ZNF83 (NM_001105552) Human Tagged ORF Clone

CAT#: RG225888

ZNF83 (tGFP-tagged) - Human zinc finger protein 83 (ZNF83), transcript variant 4

Product Images

Circular map for RG225888

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF83
• Synonyms: HPF1; ZNF816B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225888 representing NM_001105552
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGGGAGAAAGGATGATGCACAAAAGCAGCCTGTTAAAAATCAGCTTGGATTAAACCCGCAGTCAC
  ATCTACCAGAACTGCAGCTATTTCAAGCTGAAGGGAAAATATATAAATATGATCACATGGAAAAATCTGT
  CAACAGTAGTTCCTTAGTTTCCCCACCCCAACGTATTTCTTCTACTGTCAAAACCCACATTTCTCATACA
  TATGAATGTAATTTTGTGGATTCATTATTCACACAAAAAGAGAAAGCAAATATTGGGACAGAACACTACA
  AATGTAGTGAGCGTGGCAAGGCCTTTCATCAAGGCTTACATTTTACTATACATCAAATAATCCATACTAA
  AGAGACGCAATTTAAATGTGATATATGTGGCAAGATCTTCAATAAAAAATCAAACCTTGCAAGTCATCAA
  AGAATTCATACTGGAGAGAAGCCATATAAATGTAATGAATGTGGCAAGGTCTTCCATAATATGTCACACC
  TTGCACAGCATCGCAGGATTCATACTGGAGAGAAACCATATAAATGTAATGAATGTGGCAAGGTCTTTAA
  TCAAATTTCACACCTTGCACAACATCAAAGAATTCATACCGGAGAGAAACCTTATAAATGTAATGAATGT
  GGAAAGGTCTTCCATCAAATTTCACACCTTGCACAACATCGGACAATTCATACTGGAGAAAAACCTTACG
  AATGTAACAAATGTGGCAAGGTGTTCAGTCGCAATTCCTACCTTGTACAACATCTGATCATTCATACTGG
  AGAGAAACCTTACAGATGTAATGTATGTGGAAAGGTCTTCCATCATATTTCACACCTTGCACAACATCAG
  AGAATCCACACTGGAGAGAAACCTTACAAATGTAATGAGTGTGGCAAGGTCTTCAGTCACAAGTCATCCC
  TAGTAAATCACTGGAGAATTCATACTGGAGAGAAACCTTACAAATGTAATGAGTGTGGCAAGGTCTTCAG
  TCACAAGTCATCCCTAGTAAATCACTGGAGAATCCACACTGGAGAGAAACCTTACAAATGTAATGAATGT
  GGCAAGGTCTTCAGTCGCAATTCATACCTTGCCCAACATCTGATAATTCATGCCGGTGAGAAACCTTATA
  AGTGTGATGAATGTGACAAAGCATTCAGTCAAAATTCACATCTTGTACAACATCACAGAATCCATACTGG
  AGAGAAACCTTACAAATGTGATGAATGTGGCAAAGTCTTCAGTCAAAATTCATACCTTGCATATCATTGG
  AGAATTCATACTGGAGAAAAAGCTTATAAATGTAATGAATGTGGGAAGGTCTTCGGTCTAAACTCATCCC
  TAGCACATCATCGGAAAATTCACACTGGAGAGAAACCTTTCAAATGTAATGAATGTGGCAAAGCTTTTAG
  TATGCGTTCAAGCCTCACTAATCATCATGCGATCCACACTGGAGAGAAACATTTCAAATGTAATGAATGT
  GGCAAACTCTTCCGCGACAATTCATATCTTGTACGTCATCAGAGATTTCATGCCGGAAAGAAATCTAACA
  CATGTAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225888 representing NM_001105552
  Red=Cloning site Green=Tags(s)
  
  MHGRKDDAQKQPVKNQLGLNPQSHLPELQLFQAEGKIYKYDHMEKSVNSSSLVSPPQRISSTVKTHISHT
  YECNFVDSLFTQKEKANIGTEHYKCSERGKAFHQGLHFTIHQIIHTKETQFKCDICGKIFNKKSNLASHQ
  RIHTGEKPYKCNECGKVFHNMSHLAQHRRIHTGEKPYKCNECGKVFNQISHLAQHQRIHTGEKPYKCNEC
  GKVFHQISHLAQHRTIHTGEKPYECNKCGKVFSRNSYLVQHLIIHTGEKPYRCNVCGKVFHHISHLAQHQ
  RIHTGEKPYKCNECGKVFSHKSSLVNHWRIHTGEKPYKCNECGKVFSHKSSLVNHWRIHTGEKPYKCNEC
  GKVFSRNSYLAQHLIIHAGEKPYKCDECDKAFSQNSHLVQHHRIHTGEKPYKCDECGKVFSQNSYLAYHW
  RIHTGEKAYKCNECGKVFGLNSSLAHHRKIHTGEKPFKCNECGKAFSMRSSLTNHHAIHTGEKHFKCNEC
  GKLFRDNSYLVRHQRFHAGKKSNTCN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105552
• ORF Size: 1548 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105552.1, NP_001099022.1
• RefSeq Size: 2749 bp
• RefSeq ORF: 1551 bp
• Locus ID: 55769
• UniProt ID: P51522
• Cytogenetics: 19q13.41
• Protein Families: Transcription Factors
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]