ADAM28 (NM_014265) Human Tagged ORF Clone

CAT#: RG217775

ADAM28 (tGFP-tagged) - Human ADAM metallopeptidase domain 28 (ADAM28), transcript variant 1

Product Images

Circular map for RG217775

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADAM28
• Synonyms: ADAM 28; eMDC II; eMDCII; MDC-L; MDCL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217775 representing NM_014265
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCAAGGTCTCCTGCCAGTCAGTCTCCTCCTCTCTGTTGCAGTAAGTGCTATAAAAGAACTCCCTG
  GGGTGAAGAAGTATGAAGTGGTTTATCCTATAAGACTTCATCCACTGCATAAAAGAGAGGCCAAAGAGCC
  AGAGCAACAGGAACAATTTGAAACTGAATTAAAGTATAAAATGACAATTAATGGAAAAATTGCAGTGCTT
  TATTTGAAAAAAAACAAGAACCTCCTTGCACCAGGCTACACGGAAACATATTATAATTCCACTGGAAAGG
  AGATCACCACAAGCCCACAAATTATGGATGATTGTTATTATCAAGGACATATTCTTAATGAAAAGGTTTC
  TGACGCTAGCATCAGCACATGTAGGGGTCTAAGGGGCTACTTCAGTCAGGGGGATCAAAGATACTTTATT
  GAACCTTTAAGCCCCATACATCGGGATGGACAGGAGCATGCACTCTTCAAGTATAACCCTGATGAAAAGA
  ATTATGACAGCACCTGTGGGATGGATGGTGTGTTGTGGGCCCACGATTTGCAGCAGAACATTGCCCTACC
  TGCCACCAAACTAGTAAAATTGAAAGACAGGAAGGTTCAGGAACATGAGAAATACATAGAATATTATTTG
  GTCCTGGATAATGGTGAGTTTAAAAGGTACAATGAGAATCAAGATGAGATCAGAAAGAGGGTATTTGAGA
  TGGCTAATTATGTCAACATGCTTTATAAAAAGCTCAATACTCATGTGGCCTTAGTTGGTATGGAAATCTG
  GACTGACAAGGATAAGATAAAGATAACCCCAAATGCAAGCTTCACCTTGGAGAATTTTTCTAAATGGAGG
  GGGAGTGTTCTCTCAAGAAGAAAGCGTCATGATATTGCTCAGTTAATCACAGCAACAGAACTTGCTGGAA
  CGACTGTGGGTCTTGCATTTATGTCTACAATGTGTTCTCCTTATTCTGTTGGCGTTGTTCAGGACCACAG
  CGATAATCTTCTTAGAGTTGCAGGGACAATGGCACATGAAATGGGCCACAACTTTGGAATGTTTCATGAC
  GACTATTCTTGCAAGTGTCCTTCTACAATATGTGTGATGGACAAAGCACTGAGCTTCTATATACCCACAG
  ACTTCAGTTCCTGCAGCCGTCTCAGCTATGACAAGTTTTTTGAAGATAAATTATCAAATTGCCTCTTTAA
  TGCTCCATTGCCTACAGATATCATATCCACTCCAATTTGTGGGAACCAGTTGGTGGAAATGGGAGAGGAC
  TGTGATTGTGGGACATCTGAGGAATGTACCAATATTTGCTGTGATGCTAAGACATGTAAAATCAAAGCAA
  CTTTTCAATGTGCATTAGGAGAATGTTGTGAAAAATGCCAATTTAAAAAGGCTGGGATGGTGTGCAGACC
  AGCAAAAGATGAGTGCGACCTGCCTGAAATGTGTAATGGTAAATCTGGTAATTGTCCTGATGATAGATTC
  CAAGTCAATGGCTTCCCTTGCCATCACGGGAAGGGCCACTGCTTGATGGGGACATGCCCCACACTGCAGG
  AGCAGTGCACAGAGCTGTGGGGACCAGGAACTGAGGTTGCAGATAAGTCATGTTACAACAGGAATGAAGG
  TGGGTCAAAGTACGGGTACTGTCGCAGAGTGGATGACACACTCATTCCCTGCAAAGCAAATGATACCATG
  TGTGGGAAGTTGTTCTGTCAAGGTGGGTCGGATAATTTGCCCTGGAAAGGACGGATAGTGACTTTCCTGA
  CATGTAAAACATTTGATCCTGAAGACACAAGTCAAGAAATAGGCATGGTGGCCAATGGAACTAAGTGTGG
  CGATAACAAGGTTTGCATTAATGCAGAATGTGTGGATATTGAGAAAGCCTACAAATCAACCAATTGCTCA
  TCTAAGTGCAAAGGACATGCTGTGTGTGACCATGAGCTCCAGTGTCAATGTGAGGAAGGATGGATCCCTC
  CCGACTGCGATGACTCCTCAGTGGTCTTCCACTTCTCCATTGTGGTTGGGGTGCTGTTCCCAATGGCGGT
  CATTTTTGTGGTGGTTGCTATGGTAATCCGGCACCAGAGCTCCAGAGAAAAGCAGAAGAAAGATCAGAGG
  CCACTATCTACCACTGGCACCAGGCCACACAAACAGAAGAGGAAACCCCAGATGGTAAAGGCTGTTCAAC
  CCCAAGAGATGAGTCAGATGAAGCCCCATGTGTATGATCTGCCAGTAGAAGGCAATGAGCCCCCAGCCTC
  TTTTCATAAAGACACAAACGCACTTCCCCCTACTGTTTTCAAGGATAATCCAATGTCTACACCTAAGGAC
  TCAAATCCAAAAGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217775 representing NM_014265
  Red=Cloning site Green=Tags(s)
  
