CNGB1 (NM_001135639) Human Tagged ORF Clone

CAT#: RG226950

CNGB1 (tGFP-tagged) - Human cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 2

Product Images

Circular map for RG226950

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CNGB1
• Synonyms: CNCG2; CNCG3L; CNCG4; CNG4; CNGB1B; GAR1; GARP; GARP2; RCNC2; RCNCb; RCNCbeta; RP45
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226950 representing NM_001135639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGGCTGGGTCCAGAGGGTGCTGCCTCAGCCCCCAGGGACCCCTCGGAAGACCAAGATGCAGGAGG
  AAGAGGAAGTGGAACCAGAGCCAGAGATGGAGGCGGAGGTGGAACCAGAACCGAATCCTGAGGAGGCCGA
  GACAGAGTCCGAGTCCATGCCCCCCGAAGAGTCATTCAAGGAGGAGGAAGTGGCTGTGGCAGACCCAAGC
  CCTCAGGAGACCAAGGAGGCTGCCCTTACTTCCACCATATCCCTCCGGGCCCAGGGCGCTGAGATTTCTG
  AAATGAATAGTCCCAGCCGCAGGGTACTGACCTGGCTCATGAAGGGCGTAGAGAAGGTGATCCCGCAGCC
  TGTTCACAGCATCACGGAGGACCCGGCTCAGATCCTGGGGCATGGCAGCACTGGGGACACAGGGTGCACA
  GATGAACCCAATGAGGCCCTTGAGGCCCAAGACACTAGGCCTGGGCTGCGGCTGCTTCTGTGGCTGGAGC
  AGAATCTGGAAAGAGTGCTTCCTCAGCCCCCCAAATCCTCTGAGGTCTGGAGAGATGAGCCTGCAGTTGC
  TACAGGTGCTGCCTCAGACCCAGCGCCTCCAGGACGCCCCCAGGAAATGGGGCCCAAGCTGCAGGCCCGG
  GAGACCCCCTCCCTGCCCACACCCATCCCCCTGCAGCCCAAGGAGGAACCCAAGGAGGCACCAGCTCCAG
  AGCCCCAGCCCGGCTCCCAGGCCCAGACCTCCTCCCTGCCACCAACCAGGGACCCTGCCAGGCTGGTGGC
  ATGGGTCCTGCACAGGCTGGAGATGGCCTTGCCGCAGCCAGTGCTACATGGGAAAATAGGGGAACAGGAG
  CCTGACTCCCCTGGGATATGTGATGTGCAGACCAGGGTGATGGGAGCTGGAGGTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226950 representing NM_001135639
  Red=Cloning site Green=Tags(s)
  
  MLGWVQRVLPQPPGTPRKTKMQEEEEVEPEPEMEAEVEPEPNPEEAETESESMPPEESFKEEEVAVADPS
  PQETKEAALTSTISLRAQGAEISEMNSPSRRVLTWLMKGVEKVIPQPVHSITEDPAQILGHGSTGDTGCT
  DEPNEALEAQDTRPGLRLLLWLEQNLERVLPQPPKSSEVWRDEPAVATGAASDPAPPGRPQEMGPKLQAR
  ETPSLPTPIPLQPKEEPKEAPAPEPQPGSQAQTSSLPPTRDPARLVAWVLHRLEMALPQPVLHGKIGEQE
  PDSPGICDVQTRVMGAGGL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135639
• ORF Size: 897 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135639.2
• RefSeq Size: 1648 bp
• RefSeq ORF: 900 bp
• Locus ID: 1258
• UniProt ID: Q14028
• Cytogenetics: 16q21
• Protein Families: Druggable Genome, Ion Channels: Cyclic nucleotide gated
• Protein Pathways: Olfactory transduction
• Gene Summary: In humans, the rod photoreceptor cGMP-gated cation channel helps regulate ion flow into the rod photoreceptor outer segment in response to light-induced alteration of the levels of intracellular cGMP. This channel consists of two subunits, alpha and beta, with the protein encoded by this gene representing the beta subunit. Defects in this gene are a cause of cause of retinitis pigmentosa type 45. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]