TRP 7 (TRPC7) (NM_020389) Human Tagged ORF Clone

CAT#: RG222755

TRPC7 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 7 (TRPC7), transcript variant 1

Product Images

Circular map for RG222755

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRP 7
• Synonyms: TRP7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222755 representing NM_020389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAGGAACAGCACCTTCAAAAACATGCAGCGCCGGCACACAACGCTGAGGGAGAAGGGCCGTCGCC
  AGGCCATCCGGGGTCCCGCCTACATGTTCAACGAGAAGGGCACCAGTCTGACGCCCGAGGAGGAGCGCTT
  CCTGGACTCGGCTGAGTATGGCAACATCCCGGTGGTCCGGAAAATGCTGGAGGAGTCCAAGACCCTTAAC
  TTCAACTGTGTGGACTACATGGGGCAGAACGCTCTGCAGCTGGCCGTGGGCAACGAGCACCTAGAGGTCA
  CGGAGCTGCTGCTGAAGAAGGAGAACCTGGCACGGGTGGGGGACGCGCTGCTGCTGGCCATCAGCAAGGG
  CTATGTGCGCATCGTGGAGGCCATCCTCAACCACCCGGCCTTCGCGCAGGGCCAGCGCCTGACGCTCAGC
  CCGCTGGAACAGGAGCTGCGCGACGACGACTTCTATGCCTACGACGAGGACGGCACGCGCTTCTCCCACG
  ACATCACGCCCATCATCCTGGCGGCGCACTGCCAGGAGTATGAGATCGTGCACATCCTGCTGCTCAAGGG
  CGCCCGCATCGAGCGGCCCCACGACTACTTCTGCAAGTGCAATGAGTGCACCGAGAAACAGCGGAAAGAC
  TCCTTCAGCCACTCGCGCTCGCGCATGAACGCCTACAAAGGACTGGCGAGTGCTGCCTACTTGTCCCTGT
  CCAGCGAAGACCCTGTCCTCACCGCCCTGGAGCTCAGCAACGAGTTAGCCAGACTAGCCAACATTGAGAC
  TGAATTTAAGAACGATTACAGGAAGTTATCTATGCAATGCAAGGATTTTGTAGTGGGCGTGCTGGACCTG
  TGCCGAGACACAGAAGAGGTGGAAGCAATTTTAAACGGTGATGTGAACTTCCAAGTCTGGTCCGACCACC
  ACCGTCCAAGTCTGAGCCGGATCAAACTCGCCATTAAATATGAAGTCAAGAAGTTCGTTGCTCATCCTAA
  CTGTCAGCAGCAATTGCTTACCATGTGGTATGAAAATCTCTCAGGCTTACGTCAACAGTCTATCGCTGTG
  AAATTCCTGGCTGTCTTTGGAGTCTCCATAGGCCTCCCTTTTCTCGCCATAGCCTATTGGATTGCTCCGT
  GCAGCAAGCTAGGACGAACCCTGAGGAGCCCTTTCATGAAGTTTGTAGCTCATGCAGTTTCTTTTACAAT
  CTTCTTGGGATTATTAGTTGTGAATGCATCTGACCGATTTGAAGGTGTTAAAACCCTGCCAAACGAAACC
  TTCACAGACTACCCAAAACAAATCTTCAGAGTGAAAACCACACAGTTCTCCTGGACAGAAATGCTCATTA
  TGAAGTGGGTCTTAGGAATGATTTGGTCCGAATGCAAGGAAATCTGGGAGGAGGGGCCACGGGAGTACGT
  GCTGCACTTGTGGAACCTGCTAGATTTCGGGATGCTGTCCATCTTCGTGGCCTCCTTCACAGCACGCTTC
  ATGGCCTTCCTGAAGGCCACGGAGGCACAGCTGTACGTGGACCAGCACGTGCAGGACGACACGCTGCACA
  ATGTCTCGCTTCCGCCGGAAGTGGCATACTTCACCTACGCCAGGGACAAGTGGTGGCCTTCAGACCCTCA
  GATCATATCGGAAGGGCTCTACGCGATAGCCGTCGTGCTGAGCTTCTCTCGCATTGCATACATTCTGCCA
  GCCAACGAGAGTTTTGGGCCCCTGCAGATCTCGCTAGGGAGAACTGTGAAAGATATCTTCAAGTTCATGG
  TCATTTTCATCATGGTATTTGTGGCCTTCATGATTGGGATGTTCAACCTGTACTCTTACTACCGAGGTGC
  CAAATACAACCCAGCGTTTACAACGGTTGAAGAAAGTTTTAAAACTTTGTTTTGGTCCATATTCGGCTTA
  TCTGAAGTAATCTCAGTGGTGCTGAAATACGACCACAAATTCATCGAGAACATTGGCTACGTTCTCTACG
  GCGTTTATAACGTCACCATGGTGGTAGTGTTGCTCAACATGCTAATAGCCATGATAAACAACTCCTATCA
  GGAAATTGAGGAGGATGCAGATGTGGAATGGAAGTTCGCCCGAGCAAAACTCTGGCTGTCTTACTTTGAT
  GAAGGAAGAACTCTACCTGCTCCTTTTAATCTAGTGCCAAGTCCTAAATCATTTTATTATCTCATAATGA
  GAATCAAGATGTGCCTCATAAAACTCTGCAAATCTAAGGCCAAAAGCTGTGAAAATGACCTTGAAATGGG
  CATGCTGAATTCCAAATTCAAGAAGACTCGCTACCAGGCTGGCATGAGGAATTCTGAAAATCTGACAGCA
  AATAACACTTTGAGCAAGCCCACCAGATACCAGAAAATCATGAAACGGCTCATAAAAAGATACGTCCTGA
  AAGCCCAGGTGGACAGAGAAAATGACGAAGTCAATGAAGGCGAGCTGAAGGAAATCAAGCAAGATATCTC
  CAGCCTGCGCTATGAGCTTCTTGAGGAAAAATCTCAAGCTACTGGTGAGCTGGCAGACCTGATTCAACAA
  CTCAGCGAGAAGTTTGGAAAGAACTTAAACAAAGACCACCTGAGGGTGAACAAGGGCAAAGACATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222755 representing NM_020389
  Red=Cloning site Green=Tags(s)
  
