ABCB11 (NM_003742) Human Tagged ORF Clone

CAT#: RG224122

ABCB11 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 11 (ABCB11)

Product Images

Circular map for RG224122

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ABCB11
• Synonyms: ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4; SPGP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224122 representing NM_003742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACTCAGTAATTCTTCGAAGTATAAAGAAATTTGGAGAGGAGAATGATGGTTTTGAGTCAGATA
  AATCATATAATAATGATAAGAAATCAAGGTTACAAGATGAGAAGAAAGGTGATGGCGTTAGAGTTGGCTT
  CTTTCAATTGTTTCGGTTTTCTTCATCAACTGACATTTGGCTGATGTTTGTGGGAAGTTTGTGTGCATTT
  CTCCATGGAATAGCCCAGCCAGGCGTGCTACTCATTTTTGGCACAATGACAGATGTTTTTATTGACTACG
  ACGTTGAGTTACAAGAACTCCAGATTCCAGGAAAAGCATGTGTGAATAACACCATTGTATGGACTAACAG
  TTCCCTCAACCAGAACATGACAAATGGAACACGTTGTGGGTTGCTGAACATCGAGAGCGAAATGATCAAA
  TTTGCCAGTTACTATGCTGGAATTGCTGTCGCAGTACTTATCACAGGATATATTCAAATATGCTTTTGGG
  TCATTGCCGCAGCTCGTCAGATACAGAAAATGAGAAAATTTTACTTTAGGAGAATAATGAGAATGGAAAT
  AGGGTGGTTTGACTGCAATTCAGTGGGGGAGCTGAATACAAGATTCTCTGATGATATTAATAAAATCAAT
  GATGCCATAGCTGACCAAATGGCCCTTTTCATTCAGCGCATGACCTCGACCATCTGTGGTTTCCTGTTGG
  GATTTTTCAGGGGTTGGAAACTGACCTTGGTTATTATTTCTGTCAGCCCTCTCATTGGGATTGGAGCAGC
  CACCATTGGTCTGAGTGTGTCCAAGTTTACGGACTATGAGCTGAAGGCCTATGCCAAAGCAGGGGTGGTG
  GCTGATGAAGTCATTTCATCAATGAGAACAGTGGCTGCTTTTGGTGGTGAGAAAAGAGAGGTTGAAAGGT
  ATGAGAAAAATCTTGTGTTCGCCCAGCGTTGGGGAATTAGAAAAGGAATAGTGATGGGATTCTTTACTGG
  ATTCGTGTGGTGTCTCATCTTTTTGTGTTATGCACTGGCCTTCTGGTACGGCTCCACACTTGTCCTGGAT
  GAAGGAGAATATACACCAGGAACCCTTGTCCAGATTTTCCTCAGTGTCATAGTAGGAGCTTTAAATCTTG
  GCAATGCCTCTCCTTGTTTGGAAGCCTTTGCAACTGGACGTGCAGCAGCCACCAGCATTTTTGAGACAAT
  AGACAGGAAACCCATCATTGACTGCATGTCAGAAGATGGTTACAAGTTGGATCGAATCAAGGGTGAAATT
  GAATTCCATAATGTGACCTTCCATTATCCTTCCAGACCAGAGGTGAAGATTCTAAATGACCTCAACATGG
  TCATTAAACCAGGGGAAATGACAGCTCTGGTAGGACCCAGTGGAGCTGGAAAAAGTACAGCACTGCAACT
  CATTCAGCGATTCTATGACCCCTGTGAAGGAATGGTGACCGTGGATGGCCATGACATTCGCTCTCTTAAC
  ATTCAGTGGCTTAGAGATCAGATTGGGATAGTGGAGCAAGAGCCAGTTCTGTTCTCTACCACCATTGCAG
  AAAATATTCGCTATGGCAGAGAAGATGCAACAATGGAAGACATAGTCCAAGCTGCCAAGGAGGCCAATGC
  CTACAACTTCATCATGGACCTGCCACAGCAATTTGACACCCTTGTTGGAGAAGGAGGAGGCCAGATGAGT
  GGTGGCCAGAAACAAAGGGTAGCTATCGCCAGAGCCCTCATCCGAAATCCCAAGATTCTGCTTTTGGACA
  TGGCCACCTCAGCTCTGGACAATGAGAGTGAAGCCATGGTGCAAGAAGTGCTGAGTAAGATTCAGCATGG
