Phospholipase C beta 3 (PLCB3) (NM_000932) Human Tagged ORF Clone

CAT#: RG224268

PLCB3 (tGFP-tagged) - Human phospholipase C, beta 3 (phosphatidylinositol-specific) (PLCB3), transcript variant 1

Product Images

Circular map for RG224268

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Phospholipase C beta 3
• Synonyms: SMDCD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224268 representing NM_000932
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCCCAGCCCGGCGTCCACGCGCTGCAGTTGGAGCCGCCCACCGTGGTGGAGACCCTGCGGC
  GCGGGAGTAAGTTCATCAAATGGGACGAGGAGACCTCCAGTCGGAACCTGGTGACCCTGCGTGTGGACCC
  CAATGGCTTCTTCTTGTACTGGACGGGCCCCAACATGGAGGTGGACACACTGGACATCAGTTCCATCAGG
  GACACACGGACAGGCCGGTACGCCCGCCTGCCCAAGGACCCCAAGATCCGGGAAGTTCTGGGCTTTGGGG
  GTCCCGATGCCCGGCTGGAGGAGAAGCTGATGACGGTGGTGTCTGGGCCAGACCCGGTGAACACAGTGTT
  CTTGAACTTCATGGCCGTGCAGGATGACACAGCCAAGGTCTGGTCTGAGGAGCTATTCAAGCTGGCTATG
  AACATCCTGGCTCAGAACGCCTCCCGGAACACCTTCCTGCGCAAAGCATACACGAAGCTGAAGCTGCAGG
  TGAACCAGGATGGTCGGATCCCCGTCAAGAACATCCTGAAGATGTTCTCAGCAGACAAGAAGCGGGTGGA
  GACTGCGCTGGAATCCTGTGGCCTCAAATTCAACCGGAGTGAGTCCATCCGGCCTGATGAGTTTTCCTTG
  GAAATCTTTGAGCGGTTCCTGAACAAGCTGTGTCTGCGGCCGGACATTGACAAGATCCTGCTGGAGATAG
  GCGCCAAGGGCAAGCCATACCTGACGCTGGAGCAGCTCATGGACTTCATCAACCAGAAGCAACGCGACCC
  GAGACTCAACGAAGTGCTGTACCCGCCCCTGCGGCCCTCCCAGGCCCGGCTGCTCATCGAAAAGTATGAG
  CCCAACCAGCAGTTTCTGGAGCGAGACCAGATGTCCATGGAGGGCTTTAGCCGCTACCTGGGAGGCGAGG
  AGAATGGCATCCTGCCCCTGGAAGCCCTGGATCTGAGCACGGACATGACCCAGCCACTGAGTGCCTACTT
  CATCAACTCCTCGCATAACACCTATCTCACTGCGGGGCAGCTGGCTGGGACCTCGTCGGTGGAGATGTAC
  CGCCAGGCACTACTATGGGGCTGCCGCTGCGTGGAGCTGGACGTGTGGAAGGGACGGCCGCCTGAGGAGG
  AACCCTTCATTACCCACGGCTTCACCATGACCACAGAGGTGCCTCTGCGCGACGTGCTGGAGGCCATTGC
  CGAGACTGCCTTCAAGACCTCGCCCTACCCCGTCATCCTCTCCTTCGAGAACCATGTGGACTCGGCAAAG
  CAACAGGCAAAGATGGCTGAGTACTGCCGCTCCATCTTTGGAGACGCGCTACTCATCGAGCCTCTGGACA
  AGTACCCGCTGGCCCCAGGCGTTCCCCTGCCCAGCCCCCAGGACCTGATGGGCCGTATCCTGGTGAAGAA
  CAAGAAGCGGCACCGACCCAGCGCAGGTGGCCCAGACAGCGCCGGGCGCAAGCGGCCCCTGGAGCAGAGC
  AATTCTGCCCTGAGCGAGAGCTCCGCGGCCACCGAGCCCTCCTCCCCGCAGCTGGGGTCTCCCAGCTCTG
  ACAGCTGCCCAGGCCTGAGCAATGGGGAGGAGGTAGGGCTTGAGAAGCCCAGCCTGGAGCCTCAGAAGTC
  TCTGGGTGACGAGGGCCTGAACCGAGGCCCCTATGTTCTTGGACCTGCTGACCGTGAGGATGAGGAGGAA
