCG CAGCCCCCCAGCAGGCCGATGGTGCAGCAGGAGGATGTGAGAGCACGCAGCGTGCTGCTGTCCTGGGAGC CAGGGAGCGACGGGCTCTCCCCTGTGCGCTACTACACCATCCAGACCCGCGAGCTGCCCAGCGGCAGGTG GGCACTGCACTCGGCCTCCGTGAGCCACAATGCCTCCAGCTTCATTGTGGACAGGCTGAAGCCCTTCACG TCCTACAAGTTCCGAGTGAAGGCGACCAATGACATTGGCGACAGCGAGTTCAGCGAGGAGTCGGAGTCAC TGACCACCCTGCAGGCTGCCCCCGATGAAGCACCCACCATCCTCTCCGTGACGCCCCACACCACCACCTC CGTGCTAATCCGATGGCAGCCGCCAGCAGAGGACAAGATCAATGGCATCCTCCTGGGCTTCCGGATCCGA TACCGGGAGCTGCTCTATGAAGGACTGAGGGGCTTCACGCTTCGAGGCATCAACAACCCAGGGGCCACAT GGGCTGAGCTTACCTCCATGTACTCCATGCGGAACCTGAGCCGGCCCAGCCTCACGCAGTACGAGCTGGA CAACCTGAACAAGCACAGGCGGTACGAGATACGGATGAGCGTGTACAACGCTGTGGGTGAGGGGCCCTCC AGCCCCCCGCAGGAGGTCTTTGTTGGGGAGGCAGTGCCCACAGCAGCACCTCGTAACGTGGTCGTCCACG GCGCCACGGCCACACAGCTGGACGTGACTTGGGAGCCACCTCCGCTGGACAGCCAGAATGGAGACATCCA GGGGTACAAGATTTATTTCTGGGAAGCCCAGCGGGGGAACCTCACAGAGCGAGTGAAGACGCTTTTCCTG GCTGAGAACAGCGTGAAGCTCAAGAACTTGACTGGCTACACGGCCTACATGGTCAGCGTGGCCGCCTTCA ACGCCGCTGGGGATGGGCCTCGGAGCACCCCCACCCAAGGCCAGACCCAGCAAGCAGCCCCCAGCGCTCC CAGCTCGGTCAAGTTCAGTGAGCTGACCACAACCTCAGTGAATGTGTCCTGGGAAGCCCCGCAGTTCCCC AATGGCATCCTGGAGGGCTACAGGCTGGTGTACGAGCCCTGCAGCCCCGTGGATGGAGTCAGCAAGATCG TGACCGTGGACGTGAAGGGGAACAGCCCCCTGTGGCTGAAGGTGAAGGACCTGGCGGAGGGGGTGACCTA CAGGTTCCGCATCAGAGCCAAGACCTTCACCTACGGGCCGGAGATCGAAGCCAACGTCACCACGGGCCCC GGAGAAGGTGCCCCAGGACCGCCTGGCGTGCCCATCATCGTGCGGTACAGCTCTGCCATCGCCATTCACT GGTCCAGCGGAGACCCGGGCAAAGGGCCCATCACCCGCTACGTCATCGAGGCCAGACCTTCAGACGAGGG ACTATGGGACATCCTCATCAAAGACATCCCCAAGGAGGTGAGCTCCTACACGTTCAGCATGGACATCCTG AAGCCGGGCGTGAGCTATGACTTCCGGGTCATCGCGGTCAACGACTATGGTTTCGGCACCCCCAGCAGCC CCTCCCAGTCTGTGCCAGCCCAGAAAGCCAACCCCTTCTATGAGGAGTGGTGGTTCTTGGTGGTCATTGC CCTGGTCGGCCTCATCTTCATCCTGCTTCTGGTCTTCGTGCTCATCATCCGGGGCCAGAGCAAGAAGTAC GCCAAGAAGACAGACTCGGGGAACAGTGCCAAGTCTGGAGCCCTAGGCCACAGCGAGATGATGAGCTTGG ATGAAAGCAGCTTCCCTGCCCTGGAACTCAACAACAGGCGGCTCTCCGTCAAGAACTCTTTCTGCCGAAA GAACGGCCTGTACACCAGGTCTCCCCCCAGGCCCAGCCCAGGCAGCCTGCACTACTCGGATGAGGATGTC ACCAAATACAACGACCTCATCCCTGCAGAGAGCAGCAGCCTGACGGAGAAGCCCTCAGAAATCTCCGACT CTCAGGGAAGTGACAGCGAGTACGAGGTCGACTCAAACCACCAGAAGGCCCACTCCTTTGTCAACCACTA CATCAGTGACCCCACATACTACAACTCGTGGCGGCGACAGCAGAAGGGCATCTCGAGGGCACAGGCCTAC AGCTACACGGAGAGCGACTCGGGTGAGCCAGACCACACCACCGTCACCAACAGCACCAGCACCCAGCAGG GCAGCCTCTTTCGGCCCAAGGCCAGTCGGACTCCAACGCCCCAGAACCCCCCTAACCCCCCAAGTCAGCA GAGCACCCTCTACCGTCCCCCCAGCAGCCTGGCCCCAGGCTCCCGGGCTCCCATAGCAGGATTTTCATCA TTTGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227752 representing NM_001144952
