FES (NM_001143785) Human Tagged ORF Clone

CAT#: RG226923

FES (tGFP-tagged) - Human feline sarcoma oncogene (FES), transcript variant 4

Product Images

Circular map for RG226923

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FES
• Synonyms: FPS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226923 representing NM_001143785
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCTCTTCCGAGCTGTGCAGCCCCCAGGGCCACGGGGTCCTGCAGCAAATGCAGGAGGCCGAGC
  TTCGTCTACTGGAGGGCATGAGAAAGTGGATGGCCCAGCGGGTCAAGAGTGACAGGGAGTATGCAGGACT
  GCTTCACCACATGTCCCTGCAGGACAGTGGGGGCCAGAGCCGGGCCATCAGCCCTGACAGCCCCATCAGT
  CAGACCCACAGCCAGGACATTGAGAAGCTGAAGAGCCAGTACCGAGCTCTGGCACGGGACAGTGCCCAAG
  CCAAGCGCAAGTACCAGGAGGCCAGCAAAGACAAGGACCGTGACAAGGCTAAGGACAAGTATGTGCGCAG
  CCTGTGGAAGCTCTTTGCTCACCACAACCGCTATGTGCTGGGCGTGCGGGCTGCGCAGCTACACCACCAG
  CACCACCACCAGCTCCTGCTGCCCGGCCTGCTGCGGTCACTGCAGGACCTGCACGAGGAGATGGCTTGCA
  TCCTGAAGGAGATCCTGCAGGAATACCTGGAGATTAGCAGCCTGGTGCAGGATGAGGTGGTGGCCATTCA
  CCGGGAGATGGCTGCAGCTGCTGCCCGCATCCAGCCTGAGGCTGAGTACCAAGGCTTCCTGCGACAGTAT
  GGGTCCGCACCTGACGTCCCACCCTGTGTCACGTTCGATGAGTCACTGCTTGAGGAGGGTGAACCGCTGG
  AGCCTGGGGAGCTCCAGCTGAACGAGCTGACTGTGGAGAGCGTGCAGCACACGCTGACCTCAGTGACAGA
  TGAGCTGGCTGTGGCCACCGAGATGGTGTTCAGGCGGCAGGAGATGGTTACGCAGCTGCAACAGGAGCTC
  CGGAATGAAGAGGAGAACACCCACCCCCGGGAGCGGGTGCAGCTGCTGGGCAAGAGGCAAGTGCTGCAAG
  AAGCACTGCAGGGGCTGCAGGTAGCGCTGTGCAGCCAGGCCAAGCTGCAGGCCCAGCAGGAGTTGCTGCA
  GACCAAGCTGGAGCACCTGGGCCCCGGCGAGCCCCCGCCTGTGCTGCTCCTGCAGGATGACCGCCACTCC
  ACGTCGTCCTCGGAGCAGGAGCGAGAGGGGGGAAGGACACCCACGCTGGAGATCCTTAAGAGCCACATCT
  CAGGAATCTTCCGCCCCAAGTTCTCGAACCTGTACCGACTGGAAGGGGAAGGCTTTCCTAGCATTCCTTT
  GCTCATCGACCACCTACTGAGCACCCAGCAGCCCCTCACCAAGAAGAGTGGTGTTGTCCTGCACAGGGCT
  GTGCCCAAGGACAAGTGGGTGCTGAACCATGAGGACCTGGTGTTGGGTGAGCAGATTGGACGGGGGAACT
  TTGGCGAAGTGTTCAGCGGACGCCTGCGAGCCGACAACACCCTGGTGGCGGTGAAGTCTTGTCGAGAGAC
  GCTCCCACCTGACCTCAAGGCCAAGTTTCTACAGGAAGCGAGGATCCTGAAGCAGTACAGCCACCCCAAC
  ATCGTGCGTCTCATTGGTGTCTGCACCCAGAAGCAGCCCATCTACATCGTCATGGAGCTTGTGCAGGGGG
  GCGACTTCCTGACCTTCCTCCGCACGGAGGGGGCCCGCCTGCGGGTGAAGACTCTGCTGCAGATGGTGGG
  GGATGCAGCTGCTGGCATGGAGTACCTGGAGAGCAAGTGCTGCATCCACCGGGACCTGGCTGCTCGGAAC
  TGCCTGGTGACAGAGAAGAATGTCCTGAAGATCAGTGACTTTGGGATGTCCCGAGAGGAAGCCGATGGGG
  TCTATGCAGCCTCAGGGGGCCTCAGACAAGTCCCCGTGAAGTGGACCGCACCTGAGGCCCTTAACTACGG
  CCGCTACTCCTCCGAAAGCGACGTGTGGAGCTTTGGCATCTTGCTCTGGGAGACCTTCAGCCTGGGGGCC
  TCCCCCTATCCCAACCTCAGCAATCAGCAGACACGGGAGTTTGTGGAGAAGGGGGGCCGTCTGCCCTGCC
  CAGAGCTGTGTCCTGATGCCGTGTTCAGGCTCATGGAGCAGTGCTGGGCCTATGAGCCTGGGCAGCGGCC
  CAGCTTCAGCACCATCTACCAGGAGCTGCAGAGCATCCGAAAGCGGCATCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226923 representing NM_001143785
  Red=Cloning site Green=Tags(s)
  
  MGFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHMSLQDSGGQSRAISPDSPIS
  QTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQ
  HHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQY
  GSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQEL
  RNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPVLLLQDDRHS
  TSSSEQEREGGRTPTLEILKSHISGIFRPKFSNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHRA
  VPKDKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLPPDLKAKFLQEARILKQYSHPN
  IVRLIGVCTQKQPIYIVMELVQGGDFLTFLRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARN
  CLVTEKNVLKISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFGILLWETFSLGA
  SPYPNLSNQQTREFVEKGGRLPCPELCPDAVFRLMEQCWAYEPGQRPSFSTIYQELQSIRKRHR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143785
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143785.2
• RefSeq Size: 2422 bp
• RefSeq ORF: 2085 bp
• Locus ID: 2242
• UniProt ID: P07332
• Cytogenetics: 15q26.1
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.[provided by RefSeq, Jan 2009]