  MLQGLLPVSLLLSVAVSAIKELPGVKKYEVVYPIRLHPLHKREAKEPEQQEQFETELKYKMTINGKIAVL
  YLKKNKNLLAPGYTETYYNSTGKEITTSPQIMDDCYYQGHILNEKVSDASISTCRGLRGYFSQGDQRYFI
  EPLSPIHRDGQEHALFKYNPDEKNYDSTCGMDGVLWAHDLQQNIALPATKLVKLKDRKVQEHEKYIEYYL
  VLDNGEFKRYNENQDEIRKRVFEMANYVNMLYKKLNTHVALVGMEIWTDKDKIKITPNASFTLENFSKWR
  GSVLSRRKRHDIAQLITATELAGTTVGLAFMSTMCSPYSVGVVQDHSDNLLRVAGTMAHEMGHNFGMFHD
  DYSCKCPSTICVMDKALSFYIPTDFSSCSRLSYDKFFEDKLSNCLFNAPLPTDIISTPICGNQLVEMGED
  CDCGTSEECTNICCDAKTCKIKATFQCALGECCEKCQFKKAGMVCRPAKDECDLPEMCNGKSGNCPDDRF
  QVNGFPCHHGKGHCLMGTCPTLQEQCTELWGPGTEVADKSCYNRNEGGSKYGYCRRVDDTLIPCKANDTM
  CGKLFCQGGSDNLPWKGRIVTFLTCKTFDPEDTSQEIGMVANGTKCGDNKVCINAECVDIEKAYKSTNCS
  SKCKGHAVCDHELQCQCEEGWIPPDCDDSSVVFHFSIVVGVLFPMAVIFVVVAMVIRHQSSREKQKKDQR
  PLSTTGTRPHKQKRKPQMVKAVQPQEMSQMKPHVYDLPVEGNEPPASFHKDTNALPPTVFKDNPMSTPKD
  SNPKA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014265
• ORF Size: 2325 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014265.3, NP_055080.1
• RefSeq Size: 3220 bp
• RefSeq ORF: 2328 bp
• Locus ID: 10863
• UniProt ID: Q9UKQ2
• Cytogenetics: 8p21.2
• Domains: Reprolysin, DISIN, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]