PPP1R10 (NM_002714) Human Tagged ORF Clone

CAT#: RG221507

PPP1R10 (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10)

Product Images

Circular map for RG221507

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPP1R10
• Synonyms: CAT53; FB19; p99; PNUTS; PP1R10; R111
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221507 representing NM_002714
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCGGGTCCCATAGACCCCAAAGAACTTCTCAAGGGCCTGGACAGCTTCCTTAACCGAGATGGGG
  AAGTCAAAAGTGTGGATGGGATTTCCAAGATCTTCAGTTTGATGAAGGAAGCACGAAAGATGGTGAGTCG
  ATGCACTTACTTGAACATTCTCCTGCAGACCCGTTCACCAGAAATATTGGTCAAATTTATTGACGTTGGC
  GGCTACAAACTTCTTAACAATTGGCTGACGTATTCAAAGACAACCAACAACATTCCCCTCCTCCAGCAAA
  TTCTACTGACCCTGCAGCATCTACCGCTCACTGTAGACCATCTCAAGCAGAACAACACAGCTAAACTGGT
  GAAGCAGCTGAGCAAGTCAAGTGAGGATGAAGAGCTCCGGAAATTGGCCTCAGTCCTTGTCAGCGACTGG
  ATGGCTGTCATCCGCTCTCAGAGCAGTACCCAGCCTGCTGAGAAAGATAAGAAGAAACGTAAAGATGAAG
  GAAAAAGTCGAACTACCCTTCCTGAGCGACCTTTGACAGAGGTGAAGGCTGAGACCCGGGCTGAGGAGGC
  CCCAGAGAAGAAGAGGGAGAAGCCCAAGTCTCTTCGCACCACAGCACCCAGTCATGCCAAGTTCCGTTCC
  ACTGGACTAGAGCTGGAGACACCATCCTTGGTGCCTGTGAAGAAGAATGCCAGCACAGTGGTGGTTTCTG
  ACAAGTACAACCTTAAACCCATCCCCCTCAAACGTCAGAGCAACGTAGCTGCTCCAGGAGATGCCACTCC
  CCCTGCAGAGAAGAAATACAAGCCACTCAACACAACACCTAATGCCACCAAAGAGATCAAAGTGAAGATC
  ATCCCGCCACAGCCTATGGAGGGCCTGGGCTTTCTGGATGCTCTTAATTCAGCCCCTGTTCCAGGCATCA
  AAATTAAGAAGAAAAAAAAAGTACTGTCACCTACGGCTGCCAAGCCAAGCCCCTTTGAAGGGAAAACGAG
  CACAGAACCAAGCACAGCCAAACCTTCTTCCCCAGAACCAGCACCACCTTCTGAGGCAATGGACGCAGAC
  CGTCCAGGCACCCCGGTTCCCCCTGTTGAAGTCCCGGAGCTCATGGATACAGCCTCTTTGGAGCCAGGAG
  CTCTGGATGCCAAGCCAGTGGAGAGTCCTGGAGATCCTAACCAACTGACCCGGAAAGGCAGGAAGAGGAA
  AAGTGTGACATGGCCTGAGGAAGGCAAACTGAGAGAATATTTCTATTTTGAATTGGATGAAACTGAACGA
  GTAAATGTGAATAAGATCAAGGACTTTGGTGAGGCGGCTAAGCGAGAGATACTGTCAGACCGACATGCAT
  TTGAGACAGCGCGGCGTCTGAGCCATGATAACATGGAGGAGAAGGTGCCCTGGGTGTGCCCCCGGCCCCT
  GGTTCTGCCCTCACCTCTTGTCACCCCTGGAAGCAATAGTCAGGAGCGATATATCCAGGCTGAGCGGGAG
  AAGGGAATCCTTCAGGAGCTCTTCCTGAACAAGGAGAGTCCTCATGAGCCTGATCCTGAGCCCTACGAGC
  CCATACCCCCTAAACTCATCCCCCTAGATGAGGAGTGTTCCATGGATGAGACTCCGTATGTTGAGACTCT
  GGAACCTGGGGGGTCAGGTGGCTCACCTGATGGGGCAGGAGGCTCCAAGTTGCCTCCAGTTCTGGCCAAT
  CTTATGGGAAGCATGGGTGCTGGAAAGGGCCCCCAAGGCCCTGGAGGAGGAGGCATTAATGTCCAAGAGA
  TCCTCACCTCCATCATGGGTAGCCCAAACAGTCATCCTTCAGAGGAACTACTGAAACAACCAGACTATTC
  GGACAAGATCAAGCAGATGCTGGTGCCACATGGACTCCTAGGCCCTGGCCCAATAGCCAATGGTTTCCCA
  CCAGGGGGTCCTGGGGGCCCCAAGGGCATGCAGCACTTTCCCCCTGGACCTGGGGGACCTATGCCAGGTC
  CCCATGGAGGCCCTGGTGGGCCAGTGGGTCCACGTCTTCTGGGTCCTCCACCCCCTCCCCGGGGAGGTGA
  TCCCTTCTGGGATGGCCCGGGCGACCCTATGCGGGGTGGCCCAATGCGGGGGGGTCCAGGACCAGGTCCT
  GGACCATACCATAGAGGCCGAGGTGGCCGAGGAGGAAACGAACCTCCTCCTCCTCCTCCTCCATTCCGAG
  GCGCCAGAGGAGGTCGCTCTGGAGGAGGACCCCCAAATGGACGAGGGGGCCCTGGTGGGGGCATGGTTGG
  AGGTGGTGGGCATCGTCCTCACGAAGGCCCTGGTGGGGGCATGGGCAACAGCAGTGGACATCGTCCCCAC
  GAAGGCCCTGGCGGTGGCATGGGAAGTGGGCATCGCCCCCATGAAGGCCCTGGTGGTAGCATGGGTGGGG
  GTGGAGGACATCGTCCCCACGAAGGCCCTGGCGGTGGCATCAGTGGTGGCAGTGGCCATCGTCCCCATGA
  AGGCCCTGGCGGAGGAATGGGTGCCGGTGGTGGACATCGCCCCCACGAAGGCCCTGGCGGAAGCATGGGT
  GGAAGTGGTGGACATCGTCCCCATGAAGGCCCTGGACACGGGGGGCCCCATGGCCACCGGCCTCATGATG
  TCCCTGGTCACCGAGGCCATGACCATCGAGGGCCGCCACCTCATGAGCACCGTGGCCATGATGGTCCTGG
  CCACGGGGGAGGGGGCCACCGAGGGCACGATGGAGGCCACAGCCATGGAGGAGACATGTCAAACCGCCCT
  GTCTGCCGACATTTCATGATGAAGGGCAACTGCCGCTATGAGAACAACTGTGCCTTCTACCACCCGGGTG
  TCAATGGGCCCCCCCTGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221507 representing NM_002714
  Red=Cloning site Green=Tags(s)
  
  MGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNILLQTRSPEILVKFIDVG
  GYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDW
  MAVIRSQSSTQPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRTTAPSHAKFRS
  TGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKI
  IPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAKPSPFEGKTSTEPSTAKPSSPEPAPPSEAMDAD
  RPGTPVPPVEVPELMDTASLEPGALDAKPVESPGDPNQLTRKGRKRKSVTWPEEGKLREYFYFELDETER
  VNVNKIKDFGEAAKREILSDRHAFETARRLSHDNMEEKVPWVCPRPLVLPSPLVTPGSNSQERYIQAERE
  KGILQELFLNKESPHEPDPEPYEPIPPKLIPLDEECSMDETPYVETLEPGGSGGSPDGAGGSKLPPVLAN
  LMGSMGAGKGPQGPGGGGINVQEILTSIMGSPNSHPSEELLKQPDYSDKIKQMLVPHGLLGPGPIANGFP
  PGGPGGPKGMQHFPPGPGGPMPGPHGGPGGPVGPRLLGPPPPPRGGDPFWDGPGDPMRGGPMRGGPGPGP
  GPYHRGRGGRGGNEPPPPPPPFRGARGGRSGGGPPNGRGGPGGGMVGGGGHRPHEGPGGGMGNSSGHRPH
  EGPGGGMGSGHRPHEGPGGSMGGGGGHRPHEGPGGGISGGSGHRPHEGPGGGMGAGGGHRPHEGPGGSMG
  GSGGHRPHEGPGHGGPHGHRPHDVPGHRGHDHRGPPPHEHRGHDGPGHGGGGHRGHDGGHSHGGDMSNRP
  VCRHFMMKGNCRYENNCAFYHPGVNGPPLP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002714
• ORF Size: 2820 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002714.4
• RefSeq Size: 4540 bp
• RefSeq ORF: 2823 bp
• Locus ID: 5514
• UniProt ID: Q96QC0
• Cytogenetics: 6p21.33
• Domains: zf-CCCH, TFS2N
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Gene Summary: This gene encodes a protein phosphatase 1 binding protein. The encoded protein plays a role in many cellular processes including cell cycle progression, DNA repair and apoptosis by regulating the activity of protein phosphatase 1. This gene lies within the major histocompatibility complex class I region on chromosome 6, and alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]