TNPO3 (NM_012470) Human Tagged ORF Clone

CAT#: RG223103

TNPO3 (tGFP-tagged) - Human transportin 3 (TNPO3), transcript variant 1. Note: ORF is codon optimized

Product Images

Circular map for RG223103

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TNPO3
• Synonyms: IPO12; LGMD1F; LGMDD2; MTR10A; TRN-SR; TRN-SR2; TRNSR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223103 ORF sequence, codon optimized.
  Due to the complexity of NM_012470, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGAGCAAAGCCGACATTGCAGCTCGTGTACCAGGCAGTGCAGGCGCTTTACCACGACCCAGATC
  CCAGCGGAAAGGAGCGCGCCTCTTTTTGGCTTGGGGAGCTGCAGCGTTCGGTTCATGCATGGGAGATCTC
  AGACCAGTTGTTACAGATCCGGCAGGATGTGGAGTCATGCTATTTTGCTGCACAGACCATGAAAATGAAG
  ATTCAGACCTCATTTTATGAGCTCCCCACAGACTCTCATGCCTCTTTACGGGACTCATTGCTAACCCATA
  TCCAGAACTTGAAAGACTTGTCACCTGTTATTGTAACGCAGCTGGCTTTAGCAATAGCAGATCTTGCCCT
  ACAGATGCCTTCCTGGAAGGGATGTGTGCAAACACTGGTGGAAAAATACAGCAATGATGTGACTTCTTTG
  CCTTTTTTGCTGGAGATCCTTACAGTGTTACCTGAAGAAGTACATAGTCGTTCCTTACGAATTGGAGCTA
  ATCGGCGCACAGAAATTATAGAAGATTTGGCCTTCTACTCTAGTACAGTAGTATCTCTATTGATGACCTG
  TGTAGAAAAAGCAGGAACAGATGAGAAAATGCTTATGAAGGTTTTTCGCTGTTTGGGAAGTTGGTTTAAC
  TTGGGAGTTTTGGACAGTAACTTCATGGCTAACAATAAATTACTAGCACTCCTTTTTGAGGTTTTGCAAC
  AGGATAAGACCTCGTCTAACCTACATGAAGCTGCTTCGGACTGTGTATGCTCAGCTCTCTATGCCATTGA
  GAATGTGGAGACTAACTTGCCATTAGCCATGCAACTTTTTCAGGGAGTGCTGACATTGGAGACTGCCTAT
  CATATGGCCGTGGCACGTGAAGATTTAGACAAAGTTCTGAATTACTGCCGTATTTTCACTGAACTATGTG
  AAACTTTTCTTGAAAAAATTGTTTGTACTCCAGGCCAAGGTCTTGGGGACCTTCGAACTCTGGAGCTGCT
  GCTTATCTGTGCAGGCCATCCTCAATATGAGGTAGTAGAAATTTCATTTAACTTTTGGTACCGACTGGGG
  GAACATTTGTACAAAACTAACGATGAAGTTATTCATGGCATCTTCAAAGCTTACATTCAGAGGCTGCTTC
  ACGCCTTGGCTCGACACTGCCAGCTGGAACCAGACCATGAGGGGGTTCCTGAGGAGACTGATGACTTTGG
  GGAGTTTCGCATGAGGGTATCAGACCTGGTAAAGGACTTGATTTTCTTGATAGGGTCTATGGAGTGTTTT
  GCTCAGTTATATTCTACTCTGAAAGAAGGCAACCCACCCTGGGAGGTGACAGAAGCGGTTCTCTTTATCA
  TGGCTGCTATAGCAAAGAGTGTTGATCCGGAAAACAATCCAACACTTGTGGAAGTCCTAGAAGGAGTTGT
  CCGCCTCCCGGAGACCGTACATACGGCTGTGCGATACACCAGCATTGAATTGGTTGGAGAGATGAGTGAA
  GTCGTTGATCGAAATCCTCAGTTCCTTGACCCTGTGTTGGGCTATTTGATGAAAGGCCTGTGTGAAAAGC
  CCCTGGCTTCTGCTGCAGCCAAAGCCATTCATAACATTTGCTCTGTCTGCCGAGATCACATGGCTCAGCA
  CTTTAATGGACTCCTGGAGATTGCCCGCTCCCTCGATTCCTTCCTGTTGTCTCCAGAAGCTGCTGTGGGC
  TTGCTAAAAGGGACAGCACTTGTCCTAGCCCGATTACCTTTGGATAAGATTACCGAATGTCTTAGTGAAC
  TATGTTCTGTTCAGGTTATGGCATTGAAAAAGCTGTTGTCTCAAGAGCCCAGCAATGGCATATCCTCAGA
  TCCCACAGTGTTCTTAGATCGCCTTGCAGTGATATTTAGGCATACCAATCCCATTGTGGAAAATGGACAG
  ACTCATCCGTGTCAGAAAGTCATACAGGAAATATGGCCAGTTTTATCCGAGACTCTAAATAAGCACCGAG
  CTGATAATCGGATTGTAGAGCGTTGTTGCAGGTGCCTGCGCTTTGCTGTTCGCTGTGTAGGCAAAGGATC
  TGCAGCACTGCTGCAGCCACTAGTCACACAGATGGTGAATGTGTACCACGTACATCAGCATTCCTGCTTC
  CTGTACCTTGGCAGTATCCTTGTGGATGAATATGGCATGGAAGAAGGCTGTCGGCAGGGACTGCTAGACA
  TGCTCCAGGCACTGTGCATCCCCACCTTTCAGCTCCTAGAACAGCAGAATGGTCTCCAGAATCACCCTGA
  CACTGTAGATGACCTGTTCCGGCTAGCCACCAGGTTTATTCAGCGTAGCCCTGTCACCTTGCTGCGGAGC
  CAAGTGGTCATCCCTATCTTACAGTGGGCCATTGCCTCTACTACCCTGGACCACCGGGATGCCAATTGTA
  GTGTCATGAGGTTTCTACGAGACCTCATTCATACAGGGGTAGCCAATGATCATGAAGAAGACTTTGAATT
  ACGGAAAGAACTGATTGGACAGGTGATGAACCAGCTTGGACAGCAGCTTGTCAGCCAGCTGCTGCACACC
  TGCTGCTTTTGCCTCCCCCCCTATACCCTACCAGATGTGGCTGAAGTGCTCTGGGAGATCATGCAGGTTG
  ACAGACCGACTTTTTGTCGATGGTTAGAAAATTCCTTAAAAGGTTTGCCAAAGGAAACAACCGTGGGAGC
  CGTCACAGTGACACACAAACAACTTACAGACTTCCACAAGCAAGTCACTAGTGCTGAGGAATGTAAACAA
  GTTTGCTGGGCCTTGCGAGACTTCACCAGGTTGTTTCGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223103 representing NM_012470
  Red=Cloning site Green=Tags(s)
  