  GATGAGGAAGAGGAGGAACAGACAGACCCCAAAAAGCCAACTACAGATGAGGGCACAGCCAGCAGCGAGG
  TGAATGCCACTGAGGAGATGTCCACGCTTGTCAACTACATCGAACCTGTCAAGTTCAAGTCCTTTGAGGC
  TGCTCGAAAGAGGAACAAATGCTTCGAGATGTCGTCCTTTGTGGAGACCAAGGCCATGGAGCAACTGACC
  AAGAGCCCCATGGAGTTTGTGGAATACAACAAGCAGCAGCTCAGCCGCATCTACCCCAAGGGCACCCGCG
  TGGACTCCTCCAACTACATGCCCCAGCTCTTCTGGAACGTAGGGTGCCAGCTTGTTGCGCTCAACTTCCA
  GACCCTCGATGTGGCGATGCAGCTCAACGCGGGCGTTTTTGAGTACAACGGGCGCAGCGGGTACCTGCTC
  AAGCCGGAGTTCATGCGGCGGCCGGACAAGTCCTTCGACCCCTTCACTGAGGTCATCGTGGATGGCATCG
  TGGCCAATGCCTTGCGGGTCAAGGTGATCTCAGGGCAGTTCCTGTCCGACAGGAAGGTGGGCATCTACGT
  GGAGGTGGACATGTTTGGCCTCCCTGTTGATACGCGGCGCAAGTACCGCACCCGGACCTCTCAGGGGAAC
  TCGTTCAACCCCGTGTGGGACGAAGAGCCCTTCGACTTCCCCAAGGTGGTGCTGCCCACGCTGGCTTCAC
  TTCGCATTGCAGCCTTTGAGGAGGGGGGTAAATTCGTAGGGCACCGGATCCTGCCTGTCTCTGCCATCCG
  CTCCGGATACCACTACGTCTGCCTGCGGAACGAGGCCAACCAACCGCTGTGCCTGCCGGCCCTGCTCATC
  TACACCGAAGCCTCGGACTACATTCCTGACGACCACCAGGACTATGCGGAGGCCCTGATCAACCCCATTA
  AGCACGTCAGCCTGATGGACCAGAGGGCCCGGCAGCTGGCCGCCCTCATTGGGGAGAGTGAGGCTCAGGC
  TGGCCAAGAGACGTGCCAGGACACCCAGTCTCAGCAGCTGGGGTCTCAGCCGTCCTCAAACCCCACCCCC
  AGCCCACTGGATGCCTCCCCCCGCCGGCCCCCTGGCCCCACCACCTCCCCTGCCAGCACCTCCCTCAGCA
  GCCCAGGGCAGCGTGATGATCTCATCGCCAGCATCCTCTCAGAGGTGGCCCCCACCCCGCTGGATGAGCT
  CCGAGGTCACAAGGCTCTGGTCAAGCTCCGGAGCCGGCAAGAGCGAGACCTGCGGGAGCTGCGCAAGAAG
  CATCAGCGGAAGGCAGTCACCCTCACCCGCCGCCTGCTGGATGGCCTGGCTCAGGCACAGGCTGAGGGCA
  GGTGCCGGCTGCGGCCAGGTGCCCTAGGTGGGGCCGCTGATGTGGAGGACACGAAGGAGGGGGAGGACGA
  GGCAAAGCGGTATCAGGAGTTCCAGAACAGACAGGTGCAGAGCCTGCTGGAGCTGCGGGAGGCCCAGGTG
  GACGCAGAGGCCCAGCGGAGGCTGGAACACCTGAGACAGGCTCTGCAGCGGCTCAGGGAGGTCGTCCTTG
  ATGCAAACACAACTCAGTTCAAGAGGCTGAAAGAGATGAACGAGAGGGAGAAGAAGGAGCTGCAGAAGAT
  CCTGGACAGAAAGCGCCATAACAGCATCTCGGAGGCCAAGATGAGGGACAAGCATAAGAAGGAGGCGGAA
  CTGACGGAGATTAACCGTCGGCACATCACTGAGTCAGTCAACTCCATCCGTCGGCTGGAGGAGGCCCAGA
  AGCAGCGGCATGACCGTCTTGTGGCTGGGCAGCAGCAGGTCCTGCAACAGCTGGCAGAAGAGGAGCCCAA
  GCTGCTGGCCCAGCTGGCCCAGGAGTGTCAGGAGCAGCGGGCGAGGCTCCCCCAGGAGATCCGCCGGAGC
  CTGCTGGGCGAGATGCCGGAGGGGCTGGGGGACGGGCCTCTGGTGGCCTGTGCCAGCAACGGTCACGCAC
  CCGGGAGCAGCGGGCACCTGTCGGGCGCTGACTCGGAGAGCCAGGAGGAGAACACGCAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224268 representing NM_000932
  Red=Cloning site Green=Tags(s)
  
