Sterol carrier protein 2 (SCP2) (NM_002979) Human Tagged ORF Clone

CAT#: RG219802

SCP2 (tGFP-tagged) - Human sterol carrier protein 2 (SCP2), transcript variant 1

Product Images

Circular map for RG219802

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sterol carrier protein 2
• Synonyms: NLTP; NSL-TP; SCOX; SCP-2; SCP-CHI; SCP-X; SCPX
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219802 representing NM_002979
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCTTCCCCGTGGGAGCCTGCGACCCTGCGCCGGGTGTTCGTGGTGGGGGTTGGCATGACCAAGT
  TTGTGAAGCCTGGAGCTGAGAATTCAAGAGACTACCCTGACTTGGCAGAAGAAGCAGGCAAGAAGGCTTT
  AGCTGATGCACAGATCCCTTATTCAGCAGTGGACCAGGCATGTGTTGGCTATGTTTTTGGTGACTCTACC
  TGTGGGCAGAGGGCTATCTATCACAGTTTGGGAATGACTGGAATTCCTATAATCAATGTCAACAATAACT
  GTGCTACTGGTTCTACTGCTTTGTTTATGGCCCGCCAGCTGATTCAGGGTGGTGTGGCAGAATGTGTCTT
  GGCTCTTGGGTTTGAGAAGATGAGTAAGGGAAGCCTTGGAATAAAATTTTCAGATAGAACCATTCCCACT
  GATAAGCATGTTGACCTCCTGATCAATAAGTATGGATTGTCTGCTCACCCAGTTGCTCCTCAGATGTTTG
  GGTATGCTGGAAAAGAACATATGGAAAAATATGGAACAAAAATTGAACACTTTGCAAAAATTGGATGGAA
  AAATCATAAACATTCAGTTAATAACCCGTATTCCCAGTTCCAAGATGAATACAGTTTAGATGAAGTGATG
  GCATCTAAAGAAGTTTTTGATTTTTTGACTATCTTACAATGTTGTCCCACTTCAGATGGTGCTGCAGCAG
  CAATTTTGGCCAGTGAAGCATTTGTACAGAAGTATGGCCTGCAATCCAAAGCTGTGGAAATTTTGGCACA
  AGAAATGATGACTGATTTGCCAAGCTCGTTTGAAGAAAAAAGCATTATTAAAATGGTTGGCTTTGATATG
  AGTAAAGAAGCTGCAAGAAAATGCTATGAGAAATCTGGCCTGACACCAAATGATATTGACGTAATAGAAC
  TTCACGATTGCTTTTCTACCAACGAACTCCTTACTTATGAAGCACTGGGACTCTGTCCAGAAGGACAAGG
  TGCAACGCTGGTTGATAGAGGAGATAATACATATGGAGGAAAGTGGGTCATAAATCCTAGTGGTGGACTG
  ATTTCAAAGGGACACCCACTAGGCGCTACAGGTCTTGCTCAGTGTGCAGAACTCTGCTGGCAGCTGAGAG
  GGGAAGCCGGAAAGAGGCAAGTTCCTGGTGCAAAGGTGGCTCTGCAGCATAATTTAGGCATTGGAGGAGC
  TGTGGTTGTAACACTCTACAAGATGGGTTTTCCGGAAGCCGCCAGTTCTTTTAGAACTCATCAAATTGAA
  GCTGTTCCAACCAGCTCTGCAAGTGATGGATTTAAGGCAAATCTTGTTTTTAAGGAGATTGAGAAGAAAC
  TTGAAGAGGAAGGGGAACAGTTTGTGAAGAAAATCGGTGGTATTTTTGCCTTCAAGGTGAAAGATGGCCC
  TGGGGGTAAAGAGGCCACCTGGGTGGTGGATGTGAAGAATGGCAAAGGATCAGTGCTTCCTAACTCAGAT
  AAGAAGGCTGACTGCACAATCACAATGGCTGACTCAGACTTCCTGGCTTTAATGACTGGTAAAATGAATC
  CTCAGTCGGCCTTCTTTCAAGGCAAATTGAAAATCACTGGCAACATGGGTCTCGCTATGAAGTTACAAAA
  TCTTCAGCTTCAGCCAGGCAACGCTAAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219802 representing NM_002979
  Red=Cloning site Green=Tags(s)
  
  MSSSPWEPATLRRVFVVGVGMTKFVKPGAENSRDYPDLAEEAGKKALADAQIPYSAVDQACVGYVFGDST
  CGQRAIYHSLGMTGIPIINVNNNCATGSTALFMARQLIQGGVAECVLALGFEKMSKGSLGIKFSDRTIPT
  DKHVDLLINKYGLSAHPVAPQMFGYAGKEHMEKYGTKIEHFAKIGWKNHKHSVNNPYSQFQDEYSLDEVM
  ASKEVFDFLTILQCCPTSDGAAAAILASEAFVQKYGLQSKAVEILAQEMMTDLPSSFEEKSIIKMVGFDM
  SKEAARKCYEKSGLTPNDIDVIELHDCFSTNELLTYEALGLCPEGQGATLVDRGDNTYGGKWVINPSGGL
  ISKGHPLGATGLAQCAELCWQLRGEAGKRQVPGAKVALQHNLGIGGAVVVTLYKMGFPEAASSFRTHQIE
  AVPTSSASDGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSVLPNSD
  KKADCTITMADSDFLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNLQLQPGNAKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002979
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002979.5
• RefSeq Size: 2697 bp
• RefSeq ORF: 1644 bp
• Locus ID: 6342
• UniProt ID: P22307
• Cytogenetics: 1p32.3
• Domains: thiolase, SCP2
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
• Gene Summary: This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.[provided by RefSeq, Aug 2010]