GIMAP8 (NM_175571) Human Tagged ORF Clone

CAT#: RG220758

GIMAP8 (tGFP-tagged) - Human GTPase, IMAP family member 8 (GIMAP8)

Product Images

Circular map for RG220758

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GIMAP8
• Synonyms: IAN-9; IAN6; IAN9; IANT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220758 representing NM_175571
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGCAGAGCTGCCAGATGTCCGAACTGCGGCTCCTCCTCCTGGGAAAATGCCGCTCGGGAAAAA
  GTGCCACAGGAAATGCCATTCTGGGCAAACATGTGTTCAAGTCCAAGTTCAGTGATCAGACAGTGATCAA
  AATGTGCCAGAGAGAGAGTTGGGTCCTGAGAGAAAGGAAGGTTGTGGTAATTGACACCCCTGACCTTTTC
  TCCTCAATAGCTTGTGCTGAAGACAAGCAACGCAACATCCAACACTGCTTGGAGCTCTCTGCTCCCAGCC
  TCCATGCTCTGCTCTTGGTAATTGCCATCGGCCATTTCACAAGGGAGGATGAGGAAACAGCCAAGGGCAT
  CCAACAAGTGTTTGGAGCTGAAGCCAGGAGGCACATCATTATTGTCTTCACTCGGAAGGATGATTTGGGG
  GATGACTTGCTGCAAGATTTCATTGAAAAAAACAAACCTCTCAAGCAGTTGGTTCAAGACTATGAGGGCC
  GATACTGCATTTTCAACAACAAGACCAATAGTAAGGATGAGCAGATCACCCAGGTGTTGGAGCTCCTTCG
  CAAGGTTGAGTCTTTGGTGAATACGAACGGAGGACCCTATCATGTGAACTTCAAAACTGAAGGCAGCAGG
  TTTCAAGATTGTGTGAATGAAGCTGCATCTCAAGAGGGAGACAAGCCACAGGGCCCAAGGGAAAGGCAGC
  TGCAGTCCACAGGACCCGAGCAGAATCCGGGGACATCAGAACTGACAGTCCTCCTTGTGGGGAAACGCGG
  TGCTGGAAAAAGTGCAGCAGGAAACAGCATTCTGGGGAGGCAGGCCTTTCAGACCGGATTTAGTGAGCAG
  TCAGTAACCCAGAGCTTCTTGTCTGAGAGCAGAAGCTGGAGAAAAAAGAAAGTTTCGATCATTGATGCTC
  CGGACATCTCATCTTTAAAGAACATTGACTCAGAAGTTAGAAAACACATCTGTACAGGCCCCCATGCCTT
  CCTGCTGGTGACACCACTGGGCTTTTACACTAAGAATGATGAGGCAGTGCTGAGCACCATCCAAAACAAT
  TTTGGAGAAAAATTCTTTGAGTACATGATCATACTTCTTACCAGGAAAGAAGATTTAGGGGATCAGGATC
  TAGATACGTTCTTAAGAAACAGCAATAAAGCTCTCTATGGTCTCATCCAGAAGTGTAAAAACAGATATAG
  TGCCTTCAACTACCGGGCAACAGGAGAAGAAGAGCAAAGGCAGGCGGACGAGCTCCTGGAAAAAATTGAG
  AGCATGGTGCATCAGAATGGGAACAAGCATTGTGTTTTCAGAGAAAAAGAAACCCTGAACATTGTCCTTG
  TGGGGAGAAGCGGGACTGGGAAGAGTGCGACCGGGAACTCTATCCTGGGGAGCCTCGTCTTCACCTCTCG
  GCTCCGGGCCCAGCCAGTCACCAAGACCAGCCAGAGTGGCAGGAGGACATGGGACGGACAGGAGGTGGTG
  GTTGTGGACACTCCTTCCTTCAACCAGATGCTGGATGTCGAAAAGGACCCATCCCGGTTAGAAGAGGAGG
  TCAAGCGCTGTTTGTCCTGCTGTGAAAAAGGGGACACATTTTTTGTCCTGGTGTTCCAGCTGGGACGATT
  CACTGAAGAGGACAAAACAGCTGTGGCGAAACTGGAGGCCATCTTTGGAGCAGACTTTACGAAATACGCG
  ATTATGCTGTTCACCCGGAAGGAAGACCTAGGGGCGGGGAATTTGGAAGACTTCATGAAGAACTCAGATA
  ACAAAGCCCTTCGGCGCATTTTTAAAAAGTGTGGGCGGCGAGTTTGTGCTTTTAACAACAAAGAAACAGG
  CCAGGCCCAGGAAACCCAGGTGAAAGCTCTTTTAACAAAGGTCAATGATCTGAGAAAAGAAAGTGGGTGG
  TCCGGGTATCCCCATACACAGGAGAACGTCAGCAAACTAATTAAAAATGTCCAGGAAATGTCCCAAGCCG
  AAAAACTCCTTAAAAATTTAATAGGTATTTTACAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220758 representing NM_175571
  Red=Cloning site Green=Tags(s)
  
  MSEQSCQMSELRLLLLGKCRSGKSATGNAILGKHVFKSKFSDQTVIKMCQRESWVLRERKVVVIDTPDLF
  SSIACAEDKQRNIQHCLELSAPSLHALLLVIAIGHFTREDEETAKGIQQVFGAEARRHIIIVFTRKDDLG
  DDLLQDFIEKNKPLKQLVQDYEGRYCIFNNKTNSKDEQITQVLELLRKVESLVNTNGGPYHVNFKTEGSR
  FQDCVNEAASQEGDKPQGPRERQLQSTGPEQNPGTSELTVLLVGKRGAGKSAAGNSILGRQAFQTGFSEQ
  SVTQSFLSESRSWRKKKVSIIDAPDISSLKNIDSEVRKHICTGPHAFLLVTPLGFYTKNDEAVLSTIQNN
  FGEKFFEYMIILLTRKEDLGDQDLDTFLRNSNKALYGLIQKCKNRYSAFNYRATGEEEQRQADELLEKIE
  SMVHQNGNKHCVFREKETLNIVLVGRSGTGKSATGNSILGSLVFTSRLRAQPVTKTSQSGRRTWDGQEVV
  VVDTPSFNQMLDVEKDPSRLEEEVKRCLSCCEKGDTFFVLVFQLGRFTEEDKTAVAKLEAIFGADFTKYA
  IMLFTRKEDLGAGNLEDFMKNSDNKALRRIFKKCGRRVCAFNNKETGQAQETQVKALLTKVNDLRKESGW
  SGYPHTQENVSKLIKNVQEMSQAEKLLKNLIGILQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175571
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175571.4
• RefSeq Size: 3952 bp
• RefSeq ORF: 1998 bp
• Locus ID: 155038
• UniProt ID: Q8ND71
• Cytogenetics: 7q36.1
• Gene Summary: This gene encodes a protein belonging to the GTP-binding superfamily and to the immuno-associated nucleotide (IAN) subfamily of nucleotide-binding proteins. In humans, the IAN subfamily genes are located in a cluster at 7q36.1. [provided by RefSeq, Jul 2008]