CASK (NM_001126054) Human Tagged ORF Clone

CAT#: RG226244

CASK (tGFP-tagged) - Human calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 2

Product Images

Circular map for RG226244

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CASK
• Synonyms: CAGH39; CAMGUK; CMG; FGS4; hCASK; LIN2; MICPCH; MRXSNA; TNRC8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226244 representing NM_001126054
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACGACGACGTGCTGTTCGAGGATGTGTACGAGCTGTGCGAGGTGATCGGAAAGGGTCCCTTCA
  GTGTTGTACGACGATGTATCAACAGAGAAACTGGGCAACAATTTGCTGTAAAAATTGTTGATGTAGCCAA
  GTTCACATCAAGTCCAGGGTTAAGTACAGAAGATCTAAAGCGGGAAGCCAGTATCTGTCATATGCTGAAA
  CATCCACACATTGTAGAGTTATTGGAGACATATAGCTCAGATGGAATGCTTTACATGGTTTTCGAATTTA
  TGGATGGAGCAGATCTGTGTTTTGAAATCGTAAAGCGAGCTGACGCTGGTTTTGTGTACAGTGAAGCTGT
  AGCCAGCCATTATATGAGACAGATACTGGAAGCTCTACGCTACTGCCATGATAATAACATAATTCACAGG
  GATGTGAAGCCCCACTGTGTTCTCCTTGCCTCAAAAGAAAACTCGGCACCTGTTAAACTTGGAGGCTTTG
  GGGTAGCTATTCAATTAGGGGAGTCTGGACTTGTAGCTGGAGGACGTGTTGGAACACCTCATTTTATGGC
  ACCAGAAGTGGTCAAAAGAGAGCCTTACGGAAAGCCTGTAGACGTCTGGGGGTGCGGTGTGATCCTTTTT
  ATCCTGCTCAGTGGTTGTTTGCCTTTTTACGGAACCAAGGAAAGATTGTTTGAAGGCATTATTAAAGGAA
  AATATAAGATGAATCCAAGGCAGTGGAGCCATATCTCTGAAAGTGCCAAAGACCTAGTACGTCGCATGCT
  GATGCTGGATCCAGCTGAAAGGATCACTGTTTATGAAGCACTGAATCACCCATGGCTTAAGGAGCGGGAT
  CGTTACGCCTACAAGATTCATCTTCCAGAAACAGTAGAGCAGCTGAGGAAATTCAATGCAAGGAGGAAAC
  TAAAGGGTGCAGTACTAGCCGCTGTGTCAAGTCACAAATTCAACTCATTCTATGGGGATCCCCCTGAAGA
  GTTACCAGATTTCTCCGAAGACCCTACCTCCTCAGGACTTCTAGCAGCAGAAAGAGCAGTCTCACAGGTG
  CTGGACAGCCTGGAAGAGATTCATGCGCTTACAGACTGCAGTGAAAAGGACCTAGATTTTCTACACAGTG
  TTTTCCAGGATCAGCATCTTCACACACTACTAGATCTGTATGACAAAATTAACACAAAGTCTTCACCACA
  AATCAGGAATCCTCCAAGCGATGCAGTACAGAGAGCCAAAGAGGTATTGGAAGAAATTTCATGTTACCCT
  GAGAATAACGACGCAAAGGAACTAAAGCGTATTTTAACACAACCTCATTTCATGGCCTTACTTCAGACTC
  ACGACGTAGTGGCACATGAAGTTTACAGTGATGAAGCATTGAGGGTCACACCTCCTCCCACCTCTCCCTA
  TTTAAACGGCGATTCTCCAGAAAGTGCTAACGGAGACATGGATATGGAGAATGTGACCAGAGTTCGGCTG
  GTACAGTTTCAAAAGAACACAGATGAACCAATGGGAATCACTTTAAAAATGAATGAACTAAATCATTGTA
  TTGTTGCAAGAATTATGCATGGGGGCATGATTCACAGGCAAGGTACACTTCATGTTGGTGATGAAATTCG
  AGAAATCAATGGCATCAGTGTGGCTAACCAAACAGTGGAACAACTGCAAAAAATGCTTAGGGAAATGCGG
  GGGAGTATTACCTTCAAGATTGTGCCAAGTTACCGCACTCAGTCTTCGTCCTGTGAGGACTTGCCATCAA
  CTACCCAACCAAAAGGACGACAGATCTATGTAAGAGCACAATTTGAATATGATCCAGCCAAGGATGACCT
  CATCCCCTGTAAAGAAGCTGGCATTCGATTCAGAGTTGGTGACATCATCCAGATTATTAGTAAGGATGAT
  CATAATTGGTGGCAGGGTAAACTGGAAAACTCCAAAAATGGAACTGCAGGTCTCATTCCTTCTCCTGAAC
  TTCAGGAATGGCGAGTAGCTTGCATTGCCATGGAGAAGACCAAACAGGAGCAGCAGGCCAGCTGTACTTG
  GTTTGGCAAGAAAAAGAAGCAGTACAAAGATAAATATTTGGCAAAGCACAATGCAGATCTTGTCACATAT
  GAAGAAGTAGTAAAACTGCCAGCATTCAAGAGGAAAACACTAGTCTTATTAGGCGCACATGGTGTTGGGA
  GAAGACACATAAAAAACACTCTCATCACAAAGCACCCAGACCGGTTTGCGTACCCTATTCCACATACAAC
  CAGACCTCCAAAGAAAGACGAAGAAAATGGAAAGAATTATTACTTTGTATCTCATGACCAAATGATGCAA
  GACATCTCTAATAACGAGTACTTGGAGTACGGCAGCCACGAGGATGCGATGTATGGGACAAAACTGGAGA
  CCATCCGGAAGATCCACGAGCAGGGGCTGATTGCAATACTGGACGTGGAGCCTCAGGCACTGAAGGTCCT
  GAGAACTGCAGAGTTTGCTCCTTTTGTTGTTTTCATTGCTGCACCAACTATTACTCCAGGTTTAAATGAG
  GATGAATCTCTTCAGCGTCTGCAGAAGGAGTCTGACATCTTACAGAGAACATATGCACACTACTTCGATC
  TCACAATTATCAACAATGAAATTGATGAGACAATCAGACATCTGGAGGAAGCTGTTGAGCTCGTGTGCAC
  AGCCCCACAGTGGGTCCCTGTCTCCTGGGTCTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226244 representing NM_001126054
  Red=Cloning site Green=Tags(s)
  
  MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLK
  HPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHR
  DVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILF
  ILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERD
  RYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGLLAAERAVSQV
  LDSLEEIHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYP
  ENNDAKELKRILTQPHFMALLQTHDVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRL
  VQFQKNTDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMR
  GSITFKIVPSYRTQSSSCEDLPSTTQPKGRQIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDD
  HNWWQGKLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNADLVTY
  EEVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPDRFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQ
  DISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNE
  DESLQRLQKESDILQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001126054
• ORF Size: 2694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001126054.2, NP_001119526.1
• RefSeq Size: 8229 bp
• RefSeq ORF: 2697 bp
• Locus ID: 8573
• UniProt ID: O14936
• Cytogenetics: Xp11.4
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Tight junction
• Gene Summary: This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, intellectual disability and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked intellectual disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]