  MAGAQPGVHALQLEPPTVVETLRRGSKFIKWDEETSSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIR
  DTRTGRYARLPKDPKIREVLGFGGPDARLEEKLMTVVSGPDPVNTVFLNFMAVQDDTAKVWSEELFKLAM
  NILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLKFNRSESIRPDEFSL
  EIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRPSQARLLIEKYE
  PNQQFLERDQMSMEGFSRYLGGEENGILPLEALDLSTDMTQPLSAYFINSSHNTYLTAGQLAGTSSVEMY
  RQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAETAFKTSPYPVILSFENHVDSAK
  QQAKMAEYCRSIFGDALLIEPLDKYPLAPGVPLPSPQDLMGRILVKNKKRHRPSAGGPDSAGRKRPLEQS
  NSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKPSLEPQKSLGDEGLNRGPYVLGPADREDEEE
  DEEEEEQTDPKKPTTDEGTASSEVNATEEMSTLVNYIEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLT
  KSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDVAMQLNAGVFEYNGRSGYLL
  KPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDRKVGIYVEVDMFGLPVDTRRKYRTRTSQGN
  SFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLI
  YTEASDYIPDDHQDYAEALINPIKHVSLMDQRARQLAALIGESEAQAGQETCQDTQSQQLGSQPSSNPTP
  SPLDASPRRPPGPTTSPASTSLSSPGQRDDLIASILSEVAPTPLDELRGHKALVKLRSRQERDLRELRKK
  HQRKAVTLTRRLLDGLAQAQAEGRCRLRPGALGGAADVEDTKEGEDEAKRYQEFQNRQVQSLLELREAQV
  DAEAQRRLEHLRQALQRLREVVLDANTTQFKRLKEMNEREKKELQKILDRKRHNSISEAKMRDKHKKEAE
  LTEINRRHITESVNSIRRLEEAQKQRHDRLVAGQQQVLQQLAEEEPKLLAQLAQECQEQRARLPQEIRRS
  LLGEMPEGLGDGPLVACASNGHAPGSSGHLSGADSESQEENTQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000932
• ORF Size: 3702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000932.1, NP_000923.1
• RefSeq Size: 3705 bp
• RefSeq ORF: 3705 bp
• Locus ID: 5331
• UniProt ID: Q01970
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Vascular smooth muscle contraction, Wnt signaling pathway
• Gene Summary: This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]