LSS (NM_001145436) Human Tagged ORF Clone

CAT#: RG226713

LSS (tGFP-tagged) - Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 3

Product Images

Circular map for RG226713

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LSS
• Synonyms: APMR4; CTRCT44; HYPT14; OSC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226713 representing NM_001145436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGAGGGCACGTGTCTGCGGCGCCGAGGGGGCCCCTACAAGACCGAGCCCGCCACCGACCTCGGCC
  GCTGGCGACTCAACTGCGAGAGGGGCCGGCAGACGTGGACCTACCTGCAGGACGAGCGCGCCGGCCGCGA
  GCAGACCGGCCTGGAAGCCTACGCCCTGGGGCTGGACACCAAGAATTACTTTAAGGACTTGCCCAAAGCC
  CACACCGCCTTTGAGGGGGCTCTGAACGGGATGACATTTTACGTGGGGCTGCAGGCTGAGGATGGGCACT
  GGACGGGTGATTATGGTGGCCCACTTTTCCTCCTGCCAGGCCTCCTGATCACTTGCCACGTGGCACGCAT
  CCCTCTGCCAGCCGGATACAGAGAAGAGATTGTGCGGTACCTGCGGCACATTGAGGATAAGTCCACCGTG
  TTTGGGACTGCGCTCAACTATGTGTCTCTCAGAATTCTGGGTGTTGGGCCTGACGATCCTGACCTGGTAC
  GAGCCCGGAACATTCTTCACAAGAAAGGTGGTGCTGTGGCCATCCCCTCCTGGGGGAAGTTCTGGCTGGC
  TGTCCTGAATGTTTACAGCTGGGAAGGCCTCAATACCCTGTTCCCAGAGATGTGGCTGTTTCCTGACTGG
  GCACCGGCACACCCCTCCACACTCTGGTGCCACTGCCGGCAGGTGTACCTGCCCATGAGCTACTGCTACG
  CCGTTCGGCTGAGTGCCGCGGAAGACCCGCTGGTCCAGAGCCTCCGCCAGGAGCTCTATGTGGAGGACTT
  CGCCAGCATTGACTGGCTGGCGCAGAGGAACAACGTGGCCCCCGACGAGCTGTACACGCCGCACAGCTGG
  CTGCTCCGCGTGGTATATGCGCTCCTCAACCTGTATGAGCACCACCACAGTGCCCACCTGCGGCAGCGGG
  CCGTGCAGAAGCTGTATGAACACATTGTGGCCGACGACCGATTCACCAAGAGCATCAGCATCGGCCCGAT
  CTCGAAAACCATCAACATGCTTGTGCGCTGGTATGTGGACGGGCCCGCCTCCACTGCCTTCCAGGAGCAT
  GTCTCCAGAATCCCGGACTATCTCTGGATGGGCCTTGACGGCATGAAAATGCAGGGCACCAACGGCTCAC
  AGATCTGGGACACCGCATTCGCCATCCAGGCTCTGCTTGAGGCGGGCGGGCACCACAGGCCCGAGTTTTC
  GTCCTGCCTGCAGAAGGCTCATGAGTTCCTGAGGCTCTCACAGGTCCCAGATAACCCTCCCGACTACCAG
  AAGTACTACCGCCAGATGCGCAAGGGTGGCTTCTCCTTCAGTACGCTGGACTGCGGCTGGATCGTTTCTG
  ACTGCACGGCTGAGGCCTTGAAGGCTGTGCTGCTCCTGCAGGAGAAGTGTCCCCATGTCACCGAGCACAT
  CCCCAGAGAACGGCTCTGCGATGCTGTGGCTGTGCTGCTGAACATGAGAAATCCAGATGGAGGGTTCGCC
  ACCTATGAGACCAAGCGTGGGGGGCACTTGCTGGAGCTGCTGAACCCCTCGGAGGTCTTCGGGGACATCA
  TGATTGACTACACCTATGTGGAGTGCACCTCAGCCGTGATGCAGGCGCTTAAGTATTTCCACAAGCGTTT
  CCCGGAGCACAGGGCAGCGGAGATCCGGGAGACCCTCACGCAGGGCTTAGAGTTCTGTCGGCGGCAGCAG
  AGGGCCGATGGCTCCTGGGAAGGCTCCTGGGGAGTTTGCTTCACCTACGGCACCTGGTTTGGCCTGGAGG
  CCTTCGCCTGTATGGGGCAGACCTACCGAGATGGGACTGCCTGTGCAGAGGTCTCCCGGGCCTGTGACTT
  CCTGCTGTCCCGGCAGATGGCAGACGGAGGCTGGGGGGAGGACTTTGAGTCCTGCGAGGAGCGGCGTTAT
  TTGCAGAGTGCCCAGTCCCAGATCCATAACACATGCTGGGCCATGATGGGGCTGATGGCCGTTCGGCATC
  CTGACATCGAGGCCCAGGAGAGAGGAGTCCGGTGTCTACTTGAGAAACAGCTCCCCAATGGCGACTGGCC
  GCAGGAAAACATTGCTGGGGTCTTCAACAAGTCCTGTGCCATCTCCTACACGAGCTACAGGAACATCTTC
  CCCATCTGGGCCCTCGGCCGCTTCTCCCAGCTGTACCCTGAGAGAGCCCTTGCTGGCCACCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226713 representing NM_001145436
  Red=Cloning site Green=Tags(s)
  
  MTEGTCLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYALGLDTKNYFKDLPKA
  HTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLLPGLLITCHVARIPLPAGYREEIVRYLRHIEDKSTV
  FGTALNYVSLRILGVGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGLNTLFPEMWLFPDW
  APAHPSTLWCHCRQVYLPMSYCYAVRLSAAEDPLVQSLRQELYVEDFASIDWLAQRNNVAPDELYTPHSW
  LLRVVYALLNLYEHHHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYVDGPASTAFQEH
  VSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRLSQVPDNPPDYQ
  KYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFA
  TYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETLTQGLEFCRRQQ
  RADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWGEDFESCEERRY
  LQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIF
  PIWALGRFSQLYPERALAGHP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145436
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145436.2
• RefSeq Size: 4225 bp
• RefSeq ORF: 2166 bp
• Locus ID: 4047
• UniProt ID: P48449
• Cytogenetics: 21q22.3
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: The protein encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2009]