ZNF419A (ZNF419) (NM_001098496) Human Tagged ORF Clone

CAT#: RG223388

ZNF419 (tGFP-tagged) - Human zinc finger protein 419 (ZNF419), transcript variant 7. Note: ORF is codon optimized

Product Images

Circular map for RG223388

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF419A
• Synonyms: ZAPHIR; ZNF419A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223388 ORF sequence, codon optimized.
  Due to the complexity of NM_001098496, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGCGGCTTTGCGGGACCCAGCCCAGGGATACGTGACATTCGAGGACGTGGCAGTTTACTTCT
  CTCAAGAAGAGTGGCGGCTGCTCGACGATGCACAGAGACTGCTTTATCGCAACGTTATGCTCGAAAACTT
  CACCCTGTTGGCCAGTCTGGGCTGCTGGCATGGGGCTGAGGCCGAAGAAGCCCCAGAGCAGATCGCTTCT
  GTGGGTCTGTTGTCAAGTAATATCCAGCAGCACCAGAAGCAGCATTGCGGAGAAAAACCCCTGAAAAGAC
  AGGAAGGGCGCGTGCCCGTGCTTCGATCATGCAAAGTACATCTCAGTGAGAAGTCTCTGCAAAGTAGAGA
  AGTAGGCAAAGCCCTGCTGATCTCTTCAGGTGTGTTGAAGCACCAAGTGACTCATACAGGCGAGAAGTCT
  CATCGCAGCAGTAAGAGCAGGGAAGCTTTCCACGCCGGGAAGCGCCACTACAAGTGTAGCGAGTGCGGGA
  AAGCATTCGGTCAGAAGTATCTTTTGGTGCAGCACCAGCGGCTGCATGCCGGCAAAAAAACGTACGAATG
  CTCAGAATGCGGGAAACTGTTTAGGGATATGAGCAACCTTTTCATACATCAAATCGTCCATACCGGAGAA
  AGACCTTACGGATGTTCAAATTGCGGTAAGTCCTTCAGCAGGAACGCACACCTGATTGAACACCAGCGCG
  TGCACACGGGAGAAAAACCCTTTACCTGTTCCGAGTGCGGCAAGGCGTTTAGACATAATTCTACGCTGGT
  ACAGCATCATAAAATCCACACTGGGGTTAGGCCTTATGAGTGTTCAGAATGTGGCAAACTGTTCAGTTTC
  AACTCTAGTCTGATGAAACACCAACGCATACACACCGGCGAGCGACCATATAAATGCTCCGAGTGTGGCA
  AGTTTTACTCTCACAAGAGCAATCTGATCAAACATTGGAGAGTCCACACCGGAGAGCGACCCTATAAGTG
  TAGCGACTGTGGCAAATTCTTCACGCAGTGCTCCTCACTGATGCAGCATCAGAAAGTCCACACCGGTGAG
  AAGCCCTTCAAATGTAATGAATGCGGCAGATTCTTTCGGGAGAATTCAACTTTGGTTCGCCACCAGCGAG
  TGCATACGGGCGCAAAGCCGTATGAGTGCAGAGAGTGCGGGAAATTCTTTTCTCAATCCTCCACTCTCAT
  GCAGCACCGGAAAGTGCACATCGGCGAAAAGCCCTTCAAGTGCAACGAGTGTGGGAGGCTCTTTCGCGAA
  AACAGCTCATTGGTGAAGCATCAGCGGGTCCACACCGGGGCTAAGCCCTACGAATGCAGGGAGTGCGGCA
  AATTCTTCCGACACAACTCCAGTCTCTTCAAGCATCGGCGGATTCATACAGGAGAAATGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223388 representing NM_001098496
  Red=Cloning site Green=Tags(s)
  
  MAAAALRDPAQGYVTFEDVAVYFSQEEWRLLDDAQRLLYRNVMLENFTLLASLGCWHGAEAEEAPEQIAS
  VGLLSSNIQQHQKQHCGEKPLKRQEGRVPVLRSCKVHLSEKSLQSREVGKALLISSGVLKHQVTHTGEKS
  HRSSKSREAFHAGKRHYKCSECGKAFGQKYLLVQHQRLHAGKKTYECSECGKLFRDMSNLFIHQIVHTGE
  RPYGCSNCGKSFSRNAHLIEHQRVHTGEKPFTCSECGKAFRHNSTLVQHHKIHTGVRPYECSECGKLFSF
  NSSLMKHQRIHTGERPYKCSECGKFYSHKSNLIKHWRVHTGERPYKCSDCGKFFTQCSSLMQHQKVHTGE
  KPFKCNECGRFFRENSTLVRHQRVHTGAKPYECRECGKFFSQSSTLMQHRKVHIGEKPFKCNECGRLFRE
  NSSLVKHQRVHTGAKPYECRECGKFFRHNSSLFKHRRIHTGEMQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098496
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098496.1, NP_001091966.1
• RefSeq Size: 2243 bp
• RefSeq ORF: 1395 bp
• Locus ID: 79744
• UniProt ID: Q96HQ0
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]