RNF156 (MGRN1) (NM_001142291) Human Tagged ORF Clone

CAT#: RG227477

MGRN1 (tGFP-tagged) - Human mahogunin, ring finger 1 (MGRN1), transcript variant 4

Product Images

Circular map for RG227477

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RNF156
• Synonyms: RNF156
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227477 representing NM_001142291
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCATTCTCAGCCGCCGCATCGCGGGGGTGGAGGACATCGACATCCAGGCGAACTCGGCCTATC
  GCTACCCTCCGAAGTCCGGAAACTACTTTGCTTCGCACTTTTTCATGGGAGGAGAGAAATTCGACACCCC
  CCACCCTGAAGGTTACCTCTTTGGAGAGAACATGGATCTGAACTTCCTGGGCAGCCGCCCGGTCCAGTTT
  CCCTACGTCACTCCTGCCCCCCACGAGCCCGTGAAGACGCTGCGGAGCCTGGTGAACATCCGCAAAGACT
  CCCTGCGGCTGGTGAGGTACAAAGACGATGCCGACAGCCCCACCGAGGACGGCGACAAGCCCCGGGTGCT
  CTACAGCCTGGAGTTCACCTTCGACGCCGATGCCCGCGTGGCCATCACCATCTACTGCCAGGCATCGGAG
  GAGTTCCTGAACGGCAGGGCAGTATACAGCCCCAAGAGCCCCTCGCTACAGTCCGAGACCGTCCACTACA
  AGAGAGGGGTGAGCCAGCAGTTCTCCCTGCCCTCCTTCAAGATTGACTTCTCGGAATGGAAGGATGACGA
  GCTGAACTTTGACCTGGACCGGGGCGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGAGATGCA
  GTGGTGGAAGTGACTGGCCACGCCCACGTGCTCTTGGCTGCCTTTGAAAAGCACATGGACGGCAGCTTCT
  CTGTGAAGCCTTTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTCCTGCAGGAGATCTATGGCAT
  TGAGAACAAGAACAACCAGGAGACCAAGCCCTCGGACGACGAGAACAGCGACAACAGCAACGAGTGTGTG
  GTGTGCCTGTCCGACCTGCGGGACACGCTGATCCTGCCCTGCCGCCACCTGTGCCTCTGTACCTCCTGCG
  CCGACACGCTGCGCTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCTTTCCGGGCCCTCCTGCAGAT
  CCGGGCGGTGCGGAAGAAGCCAGGAGCCCTGTCCCCCGTGTCCTTCAGCCCCGTCCTGGCCCAGAGCCTG
  GAGCATGATGAGCACTCTAACTCTGACAGCGTCCCACCTGGCTACGAGCCCATCTCGCTGCTCGAGGCGC
  TCAACGGCCTCCGGGCTGTCTCCCCGGCCATCCCCTCGGCCCCTCTTTATGAAGAAATCACCTATTCAGG
  CATCTCGGACGGCCTGTCCCAGGCCAGCTGTCCCCTCGCGGCTATCGACCACATCCTGGACAGCAGCCGC
  CAGAAGGGCAGGCCGCAGAGCAAGGCCCCCGACAGCACCCTACGGTCCCCGTCTTCCCCCATCCACGAAG
  AGGATGAGGAGAAGCTCTCCGAGGACGTGGACGCCCCTCCCCCACTGGGTGGCGCAGAGCTGGCCCTGCG
  GGAAAGCAGCTCCCCTGAGAGTTTCATAACAGAAGAGGTTGATGAGTCGTCGTCACCACAGCAAGGGACC
  CGAGCAGCTTCCATTGAGAATGTCCTGCAGGACAGCAGCCCCGAGCACTGTGGCCGAGGCCCACCTGCTG
  ACATCTACCTGCCAGCCCTGGGGCCCGACTCCTGCTCTGTTGGTATAGACGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227477 representing NM_001142291
  Red=Cloning site Green=Tags(s)
  
  MGSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEGYLFGENMDLNFLGSRPVQF
  PYVTPAPHEPVKTLRSLVNIRKDSLRLVRYKDDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASE
  EFLNGRAVYSPKSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVIQAVVDEGDA
  VVEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQEIYGIENKNNQETKPSDDENSDNSNECV
  VCLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSL
  EHDEHSNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSR
  QKGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITEEVDESSSPQQGT
  RAASIENVLQDSSPEHCGRGPPADIYLPALGPDSCSVGIDE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142291
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142291.1, NP_001135763.1
• RefSeq Size: 6407 bp
• RefSeq ORF: 1593 bp
• Locus ID: 23295
• UniProt ID: O60291
• Cytogenetics: 16p13.3
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.[supplied by OMIM, Apr 2004]