  MEGAKPTLQLVYQAVQALYHDPDPSGKERASFWLGELQRSVHAWEISDQLLQIRQDVESCYFAAQTMKMK
  IQTSFYELPTDSHASLRDSLLTHIQNLKDLSPVIVTQLALAIADLALQMPSWKGCVQTLVEKYSNDVTSL
  PFLLEILTVLPEEVHSRSLRIGANRRTEIIEDLAFYSSTVVSLLMTCVEKAGTDEKMLMKVFRCLGSWFN
  LGVLDSNFMANNKLLALLFEVLQQDKTSSNLHEAASDCVCSALYAIENVETNLPLAMQLFQGVLTLETAY
  HMAVAREDLDKVLNYCRIFTELCETFLEKIVCTPGQGLGDLRTLELLLICAGHPQYEVVEISFNFWYRLG
  EHLYKTNDEVIHGIFKAYIQRLLHALARHCQLEPDHEGVPEETDDFGEFRMRVSDLVKDLIFLIGSMECF
  AQLYSTLKEGNPPWEVTEAVLFIMAAIAKSVDPENNPTLVEVLEGVVRLPETVHTAVRYTSIELVGEMSE
  VVDRNPQFLDPVLGYLMKGLCEKPLASAAAKAIHNICSVCRDHMAQHFNGLLEIARSLDSFLLSPEAAVG
  LLKGTALVLARLPLDKITECLSELCSVQVMALKKLLSQEPSNGISSDPTVFLDRLAVIFRHTNPIVENGQ
  THPCQKVIQEIWPVLSETLNKHRADNRIVERCCRCLRFAVRCVGKGSAALLQPLVTQMVNVYHVHQHSCF
  LYLGSILVDEYGMEEGCRQGLLDMLQALCIPTFQLLEQQNGLQNHPDTVDDLFRLATRFIQRSPVTLLRS
  QVVIPILQWAIASTTLDHRDANCSVMRFLRDLIHTGVANDHEEDFELRKELIGQVMNQLGQQLVSQLLHT
  CCFCLPPYTLPDVAEVLWEIMQVDRPTFCRWLENSLKGLPKETTVGAVTVTHKQLTDFHKQVTSAEECKQ
  VCWALRDFTRLFR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012470
• ORF Size: 2769 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012470.4
• RefSeq Size: 4422 bp
• RefSeq ORF: 2772 bp
• Locus ID: 23534
• UniProt ID: Q9Y5L0
• Cytogenetics: 7q32.1
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a nuclear import receptor for serine/arginine-rich (SR) proteins such as the splicing factors SFRS1 and SFRS2. The encoded protein has also been shown to be involved in HIV-1 infection, apparently through interaction with the HIV-1 capsid protein. Several protein-coding and non-coding transcript variants have been found for this gene. [provided by RefSeq, Apr 2020]