Upstream Binding Protein 1 (UBP1) (NM_001128160) Human Tagged ORF Clone

CAT#: RG225866

UBP1 (tGFP-tagged) - Human upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 2

Product Images

Circular map for RG225866

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Upstream Binding Protein 1
• Synonyms: LBP-1a; LBP-1B; LBP1A; LBP1B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225866 representing NM_001128160
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGGTGCTCAAGATGGACGAGGTGATCGAGTCCGGGCTGGTGCACGACTTCGACGCCAGCCTCT
  CGGGCATCGGGCAGGAACTGGGCGCCGGCGCTTACAGCATGAGTGATGTCTTGGCATTGCCCATTTTCAA
  GCAGGAAGATTCCAGCCTTCCATTGGATGGTGAAACAGAGCACCCACCCTTTCAGTATGTGATGTGTGCT
  GCAACGTCACCAGCAGTAAAACTGCATGATGAAACGCTTACTTATTTGAACCAAGGTCAGTCATATGAAA
  TTCGGATGCTGGATAATCGGAAAATGGGTGATATGCCTGAGATCAATGGAAAATTAGTAAAGAGCATCAT
  AAGGGTTGTATTCCATGACAGACGGCTACAATACACAGAGCATCAGCAACTTGAAGGATGGAAGTGGAAT
  CGCCCAGGAGACAGACTTCTTGATTTAGATATTCCAATGTCTGTGGGAATAATTGACACAAGGACGAATC
  CAAGCCAGTTAAATGCGGTTGAATTTCTGTGGGACCCAGCAAAACGCACCTCTGCTTTCATTCAGGTACA
  CTGCATCAGCACAGAATTTACTCCACGGAAGCACGGAGGTGAAAAGGGAGTGCCCTTTAGGATCCAGGTT
  GACACCTTTAAGCAGAATGAAAATGGAGAATACACAGATCATCTACACTCAGCTAGCTGCCAAATCAAAG
  TTTTTAAGCCTAAAGGTGCAGACAGGAAACAAAAAACTGACCGAGAGAAGATGGAGAAGAGAACAGCTCA
  TGAAAAAGAAAAGTATCAGCCGTCCTATGATACCACAATCCTCACAGAGTGTTCTCCGTGGCCCGATGCC
  CCCACAGCCTATGTGAATAACAGCCCTTCCCCAGCGCCCACTTTCACCTCCCCACAGCAGAGCACTTGCA
  GTGTCCCAGACAGCAATTCTTCTTCCCCAAATCATCAGGGAGATGGAGCTTCACAGACCTCTGGTGAACA
  AATTCAGCCTTCAGCTACGATCCAGGAAACACAGCAATGGCTGCTCAAAAACAGATTCTCTTCCTACACA
  AGACTGTTCTCTAATTTTTCAGGTGCCGACTTATTAAAACTGACAAAGGAGGATTTAGTTCAAATTTGTG
  GTGCAGCCGATGGAATTCGGCTCTATAATTCACTGAAGTCAAGGTCGGTTAGACCCCGTTTAACCATCTA
  TGTCTGCCGGGAGCAGCCAAGCAGCACAGTGCTGCAAGGGCAGCAGCAAGCTGCAAGCAGTGCAAGCGAG
  AATGGCAGTGGGGCACCCTATGTTTATCATGCAATCTACTTGGAAGAAATGATTGCCTCAGAAGTTGCTC
  GAAAACTTGCGCTGGTGTTTAATATCCCTCTCCACCAAATTAATCAGGTTTACAGACAGGGTCCCACCGG
  TATTCACATTCTTGTTAGTGATCAGATGGTTCAGAATTTTCAAGATGAGAGTTGTTTTTTATTCTCCACA
  GTAAAAGCTGAAAGTAGTGATGGCATCCACATAATTTTGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225866 representing NM_001128160
  Red=Cloning site Green=Tags(s)
  
  MAWVLKMDEVIESGLVHDFDASLSGIGQELGAGAYSMSDVLALPIFKQEDSSLPLDGETEHPPFQYVMCA
  ATSPAVKLHDETLTYLNQGQSYEIRMLDNRKMGDMPEINGKLVKSIIRVVFHDRRLQYTEHQQLEGWKWN
  RPGDRLLDLDIPMSVGIIDTRTNPSQLNAVEFLWDPAKRTSAFIQVHCISTEFTPRKHGGEKGVPFRIQV
  DTFKQNENGEYTDHLHSASCQIKVFKPKGADRKQKTDREKMEKRTAHEKEKYQPSYDTTILTECSPWPDA
  PTAYVNNSPSPAPTFTSPQQSTCSVPDSNSSSPNHQGDGASQTSGEQIQPSATIQETQQWLLKNRFSSYT
  RLFSNFSGADLLKLTKEDLVQICGAADGIRLYNSLKSRSVRPRLTIYVCREQPSSTVLQGQQQAASSASE
  NGSGAPYVYHAIYLEEMIASEVARKLALVFNIPLHQINQVYRQGPTGIHILVSDQMVQNFQDESCFLFST
  VKAESSDGIHIILK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128160
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128160.1, NP_001121632.1
• RefSeq Size: 4054 bp
• RefSeq ORF: 1515 bp
• Locus ID: 7342
• UniProt ID: Q9NZI7
• Cytogenetics: 3p22.3
• Protein Families: Transcription Factors
• Gene Summary: Functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.[UniProtKB/Swiss-Prot Function]