CMTM1 (NM_181268) Human Tagged ORF Clone

CAT#: RG219038

CMTM1 (tGFP-tagged) - Human CKLF-like MARVEL transmembrane domain containing 1 (CMTM1), transcript variant 18

Product Images

Circular map for RG219038

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CMTM1
• Synonyms: CKLFH; CKLFH1; CKLFSF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219038 representing NM_181268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCTGAACACGCCAAACCTGAGTCATCCGAGGCACCTTCAGGGAACTTGAAACAACCGGAGACTG
  CCGCAGCCCTGGCAAGTAGCGGCAGCGTAGTGAGTTCTGTACCCAAGGCACAGCGCAACATCTCAGCGAA
  GACCGCACCCCGGAAGCACCCCGCAGTCTCAATTCGCAGTGCGCAGTCCGCAGCCGCCGCACGTCCCCAA
  GGCAGTGAGGGCACCGCACCCTCAAGGAAAGCCACCACACGCCCACCCCCAAAGCCCACACTCCCACCCC
  CCACGCCCTCTGCACACACTGAATCCAAACTCTTAAATGAGATGGCGATCAAAGAGCGCGTGGAGGGCCG
  AGCCAAAGTCCCGTACAAATTCAGGGACAGCCTCAAACGTTTCTCCTTCTCGCCCACTGGAATGTTGAAG
  ATCCTGAGACTGAGTCTTATCTTAGGAGCATTAGCTTGTTTCATCATCACCCAAGCCAATGAGTCATTTA
  TAACAATCACAAGTCTGGAAATCTGCATTGTCGTTTTTTTTATTCTAATATATGTGCTAACCCTTCACCA
  CTTGCTGACCTATTTACATTGGCCCTTACTTGATCTTACCAACAGTATCATTACAGCTGTGTTCCTTTCA
  GTAGTTGCCATCTTGGCCATGCAAGAAAAGAAAAGAAGGCATTTACTCTATGTCGGGGGGCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219038 representing NM_181268
  Red=Cloning site Green=Tags(s)
  
  MDPEHAKPESSEAPSGNLKQPETAAALASSGSVVSSVPKAQRNISAKTAPRKHPAVSIRSAQSAAAARPQ
  GSEGTAPSRKATTRPPPKPTLPPPTPSAHTESKLLNEMAIKERVEGRAKVPYKFRDSLKRFSFSPTGMLK
  ILRLSLILGALACFIITQANESFITITSLEICIVVFFILIYVLTLHHLLTYLHWPLLDLTNSIITAVFLS
  VVAILAMQEKKRRHLLYVGGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181268
• ORF Size: 693 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181268.3
• RefSeq Size: 1065 bp
• RefSeq ORF: 696 bp
• Locus ID: 113540
• UniProt ID: Q8IZ96
• Cytogenetics: 16q21
• Protein Families: Transmembrane
• Gene Summary: This gene belongs to the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies of signaling molecules. The protein encoded by this gene may play an important role in testicular development. Alternatively spliced transcript variants encoding different isoforms have been identified. Naturally occurring read-through transcription occurs between this locus and the neighboring locus CKLF (chemokine-like factor).[provided by RefSeq, Feb 2011]