Dopamine Transporter (SLC6A3) (NM_001044) Human Tagged ORF Clone

CAT#: RG219466

SLC6A3 (tGFP-tagged) - Human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3)

Product Images

Circular map for RG219466

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dopamine Transporter
• Synonyms: DAT; DAT1; PKDYS; PKDYS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219466 representing NM_001044
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGAGCAAATGCTCCGTGGGACTCATGTCTTCCGTGGTGGCCCCGGCTAAGGAGCCCAATGCCG
  TGGGCCCGAAGGAGGTGGAGCTCATCCTTGTCAAGGAGCAGAACGGAGTGCAGCTCACCAGCTCCACCCT
  CACCAACCCGCGGCAGAGCCCCGTGGAGGCCCAGGATCGGGAGACCTGGGGCAAGAAGATCGACTTTCTC
  CTGTCCGTCATTGGCTTTGCTGTGGACCTGGCCAACGTCTGGCGGTTCCCCTACCTGTGCTACAAAAATG
  GTGGCGGTGCCTTCCTGGTCCCCTACCTGCTCTTCATGGTCATTGCTGGGATGCCACTTTTCTACATGGA
  GCTGGCCCTCGGCCAGTTCAACAGGGAAGGGGCCGCTGGTGTCTGGAAGATCTGCCCCATACTGAAAGGT
  GTGGGCTTCACGGTCATCCTCATCTCACTGTATGTCGGCTTCTTCTACAACGTCATCATCGCCTGGGCGC
  TGCACTATCTCTTCTCCTCCTTCACCACGGAGCTCCCCTGGATCCACTGCAACAACTCCTGGAACAGCCC
  CAACTGCTCGGATGCCCATCCTGGTGACTCCAGTGGAGACAGCTCGGGCCTCAACGACACTTTTGGGACC
  ACACCTGCTGCCGAGTACTTTGAACGTGGCGTGCTGCACCTCCACCAGAGCCATGGCATCGACGACCTGG
  GGCCTCCGCGGTGGCAGCTCACAGCCTGCCTGGTGCTGGTCATCGTGCTGCTCTACTTCAGCCTCTGGAA
  GGGCGTGAAGACCTCAGGGAAGGTGGTATGGATCACAGCCACCATGCCATACGTGGTCCTCACTGCCCTG
  CTCCTGCGTGGGGTCACCCTCCCTGGAGCCATAGACGGCATCAGAGCATACCTGAGCGTTGACTTCTACC
  GGCTCTGCGAGGCGTCTGTTTGGATTGACGCGGCCACCCAGGTGTGCTTCTCCCTGGGCGTGGGGTTCGG
  GGTGCTGATCGCCTTCTCCAGCTACAACAAGTTCACCAACAACTGCTACAGGGACGCGATTGTCACCACC
  TCCATCAACTCCCTGACGAGCTTCTCCTCCGGCTTCGTCGTCTTCTCCTTCCTGGGGTACATGGCACAGA
  AGCACAGTGTGCCCATCGGGGACGTGGCCAAGGACGGGCCAGGGCTGATCTTCATCATCTACCCGGAAGC
  CATCGCCACGCTCCCTCTGTCCTCAGCCTGGGCCGTGGTCTTCTTCATCATGCTGCTCACCCTGGGTATC
  GACAGCGCCATGGGTGGTATGGAGTCAGTGATCACCGGGCTCATCGATGAGTTCCAGCTGCTGCACAGAC
  ACCGTGAGCTCTTCACGCTCTTCATCGTCCTGGCGACCTTCCTCCTGTCCCTGTTCTGCGTCACCAACGG
  TGGCATCTACGTCTTCACGCTCCTGGACCATTTTGCAGCCGGCACGTCCATCCTCTTTGGAGTGCTCATC
  GAAGCCATCGGAGTGGCCTGGTTCTATGGTGTTGGGCAGTTCAGCGACGACATCCAGCAGATGACCGGGC
  AGCGGCCCAGCCTGTACTGGCGGCTGTGCTGGAAGCTGGTCAGCCCCTGCTTTCTCCTGTTCGTGGTCGT
  GGTCAGCATTGTGACCTTCAGACCCCCCCACTACGGAGCCTACATCTTCCCCGACTGGGCCAACGCGCTG
  GGCTGGGTCATCGCCACATCCTCCATGGCCATGGTGCCCATCTATGCGGCCTACAAGTTCTGCAGCCTGC
  CTGGGTCCTTTCGAGAGAAACTGGCCTACGCCATTGCACCCGAGAAGGACCGTGAGCTGGTGGACAGAGG
  GGAGGTGCGCCAGTTCACGCTCCGCCACTGGCTCAAGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219466 representing NM_001044
  Red=Cloning site Green=Tags(s)
  
  MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRETWGKKIDFL
  LSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPILKG
  VGFTVILISLYVGFFYNVIIAWALHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDTFGT
  TPAAEYFERGVLHLHQSHGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTAL
  LLRGVTLPGAIDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIVTT
  SINSLTSFSSGFVVFSFLGYMAQKHSVPIGDVAKDGPGLIFIIYPEAIATLPLSSAWAVVFFIMLLTLGI
  DSAMGGMESVITGLIDEFQLLHRHRELFTLFIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLI
  EAIGVAWFYGVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANAL
  GWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001044
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001044.5
• RefSeq Size: 3945 bp
• RefSeq ORF: 1863 bp
• Locus ID: 6531
• UniProt ID: Q01959
• Cytogenetics: 5p15.33
• Domains: SNF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Parkinson's disease
• Gene Summary: This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]