Red=Cloning site Green=Tags(s) MWGLLIWTLLALHQIRAARAQDDVSPYFKTEPVRTQVHLEGNRLVLTCMAEGSWPLEFKWLHNNRELTKF SLEYRYMITSLDRTHAGFYRCIVRNRMGALLQRQTEVQVAYMGSFEEGEKHQSVSHGEAAVIRAPRIASF PQPQVTWFRDGRKIPPSSRIAITLENTLVILSTVAPDAGRYYVQAVNDKNGDNKTSQPITLTVENVGGPA DPIAPTIIIPPKNTSVVAGTSEVTLECVANARPLIKLHIIWKKDGVLLSGGISDHNRRLTIPNPTGSDAG YYECEAVLRSSSVPSVVRGAYLSVLEPPQFVKEPERHITAEMEKVVDIPCQAKGVPPPSITWYKDAAVVE VEKLTRFRQRNDGGLQISGLVPDDTGMFQCFARNAAGEVQTSTYLAVTSIAPNITRGPLDSTVIDGMSVV LACETSGAPRPAITWQKGERILASGSVQLPRFTPLESGSLLISPTHISDAGTYTCLATNSRGVDEASADL VVWARTRITKPPQDQSVIKGTQASMVCGVTHDPRVTIRYIWEKDGATLGTESHPRIRLDRNGSLHISQTW SGDIGTYTCRVISAGGNDSRSAHLRVRQLPHAPEHPVATLSTVERRAINLTWTKPFDGNSPLIRYILEMS ENNAPWTVLLASVDPKATSVTVKGLVPARSYQFRLCAVNDVGKGQFSKDTERVSLPEEPPTAPPQNVIAS GRTNQSIMIQWQPPPESHQNGILKGYIIRYCLAGLPVGYQFKNITDADVNNLLLEDLIIWTNYEIEVAAY NSAGLGVYSSKVTEWTLQGVPTVPPGNVHAEATNSTTIRFTWNAPSPQFINGINQGYKLIAWEPEQEEEV TMVTARPNFQDSIHVGFVSGLKKFTEYFTSVLCFTTPGDGPRSTPQLVRTHEDVPGPVGHLSFSEILDTS LKVSWQEPGEKNGILTGYRISWEEYNRTNTRVTHYLPNVTLEYRVTGLTALTTYTIEVAAMTSKGQGQVS ASTISSGVPPELPGPPTNLGISNIGPRSVTLQFRPGYDGKTSISRWLVEAQVGVVGEGEEWLLIHQLSNE PDARSMEVPDLNPFTCYSFRMRQVNIVGTSPPSQPSRKIQTLQAPPDMAPANVSLRTASETSLWLRWMPL PEMEYNGNPESVGYKIKYSRSDGHGKTLSHVVQDRVERDYTIEDLEEWTEYRVQVQAFNAIGSGPWSQTV VGRTRESVPSSGPTNVSALATTSSSMLVRWSEVPEADRNGLVLGYKVMYKEKDSDTQPRFWLVEGNSSRS AQLTGLGKYVLYEVQVLAFTRIGDGSPSHPPILERTLDDVPGPPMGILFPEVRTTSVRLIWQPPAAPNGI ILAYQITHRLNTTTANTATVEVLAPSARQYTATGLKPESVYLFRITAQTRKGWGEAAEALVVTTEKRDRP QPPSRPMVQQEDVRARSVLLSWEPGSDGLSPVRYYTIQTRELPSGRWALHSASVSHNASSFIVDRLKPFT SYKFRVKATNDIGDSEFSEESESLTTLQAAPDEAPTILSVTPHTTTSVLIRWQPPAEDKINGILLGFRIR YRELLYEGLRGFTLRGINNPGATWAELTSMYSMRNLSRPSLTQYELDNLNKHRRYEIRMSVYNAVGEGPS SPPQEVFVGEAVPTAAPRNVVVHGATATQLDVTWEPPPLDSQNGDIQGYKIYFWEAQRGNLTERVKTLFL AENSVKLKNLTGYTAYMVSVAAFNAAGDGPRSTPTQGQTQQAAPSAPSSVKFSELTTTSVNVSWEAPQFP NGILEGYRLVYEPCSPVDGVSKIVTVDVKGNSPLWLKVKDLAEGVTYRFRIRAKTFTYGPEIEANVTTGP GEGAPGPPGVPIIVRYSSAIAIHWSSGDPGKGPITRYVIEARPSDEGLWDILIKDIPKEVSSYTFSMDIL KPGVSYDFRVIAVNDYGFGTPSSPSQSVPAQKANPFYEEWWFLVVIALVGLIFILLLVFVLIIRGQSKKY AKKTDSGNSAKSGALGHSEMMSLDESSFPALELNNRRLSVKNSFCRKNGLYTRSPPRPSPGSLHYSDEDV TKYNDLIPAESSSLTEKPSEISDSQGSDSEYEVDSNHQKAHSFVNHYISDPTYYNSWRRQQKGISRAQAY SYTESDSGEPDHTTVTNSTSTQQGSLFRPKASRTPTPQNPPNPPSQQSTLYRPPSSLAPGSRAPIAGFSS FV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001144952 |
| ORF Size | 6516 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001144952.2 |
| RefSeq Size | 10796 bp |
| RefSeq ORF | 6519 bp |
| Locus ID | 54549 |
| UniProt ID | Q58EX2 |
| Cytogenetics | 17q25.1 |
| Gene Summary | The protein encoded by this gene is a member of the immunoglobulin superfamily. The protein contains two immunoglobulin domains and thirteen fibronectin type III domains. Fibronectin type III domains are present in both extracellular and intracellular proteins and tandem repeats are known to contain binding sites for DNA, heparin and the cell surface. This protein, and a homologous mouse sequence, are very similar to the Drosophila sidekick gene product but the specific function of this superfamily member is not yet known. Evidence for alternative splicing at this gene locus has been observed but the full-length nature of additional variants has not yet been determined. [provided by RefSeq, Jul 2008] |
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