  GCACACAATCATTTCAGTTGCTCATCGCTTGTCTACGGTCAGAGCTGCAGATACCATCATTGGTTTTGAA
  CATGGCACTGCAGTGGAAAGAGGGACCCATGAAGAATTACTGGAAAGGAAAGGTGTTTACTTCACTCTAG
  TGACTTTGCAAAGCCAGGGAAATCAAGCTCTTAATGAAGAGGACATAAAGGATGCAACTGAAGATGACAT
  GCTTGCGAGGACCTTTAGCAGAGGGAGCTACCAGGATAGTTTAAGGGCTTCCATCCGGCAACGCTCCAAG
  TCTCAGCTTTCTTACCTGGTGCACGAACCTCCATTAGCTGTTGTAGATCATAAGTCTACCTATGAAGAAG
  ATAGAAAGGACAAGGACATTCCTGTGCAGGAAGAAGTTGAACCTGCCCCAGTTAGGAGGATTCTGAAATT
  CAGTGCTCCAGAATGGCCCTACATGCTGGTAGGGTCTGTGGGTGCAGCTGTGAACGGGACAGTCACACCC
  TTGTATGCCTTTTTATTCAGCCAGATTCTTGGGACTTTTTCAATTCCTGATAAAGAGGAACAAAGGTCAC
  AGATCAATGGTGTGTGCCTACTTTTTGTAGCAATGGGCTGTGTATCTCTTTTCACCCAATTTCTACAGGG
  ATATGCCTTTGCTAAATCTGGGGAGCTCCTAACAAAAAGGCTACGTAAATTTGGTTTCAGGGCAATGCTG
  GGGCAAGATATTGCCTGGTTTGATGACCTCAGAAATAGCCCTGGAGCATTGACAACAAGACTTGCTACAG
  ATGCTTCCCAAGTTCAAGGGGCTGCCGGCTCTCAGATCGGGATGATAGTCAATTCCTTCACTAACGTCAC
  TGTGGCCATGATCATTGCCTTCTCCTTTAGCTGGAAGCTGAGCCTGGTCATCTTGTGCTTCTTCCCCTTC
  TTGGCTTTATCAGGAGCCACACAGACCAGGATGTTGACAGGATTTGCCTCTCGAGATAAGCAGGCCCTGG
  AGATGGTGGGACAGATTACAAATGAAGCCCTCAGTAACATCCGCACTGTTGCTGGAATTGGAAAGGAGAG
  GCGGTTCATTGAAGCACTTGAGACTGAGCTGGAGAAGCCCTTCAAGACAGCCATTCAGAAAGCCAATATT
  TACGGATTCTGCTTTGCCTTTGCCCAGTGCATCATGTTTATTGCGAATTCTGCTTCCTACAGATATGGAG
  GTTACTTAATCTCCAATGAGGGGCTCCATTTCAGCTATGTGTTCAGGGTGATCTCTGCAGTTGTACTGAG
  TGCAACAGCTCTTGGAAGAGCCTTCTCTTACACCCCAAGTTATGCAAAAGCTAAAATATCAGCTGCACGC
  TTTTTTCAACTGCTGGACCGACAACCCCCAATCAGTGTATACAATACTGCAGGTGAAAAATGGGACAACT
  TCCAGGGGAAGATTGATTTTGTTGATTGTAAATTTACATATCCTTCTCGACCTGACTCGCAAGTTCTGAA
  TGGTCTCTCAGTGTCGATTAGTCCAGGGCAGACACTGGCGTTTGTTGGGAGCAGTGGATGTGGCAAAAGC
  ACTAGCATTCAGCTGTTGGAACGTTTCTATGATCCTGATCAAGGGAAGGTGATGATAGATGGTCATGACA
  GCAAAAAAGTAAATGTCCAGTTCCTCCGCTCAAACATTGGAATTGTTTCCCAGGAACCAGTGTTGTTTGC
  CTGTAGCATAATGGACAATATCAAGTATGGAGACAACACCAAAGAAATTCCCATGGAAAGAGTCATAGCA
  GCTGCAAAACAGGCTCAGCTGCATGATTTTGTCATGTCACTCCCAGAGAAATATGAAACTAACGTTGGGT
  CCCAGGGGTCTCAACTCTCTAGAGGGGAGAAACAACGCATTGCTATTGCTCGGGCCATTGTACGAGATCC
  TAAAATCTTGCTACTAGATGAAGCCACTTCTGCCTTAGACACAGAAAGTGAAAAGACGGTGCAGGTTGCT
  CTAGACAAAGCCAGAGAGGGTCGGACCTGCATTGTCATTGCCCATCGCTTGTCCACCATCCAGAACGCGG
  ATATCATTGCTGTCATGGCACAGGGGGTGGTGATTGAAAAGGGGACCCATGAAGAACTGATGGCCCAAAA
  AGGAGCCTACTACAAACTAGTCACCACTGGATCCCCCATCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224122 representing NM_003742
  Red=Cloning site Green=Tags(s)
  
