PRPF38B (NM_018061) Human Tagged ORF Clone

CAT#: RG223346

PRPF38B (tGFP-tagged) - Human PRP38 pre-mRNA processing factor 38 (yeast) domain containing B (PRPF38B), transcript variant 1

Product Images

Circular map for RG223346

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRPF38B
• Synonyms: NET1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223346 representing NM_018061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAACAACAGCCCCGCGCTGACAGGCAACTCGCAGCCGCAGCACCAGGCGGCTGCAGCTGCGGCTC
  AGCAACAGCAGCAGTGCGGCGGCGGCGGCGCTACCAAGCCGGCGGTCTCCGGCAAGCAGGGCAATGTGCT
  CCCGCTCTGGGGCAGCGAGAAGACCATGAACCTCAACCCCATGATCCTGACCAACATCCTGTCGTCGCCT
  TACTTCAAAGTACAGCTCTACGAGCTCAAGACCTACCACGAGGTGGTGGACGAGATCTACTTTAAGGTCA
  CGCACGTTGAACCATGGGAGAAAGGAAGCAGGAAAACAGCGGGCCAGACAGGGATGTGCGGAGGGGTTCG
  AGGTGTTGGAACAGGAGGAATTGTTTCTACAGCATTTTGCCTGTTATACAAATTATTTACCCTGAAGTTA
  ACTCGAAAGCAAGTGATGGGTCTTATAACACACACAGACTCTCCATATATTAGAGCGCTTGGATTTATGT
  ATATAAGATATACACAGCCCCCTACAGATCTGTGGGACTGGTTTGAATCCTTCCTTGATGATGAAGAGGA
  CCTAGATGTGAAGGCTGGTGGAGGCTGTGTAATGACCATTGGAGAAATGCTACGATCTTTTCTCACAAAA
  CTGGAGTGGTTTTCTACCTTGTTTCCAAGAATTCCAGTTCCAGTTCAAAAGAATATTGATCAACAGATTA
  AAACCCGACCTAGAAAAATCAAGAAAGATGGGAAGGAAGGTGCTGAGGAAATAGACAGACATGTTGAACG
  CAGACGTTCAAGGTCTCCAAGGAGATCTCTGAGTCCACGGAGGTCCCCAAGAAGGTCAAGAAGTAGAAGT
  CATCATCGGGAGGGCCATGGGTCTTCTAGTTTTGACAGAGAATTAGAAAGAGAGAAAGAACGCCAGCGAC
  TAGAGCGTGAAGCCAAAGAAAGGGAGAAAGAACGGCGAAGATCCCGAAGTATTGACCGGGGGTTAGAACG
  CAGGCGCAGCAGAAGTAGGGAAAGGCATAGAAGTCGCAGTCGAAGTCGTGATAGGAAAGGGGATAGAAGG
  GACAGGGATCGAGAAAGAGAGAAAGAAAATGAGAGAGGTAGAAGACGAGATCGTGACTATGATAAGGAAA
  GAGGAAATGAACGAGAAAAAGAGAGAGAGCGATCAAGAGAAAGGTCCAAGGAACAGAGAAGTAGGGGAGA
  GGTAGAAGAGAAGAAACATAAAGAAGACAAAGATGATAGGCGGCACAGAGATGACAAAAGAGATTCCAAG
  AAAGAGAAAAAACACAGTAGAAGCAGAAGCAGAGAAAGGAAACACAGAAGTAGGAGTCGAAGTAGAAATG
  CAGGGAAACGAAGTAGAAGTAGAAGCAAAGAGAAATCAAGTAAACATAAAAATGAAAGTAAAGAAAAATC
  AAATAAACGAAGTCGAAGTGGCAGTCAAGGAAGAACTGACAGTGTTGAAAAATCAAAAAAACGGGAACAT
  AGTCCCAGCAAAGAAAAATCTAGAAAGCGTAGTAGAAGCAAAGAACGTTCCCACAAACGAGATCACAGTG
  ATAGTAAGGACCAGTCAGACAAACATGATCGTCGAAGGAGCCAAAGTATAGAACAAGAGAGCCAAGAAAA
  ACAGCATAAAAACAAAGATGAGACTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223346 representing NM_018061
  Red=Cloning site Green=Tags(s)
  
  MANNSPALTGNSQPQHQAAAAAAQQQQQCGGGGATKPAVSGKQGNVLPLWGSEKTMNLNPMILTNILSSP
  YFKVQLYELKTYHEVVDEIYFKVTHVEPWEKGSRKTAGQTGMCGGVRGVGTGGIVSTAFCLLYKLFTLKL
  TRKQVMGLITHTDSPYIRALGFMYIRYTQPPTDLWDWFESFLDDEEDLDVKAGGGCVMTIGEMLRSFLTK
  LEWFSTLFPRIPVPVQKNIDQQIKTRPRKIKKDGKEGAEEIDRHVERRRSRSPRRSLSPRRSPRRSRSRS
  HHREGHGSSSFDRELEREKERQRLEREAKEREKERRRSRSIDRGLERRRSRSRERHRSRSRSRDRKGDRR
  DRDREREKENERGRRRDRDYDKERGNEREKERERSRERSKEQRSRGEVEEKKHKEDKDDRRHRDDKRDSK
  KEKKHSRSRSRERKHRSRSRSRNAGKRSRSRSKEKSSKHKNESKEKSNKRSRSGSQGRTDSVEKSKKREH
  SPSKEKSRKRSRSKERSHKRDHSDSKDQSDKHDRRRSQSIEQESQEKQHKNKDETV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018061
• ORF Size: 1638 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018061.1, NP_060531.1
• RefSeq Size: 3239 bp
• RefSeq ORF: 1641 bp
• Locus ID: 55119
• UniProt ID: Q5VTL8
• Cytogenetics: 1p13.3
• Domains: PRP38
• Protein Pathways: Spliceosome
• Gene Summary: May be required for pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]