Pannexin 3 (PANX3) (NM_052959) Human Tagged ORF Clone

CAT#: RG224924

PANX3 (tGFP-tagged) - Human pannexin 3 (PANX3)

Product Images

Circular map for RG224924

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pannexin 3
• Synonyms: PX3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224924 representing NM_052959
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACTTGCACACACAGCTGCAGAGTACATGCTCTCAGATGCCCTGCTGCCTGACCGCAGGGGACCCC
  GCCTCAAAGGACTGCGTCTGGAACTGCCCCTGGACCGGATAGTCAAGTTCGTAGCTGTGGGCTCCCCCTT
  GTTGCTGATGTCCCTGGCATTCGCCCAGGAGTTCTCCTCTGGGTCTCCGATCAGCTGCTTCTCTCCCAGT
  AACTTCAGCATCCGGCAGGCAGCCTACGTGGACAGCTCCTGCTGGGACTCACTGCTTCACCATAAGCAGG
  ACGGGCCTGGCCAGGACAAAATGAAATCTCTCTGGCCCCACAAGGCCCTCCCCTACTCCCTGCTGGCCCT
  GGCCTTGCTCATGTACCTGCCGGTGCTGCTGTGGCAGTATGCAGCTGTGCCAGCCCTCAGCTCCGATCTG
  CTGTTCATCATCAGCGAACTGGACAAATCTTATAATCGCTCCATCCGCCTCGTGCAGCACATGCTGAAGA
  TCCGGCAGAAGAGTTCCGACCCCTATGTGTTCTGGAATGAGCTGGAGAAGGCTCGGAAAGAACGATACTT
  TGAATTCCCTTTGCTAGAGCGGTACCTGGCATGTAAGCAGCGTTCACATTCGCTAGTGGCTACCTACCTC
  CTGAGGAACTCCCTCTTGCTCATCTTCACCTCCGCCACTTACCTATACCTTGGTCATTTCCATCTGGATG
  TCTTCTTCCAGGAAGAATTCAGCTGCTCCATCAAGACAGGGCTGCTAAGTGATGAGACCCATGTCCCCAA
  TCTGATCACATGCAGGCTGACATCACTGTCCATTTTCCAGATTGTTAGCCTCTCCAGTGTAGCAATATAC
  ACCATATTGGTTCCAGTGATAATATACAACCTCACACGGCTATGTCGGTGGGACAAACGACTTTTATCTG
  TCTATGAGATGCTCCCAGCTTTTGATCTCCTCAGCAGAAAGATGCTAGGATGTCCCATCAATGACCTCAA
  TGTGATCCTTCTTTTCCTCCGAGCTAACATCTCTGAGCTCATCTCTTTTAGCTGGCTGAGTGTCTTATGT
  GTGTTGAAGGATACAACCACCCAGAAGCACAATATTGACACAGTAGTTGATTTTATGACTTTATTGGCTG
  GCTTAGAACCCTCAAAACCCAAACACCTCACCAACTCGGCATGTGATGAACACCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224924 representing NM_052959
  Red=Cloning site Green=Tags(s)
  
  MSLAHTAAEYMLSDALLPDRRGPRLKGLRLELPLDRIVKFVAVGSPLLLMSLAFAQEFSSGSPISCFSPS
  NFSIRQAAYVDSSCWDSLLHHKQDGPGQDKMKSLWPHKALPYSLLALALLMYLPVLLWQYAAVPALSSDL
  LFIISELDKSYNRSIRLVQHMLKIRQKSSDPYVFWNELEKARKERYFEFPLLERYLACKQRSHSLVATYL
  LRNSLLLIFTSATYLYLGHFHLDVFFQEEFSCSIKTGLLSDETHVPNLITCRLTSLSIFQIVSLSSVAIY
  TILVPVIIYNLTRLCRWDKRLLSVYEMLPAFDLLSRKMLGCPINDLNVILLFLRANISELISFSWLSVLC
  VLKDTTTQKHNIDTVVDFMTLLAGLEPSKPKHLTNSACDEHP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052959
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052959.3
• RefSeq Size: 1609 bp
• RefSeq ORF: 1179 bp
• Locus ID: 116337
• UniProt ID: Q96QZ0
• Cytogenetics: 11q24.2
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene belongs to the innexin family. Innexin family members are known to be the structural components of gap junctions. [provided by RefSeq, Jul 2008]