  MLRNSTFKNMQRRHTTLREKGRRQAIRGPAYMFNEKGTSLTPEEERFLDSAEYGNIPVVRKMLEESKTLN
  FNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARVGDALLLAISKGYVRIVEAILNHPAFAQGQRLTLS
  PLEQELRDDDFYAYDEDGTRFSHDITPIILAAHCQEYEIVHILLLKGARIERPHDYFCKCNECTEKQRKD
  SFSHSRSRMNAYKGLASAAYLSLSSEDPVLTALELSNELARLANIETEFKNDYRKLSMQCKDFVVGVLDL
  CRDTEEVEAILNGDVNFQVWSDHHRPSLSRIKLAIKYEVKKFVAHPNCQQQLLTMWYENLSGLRQQSIAV
  KFLAVFGVSIGLPFLAIAYWIAPCSKLGRTLRSPFMKFVAHAVSFTIFLGLLVVNASDRFEGVKTLPNET
  FTDYPKQIFRVKTTQFSWTEMLIMKWVLGMIWSECKEIWEEGPREYVLHLWNLLDFGMLSIFVASFTARF
  MAFLKATEAQLYVDQHVQDDTLHNVSLPPEVAYFTYARDKWWPSDPQIISEGLYAIAVVLSFSRIAYILP
  ANESFGPLQISLGRTVKDIFKFMVIFIMVFVAFMIGMFNLYSYYRGAKYNPAFTTVEESFKTLFWSIFGL
  SEVISVVLKYDHKFIENIGYVLYGVYNVTMVVVLLNMLIAMINNSYQEIEEDADVEWKFARAKLWLSYFD
  EGRTLPAPFNLVPSPKSFYYLIMRIKMCLIKLCKSKAKSCENDLEMGMLNSKFKKTRYQAGMRNSENLTA
  NNTLSKPTRYQKIMKRLIKRYVLKAQVDRENDEVNEGELKEIKQDISSLRYELLEEKSQATGELADLIQQ
  LSEKFGKNLNKDHLRVNKGKDI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020389
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020389.3
• RefSeq Size: 2589 bp
• RefSeq ORF: 2589 bp
• Locus ID: 57113
• UniProt ID: Q9HCX4
• Cytogenetics: 5q31.1
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) (By similarity). May also be activated by intracellular calcium store depletion.[UniProtKB/Swiss-Prot Function]