DGKD (NM_152879) Human Tagged ORF Clone

CAT#: RG217053

DGKD (tGFP-tagged) - Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 2

Product Images

Circular map for RG217053

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DGKD
• Synonyms: DGK-delta; dgkd-2; DGKdelta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217053 representing NM_152879
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGGCGCCCCTCCGCCGGGTCCCCCGCAACCGCCTCCGCCGCCGCCGCCCGAGGAGT
  CGTCCGACAGCGAGCCCGAGGCGGAGCCCGGCTCCCCACAGAAGCTCATCCGCAAGGTGTCCACGTCGGG
  TCAGATCCGACAGAAGACCATCATCAAAGAGGGGATGCTGACCAAACAGAACAATTCATTCCAGCGATCA
  AAAAGGAGATACTTTAAGCTTCGAGGGCGAACGCTTTACTATGCCAAAACGGCAAAGTCAATCATATTTG
  ATGAGGTGGATCTGACAGATGCCAGCGTAGCTGAATCCAGTACCAAAAACGTCAACAACAGTTTTACGGT
  CATAACTCCATGCAGGAAGCTCATCTTGTGTGCTGATAACAGAAAAGAAATGGAAGATTGGATTGCAGCA
  TTAAAGACTGTGCAGAACAGGGAGCACTTTGAGCCCACCCAGTACAGCATGGACCACTTCTCAGGGATGC
  ACAATTGGTACGCCTGTTCCCACGCGAGGCCGACCTACTGCAATGTGTGCCGTGAGGCTCTGTCTGGGGT
  CACGTCGCACGGGCTGTCCTGCGAGGTGTGCAAATTTAAGGCCCACAAGCGCTGTGCTGTGCGTGCAACC
  AATAACTGCAAGTGGACCACACTGGCCTCGATCGGGAAGGACATCATTGAAGATGCAGATGGGATTGCAA
  TGCCCCACCAGTGGTTGGAAGGAAACCTACCTGTGAGCGCCAAGTGCACTGTGTGCGACAAGACCTGTGG
  CAGTGTGCTGCGCCTGCAGGACTGGCGCTGCCTCTGGTGCAAGGCCATGGTTCACACATCGTGTAAAGAA
  TCCTTGCTGACCAAGTGCCCACTTGGCCTGTGCAAAGTGTCAGTCATCCCACCCACGGCTCTCAACAGCA
  TCGACTCCGATGGGTTCTGGAAGGCCAGCTGTCCTCCTTCTTGCACAAGCCCACTGTTGGTCTTCGTCAA
  TTCAAAAAGTGGGGACAACCAGGGTGTGAAGTTCCTCAGAAGATTCAAACAGCTACTAAACCCCGCCCAG
  GTCTTCGACCTCATGAACGGAGGCCCACACCTCGGCTTACGGTTATTCCAGAAGTTTGACACATTCCGGA
  TTCTGGTTTGTGGCGGGGATGGAAGTGTTGGCTGGGTCCTCTCCGAAATCGACAGCCTCAACCTTCATAA
  ACAGTGTCAGCTGGGAGTGCTGCCGCTCGGCACAGGGAACGACTTGGCCCGAGTACTGGGCTGGGGCTCA
  GCCTGCGATGACGACACCCAGCTCCCCCAGATCTTGGAGAAGTTGGAGAGAGCCAGCACCAAGATGCTGG
  ACAGGTGGAGCGTCATGGCATACGAGGCCAAGCTCCCCCGGCAGGCCTCCTCCTCTACCGTCACCGAAGA
  CTTCAGCGAGGATTCCGAGGTACAGCAGATTCTCTTCTATGAAGACTCGGTTGCAGCCCACCTTTCTAAA
  ATCCTCACCTCGGACCAGCACTCGGTGGTCATCTCCTCGGCCAAAGTGCTCTGTGAGACGGTGAAGGACT
  TCGTGGCACGGGTGGGGAAGGCCTATGAGAAGACGACCGAGAGCTCGGAGGAGTCAGAGGTCATGGCCAA
  GAAGTGCTCTGTCCTGAAAGAGAAGCTGGATTCCCTTCTCAAGACCTTGGACGATGAGTCCCAGGCCTCG
  TCCTCTCTGCCCAACCCGCCCCCCACCATTGCCGAGGAGGCTGAAGATGGAGATGGGTCGGGCAGCATCT
  GCGGTTCCACCGGAGACCGCTTGGTGGCATCAGCTTGCCCGGCCCGGCCGCAGATATTCCGGCCTCGAGA
  ACAGCTCATGCTGAGAGCCAACAGCCTGAAGAAAGCAATTCGTCAGATCATAGAACACACAGAAAAAGCT
  GTCGATGAGCAGAATGCCCAGACCCAGGAGCAGGAGGGCTTCGTCCTGGGCCTCTCTGAGTCAGAGGAGA
  AGATGGACCACAGAGTGTGCCCACCACTGTCCCACAGCGAGAGCTTCGGGGTCCCCAAGGGGAGGAGCCA
  GCGCAAAGTGTCGAAATCTCCGTGTGAAAAGCTGATCAGCAAAGGGAGTCTGTCCCTAGGCAGTTCTGCT
  TCCCTTCCGCCCCAGCCGGGAAGCCGGGACGGCCTGCCTGCGCTCAACACCAAGATCCTGTACCCAAATG
  TCCGGGCTGGAATGTCTGGTTCCTTACCCGGTGGCTCAGTCATCAGTCGCCTGTTAATTAATGCTGATCC
  CTTCAACTCTGAACCAGAAACCCTAGAGTATTACACGGAGAAATGTGTCATGAACAACTATTTTGGCATT
