BIG1 (ARFGEF1) (NM_006421) Human Tagged ORF Clone

CAT#: RG222817

ARFGEF1 (tGFP-tagged) - Human ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (ARFGEF1)

Product Images

Circular map for RG222817

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BIG1
• Synonyms: ARFGEP1; BIG1; P200
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222817 representing NM_006421
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGAGGGGAAGAAGACGAAGAACATGTTCCTGACCCGGGCTCTGGAGAAGATATTGGCCGACAAGG
  AAGTGAAGAAGGCGCATCACTCCCAGCTGCGCAAAGCTTGCGAGGTGGCGTTAGAGGAAATAAAAGCGGA
  AACTGAAAAACAGAGTCCTCCTCATGGAGAAGCAAAAGCTGGATCAAGCACCCTTCCACCAGTGAAATCA
  AAGACAAATTTTATTGAAGCAGACAAGTACTTCTTGCCTTTTGAGTTGGCATGCCAGTCCAAATGTCCTC
  GCATAGTTAGTACATCTCTAGATTGCTTACAGAAACTTATTGCTTATGGGCACTTGACTGGCAATGCTCC
  AGATAGTACAACACCAGGCAAAAAATTAATTGATAGAATTATTGAAACAATATGTGGCTGCTTTCAGGGC
  CCTCAGACAGATGAAGGAGTTCAGCTGCAGATAATAAAGGCTTTACTTACTGCAGTAACATCACAACACA
  TAGAAATTCATGAAGGGACTGTACTGCAAGCTGTGAGAACATGTTACAATATCTACTTAGCAAGCAAAAA
  TCTCATCAATCAGACAACAGCCAAAGCTACTCTCACTCAGATGCTAAATGTTATCTTTGCACGCATGGAA
  AACCAAGCATTACAGGAAGCAAAACAAATGGAAAAAGAAAGGCATCGGCAGCATCATCATCTGTTACAGT
  CTCCAGTAAGCCATCACGAGCCTGAATCACCTCAACTTAGATATTTGCCACCTCAGACTGTTGATCATAT
  ATCCCAAGAACACGAAGGGGACCTTGACCTCCATACAAATGATGTAGATAAAAGTCTTCAGGATGACACA
  GAGCCTGAAAATGGATCTGATATTTCCAGTGCAGAAAATGAACAGACTGAAGCTGATCAGGCAACTGCAG
  CTGAAACATTATCTAAAAATGAAGTGTTATATGACGGAGAAAACCATGATTGTGAGGAAAAGCCACAAGA
  CATTGTACAGAACATTGTAGAAGAAATGGTGAACATTGTTGTTGGAGATATGGGAGAAGGGACTACTATA
  AATGCAAGTGCAGATGGCAACATTGGAACTATAGAGGATGGTAGTGACAGTGAAAATATTCAAGCAAATG
  GAATTCCAGGAACACCAATTTCTGTTGCATATACACCATCCTTACCTGATGATAGATTGTCAGTCTCTTC
  CAATGATACTCAGGAATCTGGAAATTCTTCAGGACCTTCACCTGGTGCTAAGTTTTCCCACATTTTACAA
  AAGGATGCCTTTCTAGTATTCAGGTCATTGTGTAAACTGTCAATGAAACCACTGTCAGATGGACCACCAG
  ATCCAAAGTCTCATGAACTACGATCCAAGATTCTTTCATTGCAGTTACTTCTATCCATTCTGCAGAATGC
  AGGACCTATTTTCAGGACAAATGAGATGTTTATTAATGCTATTAAGCAGTATCTTTGTGTTGCACTCTCA
  AAAAATGGAGTCTCATCTGTTCCAGAGGTTTTTGAGCTTTCTCTTTCTATATTTCTTACTTTGTTGTCAA
  ATTTCAAGACACATCTGAAGATGCAAATTGAGGTGTTCTTTAAAGAAATTTTCTTATACATTTTGGAAAC
  TTCTACCAGCTCATTTGATCACAAATGGATGGTTATTCAGACACTGACGAGGATTTGTGCAGATGCTCAG
  AGTGTAGTGGATATTTATGTAAACTATGACTGTGACTTAAATGCAGCCAATATATTTGAAAGACTAGTAA
  ATGATCTATCAAAAATTGCTCAAGGAAGGGGCAGTCAAGAACTTGGTATGAGTAATGTTCAGGAATTGAG
  CCTGAGGAAAAAAGGTTTAGAATGCTTAGTGTCGATTTTGAAGTGTATGGTTGAATGGAGTAAGGATCAG
  TATGTGAATCCCAACTCCCAGACAACTCTTGGTCAGGAAAAACCCTCAGAGCAAGAGATGAGTGAAATCA
  AACACCCTGAGACAATAAACAGATACGGAAGTTTAAATTCCCTGGAGTCAACATCATCATCAGGAATAGG
  CAGCTACAGTACACAGATGTCTGGCACTGATAATCCAGAACAATTTGAGGTCCTAAAGCAACAAAAAGAA
  ATAATAGAACAAGGGATAGATTTATTTAATAAGAAACCAAAGAGAGGAATACAGTACCTCCAAGAACAAG
  GGATGCTTGGCACCACACCTGAAGATATTGCCCAATTCTTACATCAAGAGGAAAGATTAGACTCTACTCA
