DNA Ligase IV (LIG4) (NM_206937) Human Tagged ORF Clone

CAT#: RG220071

LIG4 (tGFP-tagged) - Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 2

Product Images

Circular map for RG220071

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DNA Ligase IV
• Synonyms: LIG4S
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220071 representing NM_206937
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCTCACAAACTTCACAAACTGTTGCATCTCACGTTCCTTTTGCAGATTTGTGTTCAACTTTAG
  AACGAATACAGAAAAGTAAAGGACGTGCAGAAAAAATCAGACACTTCAGGGAATTTTTAGATTCTTGGAG
  AAAATTTCATGATGCTCTTCATAAGAACCACAAAGATGTCACAGACTCTTTTTATCCAGCAATGAGACTA
  ATTCTTCCTCAGCTAGAAAGAGAGAGAATGGCCTATGGAATTAAAGAAACTATGCTTGCTAAGCTTTATA
  TTGAGTTGCTTAATTTACCTAGAGATGGAAAAGATGCCCTCAAACTTTTAAACTACAGAACACCCACTGG
  AACTCATGGAGATGCTGGAGACTTTGCAATGATTGCATATTTTGTGTTGAAGCCAAGATGTTTACAGAAA
  GGAAGTTTAACCATACAGCAAGTAAACGACCTTTTAGACTCAATTGCCAGCAATAATTCTGCTAAAAGAA
  AAGACCTAATAAAAAAGAGCCTTCTTCAACTTATAACTCAGAGTTCAGCACTTGAGCAAAAGTGGCTTAT
  ACGGATGATCATAAAGGATTTAAAGCTTGGTGTTAGTCAGCAAACTATCTTTTCTGTTTTTCATAATGAT
  GCTGCTGAGTTGCATAATGTCACTACAGATCTGGAAAAAGTCTGTAGGCAACTGCATGATCCTTCTGTAG
  GACTCAGTGATATTTCTATCACTTTATTTTCTGCATTTAAACCAATGCTAGCTGCTATTGCAGATATTGA
  GCACATTGAGAAGGATATGAAACATCAGAGTTTCTACATAGAAACCAAGCTAGATGGTGAACGTATGCAA
  ATGCACAAAGATGGAGATGTATATAAATACTTCTCTCGAAATGGATATAACTACACTGATCAGTTTGGTG
  CTTCTCCTACTGAAGGTTCTCTTACCCCATTCATTCATAATGCATTCAAAGCAGATATACAAATCTGTAT
  TCTTGATGGTGAGATGATGGCCTATAATCCTAATACACAAACTTTCATGCAAAAGGGAACTAAGTTTGAT
  ATTAAAAGAATGGTAGAGGATTCTGATCTGCAAACTTGTTATTGTGTTTTTGATGTATTGATGGTTAATA
  ATAAAAAGCTAGGGCATGAGACTCTGAGAAAGAGGTATGAGATTCTTAGTAGTATTTTTACACCAATTCC
  AGGTAGAATAGAAATAGTGCAGAAAACACAAGCTCATACTAAGAATGAAGTAATTGATGCATTGAATGAA
  GCAATAGATAAAAGAGAAGAGGGAATTATGGTAAAACAACCTCTATCCATCTACAAGCCAGACAAAAGAG
  GTGAAGGGTGGTTAAAAATTAAACCAGAGTATGTCAGTGGACTAATGGATGAATTGGACATTTTAATTGT
  TGGAGGATATTGGGGTAAAGGATCACGGGGTGGAATGATGTCTCATTTTCTGTGTGCAGTAGCAGAGAAG
  CCCCCTCCTGGTGAGAAGCCATCTGTGTTTCATACTCTCTCTCGTGTTGGGTCTGGCTGCACCATGAAAG
  AACTGTATGATCTGGGTTTGAAATTGGCCAAGTATTGGAAGCCTTTTCATAGAAAAGCTCCACCAAGCAG
  CATTTTATGTGGAACAGAGAAGCCAGAAGTATACATTGAACCTTGTAATTCTGTCATTGTTCAGATTAAA
  GCAGCAGAGATCGTACCCAGTGATATGTATAAAACTGGCTGCACCTTGCGTTTTCCACGAATTGAAAAGA
  TAAGAGATGACAAGGAGTGGCATGAGTGCATGACCCTGGACGACCTAGAACAACTTAGGGGGAAGGCATC
  TGGTAAGCTCGCATCTAAACACCTTTATATAGGTGGTGATGATGAACCACAAGAAAAAAAGCGGAAAGCT
  GCCCCAAAGATGAAGAAAGTTATTGGAATTATTGAGCACTTAAAAGCACCTAACCTTACTAACGTTAACA
  AAATTTCTAATATATTTGAAGATGTAGAGTTTTGTGTTATGAGTGGAACAGATAGCCAGCCAAAGCCTGA
  CCTGGAGAACAGAATTGCAGAATTTGGTGGTTATATAGTACAAAATCCAGGCCCAGACACGTACTGTGTA
  ATTGCAGGGTCTGAGAACATCAGAGTGAAAAACATAATTTTGTCAAATAAACATGATGTTGTCAAGCCTG
  CATGGCTTTTAGAATGTTTTAAGACCAAAAGCTTTGTACCATGGCAGCCTCGCTTTATGATTCATATGTG
  CCCATCAACCAAAGAACATTTTGCCCGTGAATATGATTGCTATGGTGATAGTTATTTCATTGATACAGAC
  TTGAACCAACTGAAGGAAGTATTCTCAGGAATTAAAAATTCTAACGAGCAGACTCCTGAAGAAATGGCTT
  CTCTGATTGCTGATTTAGAATATCGGTATTCCTGGGATTGCTCTCCTCTCAGTATGTTTCGACGCCACAC
  CGTTTATTTGGACTCGTATGCTGTTATTAATGACCTGAGTACCAAAAATGAGGGGACAAGGTTAGCTATT
  AAAGCCTTGGAGCTTCGGTTTCATGGAGCAAAAGTAGTTTCTTGTTTAGCTGAGGGAGTGTCTCATGTAA
  TAATTGGGGAAGATCATAGTCGTGTTGCAGATTTTAAAGCTTTTAGAAGAACTTTTAAGAGAAAGTTTAA
  AATCCTAAAAGAAAGTTGGGTAACTGATTCAATAGACAAGTGTGAATTACAAGAAGAAAACCAGTATTTG
  ATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220071 representing NM_206937
  Red=Cloning site Green=Tags(s)
  
  MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRL
  ILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQK
  GSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHND
  AAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQ
  MHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFD
  IKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNE
  AIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEK
  PPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIK
  AAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKA
  APKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCV
  IAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTD
  LNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAI
  KALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYL
  I
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206937
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206937.2
• RefSeq Size: 3994 bp
• RefSeq ORF: 2736 bp
• Locus ID: 3981
• UniProt ID: P49917
• Cytogenetics: 13q33.3
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• Gene Summary: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]