  MSDSVILRSIKKFGEENDGFESDKSYNNDKKSRLQDEKKGDGVRVGFFQLFRFSSSTDIWLMFVGSLCAF
  LHGIAQPGVLLIFGTMTDVFIDYDVELQELQIPGKACVNNTIVWTNSSLNQNMTNGTRCGLLNIESEMIK
  FASYYAGIAVAVLITGYIQICFWVIAAARQIQKMRKFYFRRIMRMEIGWFDCNSVGELNTRFSDDINKIN
  DAIADQMALFIQRMTSTICGFLLGFFRGWKLTLVIISVSPLIGIGAATIGLSVSKFTDYELKAYAKAGVV
  ADEVISSMRTVAAFGGEKREVERYEKNLVFAQRWGIRKGIVMGFFTGFVWCLIFLCYALAFWYGSTLVLD
  EGEYTPGTLVQIFLSVIVGALNLGNASPCLEAFATGRAAATSIFETIDRKPIIDCMSEDGYKLDRIKGEI
  EFHNVTFHYPSRPEVKILNDLNMVIKPGEMTALVGPSGAGKSTALQLIQRFYDPCEGMVTVDGHDIRSLN
  IQWLRDQIGIVEQEPVLFSTTIAENIRYGREDATMEDIVQAAKEANAYNFIMDLPQQFDTLVGEGGGQMS
  GGQKQRVAIARALIRNPKILLLDMATSALDNESEAMVQEVLSKIQHGHTIISVAHRLSTVRAADTIIGFE
  HGTAVERGTHEELLERKGVYFTLVTLQSQGNQALNEEDIKDATEDDMLARTFSRGSYQDSLRASIRQRSK
  SQLSYLVHEPPLAVVDHKSTYEEDRKDKDIPVQEEVEPAPVRRILKFSAPEWPYMLVGSVGAAVNGTVTP
  LYAFLFSQILGTFSIPDKEEQRSQINGVCLLFVAMGCVSLFTQFLQGYAFAKSGELLTKRLRKFGFRAML
  GQDIAWFDDLRNSPGALTTRLATDASQVQGAAGSQIGMIVNSFTNVTVAMIIAFSFSWKLSLVILCFFPF
  LALSGATQTRMLTGFASRDKQALEMVGQITNEALSNIRTVAGIGKERRFIEALETELEKPFKTAIQKANI
  YGFCFAFAQCIMFIANSASYRYGGYLISNEGLHFSYVFRVISAVVLSATALGRAFSYTPSYAKAKISAAR
  FFQLLDRQPPISVYNTAGEKWDNFQGKIDFVDCKFTYPSRPDSQVLNGLSVSISPGQTLAFVGSSGCGKS
  TSIQLLERFYDPDQGKVMIDGHDSKKVNVQFLRSNIGIVSQEPVLFACSIMDNIKYGDNTKEIPMERVIA
  AAKQAQLHDFVMSLPEKYETNVGSQGSQLSRGEKQRIAIARAIVRDPKILLLDEATSALDTESEKTVQVA
  LDKAREGRTCIVIAHRLSTIQNADIIAVMAQGVVIEKGTHEELMAQKGAYYKLVTTGSPIS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003742
• ORF Size: 3963 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003742.4
• RefSeq Size: 4775 bp
• RefSeq ORF: 3966 bp
• Locus ID: 8647
• UniProt ID: O95342
• Cytogenetics: 2q31.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]