  AGTGGGTGAGTTCCTGGGAGATAATGATAAATTTAACAAAGAAGTCATGTATGCATATGTGGACCAACAT
  GACTTTTCAGGAAAAGACTTCGTTTCAGCCCTTCGTATGTTTCTAGAAGGATTTCGTCTTCCAGGGGAAG
  CTCAGAAAATCGATCGATTAATGGAAAAATTTGCTGCAAGATACCTAGAATGCAACCAAGGACAAACTCT
  CTTTGCTAGTGCGGATACAGCTTATGTTTTGGCTTATTCAATTATCATGTTGACCACAGACCTTCACAGT
  CCACAGGTGAAAAATAAAATGACAAAGGAACAATACATTAAGATGAATAGAGGTATCAATGACAGTAAAG
  ACCTTCCTGAAGAGTATCTATCAGCCATCTATAATGAAATAGCTGGGAAAAAGATATCAATGAAAGAAAC
  AAAAGAACTAACAATCCCTACAAAATCAAGTAAACAGAATGTAGCCAGTGAAAAACAAAGAAGACTTCTG
  TATAACTTAGAAATGGAGCAGATGGCCAAGACAGCTAAAGCACTCATGGAGGCCGTGAGCCATGTTCAGG
  CACCCTTTACAAGTGCAACACATTTGGAGCATGTGAGGCCCATGTTTAAGTTGGCTTGGACGCCTTTTCT
  GGCTGCATTCAGTGTGGGTCTACAAGATTGTGATGATACTGAAGTAGCCTCTCTTTGCCTGGAAGGTATA
  AGATGTGCAATCAGAATTGCATGCATTTTCAGCATTCAGCTGGAGAGAGATGCATATGTCCAGGCACTAG
  CAAGATTTACCTTACTCACAGTGAGTTCCGGTATTACTGAAATGAAACAGAAGAACATTGACACAATAAA
  AACACTTATCACAGTGGCTCATACAGATGGAAATTATTTAGGAAATTCATGGCATGAGATTCTGAAGTGC
  ATCAGTCAGTTGGAGCTGGCACAGCTTATAGGAACTGGAGTGAAACCTCGATACATTTCTGGAACAGTGC
  GAGGCAGAGAAGGATCTCTTACTGGAACAAAAGATCAGGCTCCTGATGAATTTGTGGGTTTAGGGCTAGT
  TGGAGGAAATGTGGACTGGAAACAGATAGCAAGTATTCAGGAATCCATTGGAGAAACCAGTTCTCAGAGT
  GTTGTTGTTGCTGTAGATAGAATATTCACAGGATCTACAAGGCTAGATGGAAATGCCATTGTGGATTTTG
  TCCGTTGGCTCTGTGCTGTGTCTATGGATGAATTACTTTCCACGACACACCCAAGAATGTTTAGTCTACA
  AAAAATAGTAGAAATATCATATTACAACATGGGAAGAATAAGATTACAGTGGTCTCGAATTTGGGAAGTT
  ATTGGAGATCATTTTAATAAGGTTGGGTGTAATCCTAATGAAGATGTAGCTATTTTTGCAGTAGACTCCT
  TGAGGCAGTTGTCAATGAAGTTCTTAGAGAAAGGGGAGCTTGCTAACTTCAGATTCCAGAAGGATTTCTT
  AAGACCTTTTGAACATATAATGAAACGGAACAGGTCTCCAACAATTCGAGATATGGTTGTACGGTGTATA
  GCACAGATGGTTAATTCTCAAGCTGCTAACATTCGATCTGGATGGAAGAACATTTTCTCTGTATTTCATC
  TAGCTGCATCTGATCAAGATGAAAGCATAGTGGAACTTGCATTCCAAACAACCGGGCACATTGTCACCCT
  TGTATTTGAAAAACACTTTCCAGCGACCATTGATTCTTTCCAGGATGCAGTGAAGTGTTTGTCTGAATTT
  GCGTGCAATGCAGCTTTCCCAGACACAAGTATGGAAGCAATTCGACTTATTCGCCATTGTGCAAAATATG
  TGTCTGATAGACCTCAGGCTTTCAAGGAATACACAAGCGATGATATGAACGTAGCACCTGAAGACAGGGT
  GTGGGTGAGAGGATGGTTCCCAATTCTCTTTGAGTTATCCTGTATCATCAATAGATGCAAATTAGATGTA
  AGAACCAGGGGTTTAACAGTAATGTTTGAAATAATGAAAACATATGGCCACACTTATGAGAAACACTGGT
  GGCAGGATTTATTTAGAATTGTTTTCAGAATCTTTGACAATATGAAATTGCCAGAACAACAGACAGAGAA
  AGCTGAATGGATGACAACAACTTGCAATCATGCACTTTATGCAATCTGTGATGTATTCACTCAGTATTTA
  GAAGTACTCAGTGATGTACTTTTGGATGACATTTTTGCTCAGCTCTACTGGTGTGTGCAGCAAGACAATG
  AGCAGTTAGCGCGATCTGGTACAAACTGTTTAGAGAATGTTGTTATTCTGAATGGTGAAAAATTTACCCT
  AGAAATCTGGGATAAAACTTGCAACTGCACACTGGATATCTTCAAAACCACAATCCCACATGCGCTGTTG
  ACCTGGCGACCCAATTCTGGAGAAACTGCCCCCCCACCTCCATCTCCTGTAAGTGAAAAGCCATTGGATA
  CAATATCACAGAAGTCTGTAGATATTCATGATTCTATTCAACCAAGGTCTGTGGATAACAGACCACAAGC
  ACCACTGGTTTCTGCGTCTGCTGTTAATGAAGAAGTCAGCAAAATTAAATCTACAGCAAAATTTCCAGAA
  CAAAAATTGTTTGCTGCCCTGTTGATTAAATGTGTTGTGCAGCTGGAACTCATCCAGACTATCGACAACA
