NABC1 (BCAS1) (NM_003657) Human Tagged ORF Clone

CAT#: RG223329

BCAS1 (tGFP-tagged) - Human breast carcinoma amplified sequence 1 (BCAS1)

Product Images

Circular map for RG223329

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NABC1
• Synonyms: AIBC1; NABC1; PMES-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223329 representing NM_003657
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTAACCAAATGAGTGTTCCCCAAAGAGTTGAAGACCAAGAGAATGAACCAGAAGCAGAGACTTACC
  AGGACAACGCGTCTGCTCTGAACGGGGTTCCAGTGGTGGTGTCGACCCACACAGTTCAGCACTTAGAGGA
  AGTCGACTTGGGAATAAGTGTCAAGACGGATAATGTGGCCACTTCTTCCCCCGAGACAACGGAGATAAGT
  GCTGTTGCGGATGCCAACGGAAAGAATCTTGGGAAAGAGGCCAAACCCGAGGCACCAGCTGCTAAATCTC
  GTTTTTTCTTGATGCTCTCTCGGCCTGTACCAGGACGTACCGGAGACCAAGCCGCAGATTCATCCCTTGG
  ATCAGTGAAGCTTGATGTCAGCTCCAATAAAGCTCCAGCGAACAAAGACCCAAGTGAGAGCTGGACACTT
  CCGGTGGCAGCTGGACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCC
  TCTCTGCCGCCAGGGATCCCACGCTTCTCCCACCTGAGACAGGGGGAGCAGGAGGAGAAGCTCCCTCCAA
  GCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGCCAGGTGAC
  AGCCAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGT
  CCGATGATGTCCCTGCAGGGAAGGACATAGTTGACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGC
  GGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGATTCCCAGGCAGCAGCTATA
  GCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAA
  AGGACCCAGAAGACACGGGTGCTGAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAA
  CTTTACATCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACCCATCGGGGCACACACAG
  TCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCAAGGAGAAATCAGGACCCACCTCTCTGCCTCTGG
  GCAAACTGTTTTGGAAAAAGTCAGTTAAAGAGGACTCAGTCCCCACAGGTGCGGAGGAGAATGTGGTGTG
  TGAGTCACCAGTAGAGATTATAAAGTCCAAGGAAGTAGAATCAGCCTTACAAACAGTGGACCTCAACGAA
  GGAGATGCTGCACCTGAACCCACAGAAGCGAAACTCAAAAGAGAAGAAAGCAAACCAAGAACCTCTCTGA
  TGGCGTTTCTCAGACAAATGTCAGTGAAAGGGGATGGAGGGATCACCCACTCAGAAGAAATAAATGGGAA
  AGACTCCAGCTGCCAAACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACCAACAGGA
  GCACCACAGAAGGGTAAAGAGGGCTCCTCGAAGGACAAGAAGTCAGCAGCCGAGATGAACAAGCAGAAGA
  GCAACAAGCAGGAAGCCAAAGAACCAGCCCAGTGCACAGAGCAGGCCACGGTGGACACGAACTCACTGCA
  GAATGGGGACAAGCTCCAAAAGAGACCTGAGAAGCGGCAGCAGTCCCTTGGGGGCTTCTTTAAAGGCCTG
  GGACCAAAGCGGATGTTGGATGCTCAAGTGCAAACAGACCCAGTATCCATCGGACCAGTTGGCAAACCCA
  AG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223329 representing NM_003657
  Red=Cloning site Green=Tags(s)
  
  MGNQMSVPQRVEDQENEPEAETYQDNASALNGVPVVVSTHTVQHLEEVDLGISVKTDNVATSSPETTEIS
  AVADANGKNLGKEAKPEAPAAKSRFFLMLSRPVPGRTGDQAADSSLGSVKLDVSSNKAPANKDPSESWTL
  PVAAGPGQDTDKTPGHAPAQDKVLSAARDPTLLPPETGGAGGEAPSKPKDSSFFDKFFKLDKGQEKVPGD
  SQQEAKRAEHQDKVDEVPGLSGQSDDVPAGKDIVDGKEKEGQELGTADCSVPGDPEGLETAKDDSQAAAI
  AENNNSIMSFFKTLVSPNKAETKKDPEDTGAEKSPTTSADLKSDKANFTSQETQGAGKNSKGCNPSGHTQ
  SVTTPEPAKEGTKEKSGPTSLPLGKLFWKKSVKEDSVPTGAEENVVCESPVEIIKSKEVESALQTVDLNE
  GDAAPEPTEAKLKREESKPRTSLMAFLRQMSVKGDGGITHSEEINGKDSSCQTSDSTEKTITPPEPEPTG
  APQKGKEGSSKDKKSAAEMNKQKSNKQEAKEPAQCTEQATVDTNSLQNGDKLQKRPEKRQQSLGGFFKGL
  GPKRMLDAQVQTDPVSIGPVGKPK
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003657
• ORF Size: 1752 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003657.1, NP_003648.1
• RefSeq Size: 2813 bp
• RefSeq ORF: 1755 bp
• Locus ID: 8537
• UniProt ID: O75363
• Cytogenetics: 20q13.2
• Gene Summary: This gene resides in a region at 20q13 which is amplified in a variety of tumor types and associated with more aggressive tumor phenotypes. Among the genes identified from this region, it was found to be highly expressed in three amplified breast cancer cell lines and in one breast tumor without amplification at 20q13.2. However, this gene is not in the common region of maximal amplification and its expression was not detected in the breast cancer cell line MCF7, in which this region is highly amplified. Although not consistently expressed, this gene is a candidate oncogene. [provided by RefSeq, Apr 2016]