EDG7 (LPAR3) (NM_012152) Human Tagged ORF Clone

CAT#: RG223706

LPAR3 (tGFP-tagged) - Human lysophosphatidic acid receptor 3 (LPAR3)

Product Images

Circular map for RG223706

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EDG7
• Synonyms: Edg-7; EDG7; GPCR; HOFNH30; LP-A3; LPA3; RP4-678I3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223706 representing NM_012152
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAGTGTCACTATGACAAGCACATGGACTTTTTTTATAATAGGAGCAACACTGATACTGTCGATG
  ACTGGACAGGAACAAAGCTTGTGATTGTTTTGTGTGTTGGGACGTTTTTCTGCCTGTTTATTTTTTTTTC
  TAATTCTCTGGTCATCGCGGCAGTGATCAAAAACAGAAAATTTCATTTCCCCTTCTACTACCTGTTGGCT
  AATTTAGCTGCTGCCGATTTCTTCGCTGGAATTGCCTATGTATTCCTGATGTTTAACACAGGCCCAGTTT
  CAAAAACTTTGACTGTCAACCGCTGGTTTCTCCGTCAGGGGCTTCTGGACAGTAGCTTGACTGCTTCCCT
  CACCAACTTGCTGGTTATCGCCGTGGAGAGGCACATGTCAATCATGAGGATGCGGGTCCATAGCAACCTG
  ACCAAAAAGAGGGTGACACTGCTCATTTTGCTTGTCTGGGCCATCGCCATTTTTATGGGGGCGGTCCCCA
  CACTGGGCTGGAATTGCCTCTGCAACATCTCTGCCTGCTCTTCCCTGGCCCCCATTTACAGCAGGAGTTA
  CCTTGTTTTCTGGACAGTGTCCAACCTCATGGCCTTCCTCATCATGGTTGTGGTGTACCTGCGGATCTAC
  GTGTACGTCAAGAGGAAAACCAACGTCTTGTCTCCGCATACAAGTGGGTCCATCAGCCGCCGGAGGACAC
  CCATGAAGCTAATGAAGACGGTGATGACTGTCTTAGGGGCGTTTGTGGTATGCTGGACCCCGGGCCTGGT
  GGTTCTGCTCCTCGACGGCCTGAACTGCAGGCAGTGTGGCGTGCAGCATGTGAAAAGGTGGTTCCTGCTG
  CTGGCGCTGCTCAACTCCGTCGTGAACCCCATCATCTACTCCTACAAGGACGAGGACATGTATGGCACCA
  TGAAGAAGATGATCTGCTGCTTCTCTCAGGAGAACCCAGAGAGGCGTCCCTCTCGCATCCCCTCCACAGT
  CCTCAGCAGGAGTGACACAGGCAGCCAGTACATAGAGGATAGTATTAGCCAAGGTGCAGTCTGCAATAAA
  AGCACTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223706 representing NM_012152
  Red=Cloning site Green=Tags(s)
  
  MNECHYDKHMDFFYNRSNTDTVDDWTGTKLVIVLCVGTFFCLFIFFSNSLVIAAVIKNRKFHFPFYYLLA
  NLAAADFFAGIAYVFLMFNTGPVSKTLTVNRWFLRQGLLDSSLTASLTNLLVIAVERHMSIMRMRVHSNL
  TKKRVTLLILLVWAIAIFMGAVPTLGWNCLCNISACSSLAPIYSRSYLVFWTVSNLMAFLIMVVVYLRIY
  VYVKRKTNVLSPHTSGSISRRRTPMKLMKTVMTVLGAFVVCWTPGLVVLLLDGLNCRQCGVQHVKRWFLL
  LALLNSVVNPIIYSYKDEDMYGTMKKMICCFSQENPERRPSRIPSTVLSRSDTGSQYIEDSISQGAVCNK
  STS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012152
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012152.3
• RefSeq Size: 1148 bp
• RefSeq ORF: 1062 bp
• Locus ID: 23566
• UniProt ID: Q9UBY5
• Cytogenetics: 1p22.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of the G protein-coupled receptor family, as well as the EDG family of proteins. This protein functions as a cellular receptor for lysophosphatidic acid and mediates lysophosphatidic acid-evoked calcium mobilization. This receptor couples predominantly to G(q/11) alpha proteins. [provided by RefSeq, Jul 2008]