  GGCCTGGATGCGAAGATATCCCTGGACTTTAACAACAAGCGCGATGAGCACCCAGAGAAGTGCAGGAGCC
  GAACCAAGAACATGATGTGGTATGGAGTTCTTGGAACCAAAGAGTTGCTGCACAGAACCTACAAGAACCT
  GGAGCAAAAGGTCTTGCTGGAGTGTGACGGGCGACCCATCCCACTCCCCAGTCTTCAGGGAATTGCTGTC
  CTTAACATTCCCAGCTATGCCGGAGGAACCAACTTCTGGGGGGGTACCAAGGAAGATGATACTTTCGCAG
  CTCCATCATTCGATGACAAGATTCTGGAGGTGGTCGCCGTGTTCGGCAGCATGCAGATGGCCGTCTCTCG
  AGTCATCAGGCTACAGCATCATCGGATCGCCCAGTGTCGCACGGTGAAGATCTCCATCCTTGGGGATGAG
  GGCGTGCCTGTGCAGGTGGACGGAGAGGCCTGGGTCCAGCCGCCAGGGTACATTCGGATTGTCCACAAGA
  ACCGGGCACAGACACTGACCAGAGACAGGGCATTTGAGAGCACCCTGAAGTCCTGGGAAGACAAGCAGAA
  GTGCGAGCTGCCCCGCCCTCCATCCTGTTCCCTGCACCCGGAGATGCTGTCCGAGGAGGAGGCCACCCAG
  ATGGACCAGTTTGGGCAGGCAGCAGGGGTCCTCATTCACAGTATCCGAGAAATAGCTCAGTCTCACCGGG
  ACATGGAGCAGGAACTGGCCCACGCCGTCAATGCCAGCTCCAAGTCCATGGACCGTGTGTATGGCAAGCC
  CAGAACCACAGAGGGGCTCAACTGCAGCTTCGTCCTGGAAATGGTGAATAACTTCAGAGCTCTGCGCAGT
  GAGACGGAGCTGCTGCTGTCTGGGAAGATGGCCCTGCAGCTGGATCCGCCTCAGAAGGAGCAGCTGGGGA
  GTGCTCTTGCCGAGATGGACCGACAGCTCAGGAGGCTGGCAGACACCCCGTGGCTCTGCCAGTCCGCAGA
  GCCCGGCGACGAAGAGAGTGTGATGCTGGATCTTGCCAAGCGCAGTCGCAGTGGTAAATTCCGCCTCGTG
  ACCAAGTTTAAAAAGGAGAAAAACAACAAGAACAAAGAAGCTCACAGTAGCCTGGGAGCCCCGGTTCACC
  TCTGGGGGACAGAGGAGGTTGCTGCCTGGCTGGAGCACCTCAGTCTCTGTGAGTATAAGGACATCTTCAC
  ACGGCACGACATCCGGGGCTCTGAGCTCCTGCACCTGGAGCGGAGGGACCTCAAGGACCTGGGCGTGACC
  AAGGTGGGCCACATGAAGAGGATCCTGTGTGGCATCAAGGAGCTGAGCCGCAGCGCCCCCGCCGTCGAGG
  CC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217053 representing NM_152879
  Red=Cloning site Green=Tags(s)
  
  MAAAAGAPPPGPPQPPPPPPPEESSDSEPEAEPGSPQKLIRKVSTSGQIRQKTIIKEGMLTKQNNSFQRS
  KRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAA
  LKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRAT
  NNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKE
  SLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQ
  VFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGS
  ACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSK
  ILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQAS
  SSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKA
  VDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSA
  SLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGI
  GLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAV
  LNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDE
  GVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQ
  MDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRS
  ETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLV
  TKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVT
  KVGHMKRILCGIKELSRSAPAVEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152879
• ORF Size: 3642 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152879.3
• RefSeq Size: 6294 bp
• RefSeq ORF: 3645 bp
• Locus ID: 8527
• UniProt ID: Q16760
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]