PMP70 (ABCD3) (NM_002858) Human Tagged ORF Clone

CAT#: RG217812

ABCD3 (tGFP-tagged) - Human ATP-binding cassette, sub-family D (ALD), member 3 (ABCD3), transcript variant 1

Product Images

Circular map for RG217812

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PMP70
• Synonyms: ABC43; CBAS5; PMP70; PXMP1; ZWS2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217812 representing NM_002858
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTTCAGCAAGTACTTGACGGCGCGGAACTCCTCGCTGGCTGGTGCCGCGTTCCTGCTGCTCT
  GCCTGCTCCACAAGCGGCGCCGCGCCCTCGGCCTGCACGGTAAGAAAAGTGGAAAACCACCATTACAGAA
  CAATGAGAAAGAGGGGAAAAAAGAGCGAGCTGTGGTGGACAAGGTGTTTTTCTCAAGGCTCATACAGATT
  CTGAAAATCATGGTCCCTAGAACATTTTGTAAAGAGACAGGTTACTTGGTACTTATTGCTGTTATGCTGG
  TGTCTCGAACATATTGTGATGTTTGGATGATTCAAAATGGGACACTAATTGAAAGTGGTATCATTGGTCG
  TAGCAGGAAAGATTTCAAGAGATACTTACTCAACTTCATCGCTGCCATGCCTCTTATCTCTCTGGTTAAT
  AACTTCTTGAAGTATGGGTTAAATGAGCTTAAACTGTGCTTCCGAGTAAGGCTCACTAAATACCTCTATG
  AGGAGTATCTTCAAGCTTTCACATATTATAAAATGGGGAATCTGGACAACAGAATAGCTAATCCAGACCA
  GCTGCTTACACAAGATGTAGAAAAATTTTGTAACAGTGTAGTCGATCTGTATTCAAATCTTAGTAAGCCA
  TTTTTAGACATAGTTTTGTATATCTTTAAGTTAACGAGTGCAATTGGAGCTCAGGGCCCAGCGAGCATGA
  TGGCCTACTTGGTTGTTTCTGGGCTATTCCTAACTCGACTTCGAAGACCCATTGGTAAGATGACAATAAC
  TGAGCAAAAGTATGAAGGAGAATATAGATATGTTAATTCTCGGCTCATCACAAACAGTGAAGAAATTGCC
  TTTTACAATGGGAATAAAAGAGAAAAGCAGACAGTCCACTCAGTCTTCCGAAAACTGGTGGAACACCTAC
  ATAATTTCATTTTGTTTCGGTTTTCAATGGGCTTCATTGATAGTATTATTGCCAAATACCTTGCCACTGT
  TGTTGGTTACCTAGTTGTCAGTCGCCCTTTCTTAGATTTGTCTCATCCTCGACATCTCAAGAGTACACAT
  TCGGAACTTCTAGAGGATTACTACCAAAGTGGAAGAATGCTTTTGCGAATGTCTCAAGCTCTGGGTCGAA
  TAGTTTTGGCTGGGCGTGAAATGACTAGATTGGCCGGTTTTACTGCTCGGATTACAGAATTAATGCAAGT
  ACTGAAGGATTTAAATCATGGCAAATATGAGCGCACAATGGTCTCACAACAGGAAAAGGGTATTGAAGGA
  GTACAAGTCATTCCCTTGATACCTGGTGCTGGAGAAATCATTATTGCAGATAACATTATAAAGTTTGATC
  ATGTTCCTTTAGCAACGCCAAATGGAGATGTTTTGATCCGAGACCTTAATTTTGAAGTTCGATCTGGGGC
  TAATGTTCTAATTTGTGGTCCAAATGGCTGCGGAAAGAGTTCACTTTTCCGTGTTCTTGGTGAATTATGG
  CCTCTTTTTGGAGGACGTCTAACTAAACCTGAAAGAGGAAAATTATTTTATGTTCCTCAGAGACCTTACA
  TGACCCTTGGAACACTTCGAGATCAAGTGATATATCCAGATGGACGAGAAGATCAGAAAAGGAAGGGAAT
  TTCTGACCTAGTACTGAAGGAATACTTAGACAATGTCCAGTTGGGTCATATCCTTGAACGTGAAGGAGGC
  TGGGACAGTGTTCAGGATTGGATGGACGTACTCAGTGGTGGAGAAAAGCAAAGAATGGCGATGGCAAGAT
  TATTTTATCATAAACCCCAGTTTGCCATTTTGGATGAATGCACAAGTGCAGTTAGTGTCGACGTGGAAGG
  CTACATTTATAGTCATTGTCGAAAGGTTGGCATCACTCTCTTCACTGTGTCTCATAGGAAATCTCTTTGG
  AAACATCATGAGTACTACCTGCATATGGATGGCAGAGGCAACTATGAATTCAAACAGATAACAGAAGATA
  CAGTTGAGTTTGGCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217812 representing NM_002858
  Red=Cloning site Green=Tags(s)
  
  MAAFSKYLTARNSSLAGAAFLLLCLLHKRRRALGLHGKKSGKPPLQNNEKEGKKERAVVDKVFFSRLIQI
  LKIMVPRTFCKETGYLVLIAVMLVSRTYCDVWMIQNGTLIESGIIGRSRKDFKRYLLNFIAAMPLISLVN
  NFLKYGLNELKLCFRVRLTKYLYEEYLQAFTYYKMGNLDNRIANPDQLLTQDVEKFCNSVVDLYSNLSKP
  FLDIVLYIFKLTSAIGAQGPASMMAYLVVSGLFLTRLRRPIGKMTITEQKYEGEYRYVNSRLITNSEEIA
  FYNGNKREKQTVHSVFRKLVEHLHNFILFRFSMGFIDSIIAKYLATVVGYLVVSRPFLDLSHPRHLKSTH
  SELLEDYYQSGRMLLRMSQALGRIVLAGREMTRLAGFTARITELMQVLKDLNHGKYERTMVSQQEKGIEG
  VQVIPLIPGAGEIIIADNIIKFDHVPLATPNGDVLIRDLNFEVRSGANVLICGPNGCGKSSLFRVLGELW
  PLFGGRLTKPERGKLFYVPQRPYMTLGTLRDQVIYPDGREDQKRKGISDLVLKEYLDNVQLGHILEREGG
  WDSVQDWMDVLSGGEKQRMAMARLFYHKPQFAILDECTSAVSVDVEGYIYSHCRKVGITLFTVSHRKSLW
  KHHEYYLHMDGRGNYEFKQITEDTVEFGS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002858
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002858.2, NP_002849.1
• RefSeq Size: 3423 bp
• RefSeq ORF: 1980 bp
• Locus ID: 5825
• UniProt ID: P28288
• Cytogenetics: 1p21.3
• Domains: ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]