  TTGTCTTCTTCCCAGCCACAAGTAAGAAAGAAGATGCAGAAAACTTAGCTGCAGCACAGAGAGATGCGGT
  GGACTTTGATGTTCGCGTTGATACTCAAGACCAAGGAATGTACCGCTTTTTAACATCACAACAACTTTTT
  AAGCTACTGGACTGCTTATTAGAGTCACATAGATTTGCAAAAGCGTTTAATTCCAACAACGAACAGAGGA
  CTGCCCTGTGGAAAGCAGGATTCAAAGGCAAATCCAAGCCCAACCTTCTGAAGCAGGAGACCAGCAGCCT
  GGCCTGTGGGCTGCGCATTCTCTTCCGGATGTACATGGATGAGAGCCGCGTTAGTGCCTGGGAGGAGGTC
  CAGCAGAGGCTTTTGAATGTCTGCAGTGAAGCACTAAGTTACTTCCTCACTCTAACATCAGAAAGTCATC
  GAGAAGCCTGGACTAACTTACTGCTTTTGTTTCTAACTAAAGTTCTAAAGATAAGTGATAATAGGTTTAA
  AGCTCATGCATCATTCTACTACCCTCTCTTATGTGAAATTATGCAATTTGACTTGATTCCTGAACTTCGT
  GCTGTTCTTAGAAGATTTTTTCTGCGAATCGGAGTAGTTTTTCAGATATCACAACCACCTGAACAGGAAC
  TTGGAATAAACAAGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222817 representing NM_006421
  Red=Cloning site Green=Tags(s)
  
  MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKS
  KTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQG
  PQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARME
  NQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDT
  EPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTI
  NASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQ
  KDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALS
  KNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQ
  SVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQ
  YVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKE
  IIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQH
  DFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHS
  PQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLL
  YNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGI
  RCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKC
  ISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQS
  VVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEV
  IGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCI
  AQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEF
  ACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDV
  RTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYL
  EVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALL
  TWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPE
  QKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLF
  KLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEV
  QQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELR
  AVLRRFFLRIGVVFQISQPPEQELGINKQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006421
• ORF Size: 5547 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006421.5
• RefSeq Size: 7212 bp
• RefSeq ORF: 5550 bp
• Locus ID: 10565
• UniProt ID: Q9Y6D6
• Cytogenetics: 8q13.2
• Domains: Sec7
• Gene Summary: ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Aug 2011]