ANKLE2 (NM_015114) Human Tagged ORF Clone

CAT#: RG216867

ANKLE2 (tGFP-tagged) - Human ankyrin repeat and LEM domain containing 2 (ANKLE2)

Product Images

Circular map for RG216867

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANKLE2
• Synonyms: KIAA0692; Lem4; LEMD7; MCPH16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216867 representing NM_015114
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGCCGCGGCTGGCGGCGGCCGAGTGGGCGGCGCTGGCCTGGGAGCTGCTGGGCGCCTCGGTGC
  TGCTGATCGCTGTGCGGTGGCTGGTGCGGCGGCTGGGCCCGCGGCCGGGAGGTCTGGGCCGCAGCGGGAC
  CCCAGTTCCTCCGCCAAGCGCGGCCGCCGCCCCCGCCTCAGGTGAAATGACAATGGATGCTCTGTTGGCT
  CGATTGAAACTTCTGAATCCAGATGACCTTAGAGAAGAAATCGTCAAAGCCGGATTGAAATGTGGACCCA
  TTACATCAACTACAAGGTTCATTTTTGAGAAAAAATTGGCTCAGGCTTTACTGGAGCAAGGAGGAAGGCT
  GTCTTCTTTCTACCACCATGAGGCAGGTGTCACAGCTCTCAGCCAGGACCCACAAAGGATTTTGAAGCCA
  GCTGAAGGGAACCCAACTGATCAGGCTGGTTTTTCTGAAGACAGAGATTTTGGTTACAGTGTGGGCCTGA
  ATCCTCCAGAGGAGGAAGCTGTGACATCCAAGACCTGCTCGGTGCCCCCTAGTGACACCGACACCTACAG
  AGCTGGAGCGACTGCGTCTAAGGAGCCGCCCCTGTACTATGGGGTGTGTCCAGTGTATGAGGACGTCCCA
  GCGAGAAATGAAAGGATCTATGTTTATGAAAATAAAAAGGAAGCATTGCAAGCTGTCAAGATGATCAAAG
  GGTCCCGATTTAAAGCTTTTTCTACCAGAGAAGACGCTGAGAAATTTGCTAGAGGAATTTGTGATTATTT
  CCCTTCTCCAAGCAAAACGTCCTTACCACTGTCTCCTGTGAAAACAGCTCCACTCTTTAGCAATGACAGG
  TTGAAAGATGGTTTGTGCTTGTCGGAATCAGAAACAGTCAACAAAGAGCGAGCGAACAGTTACAAAAATC
  CCCGCACGCAGGACCTCACCGCCAAGCTTCGGAAAGCTGTGGAGAAGGGAGAGGAGGACACCTTTTCTGA
  CCTTATCTGGAGCAACCCCCGGTATCTGATAGGCTCAGGAGACAACCCCACTATCGTGCAGGAAGGGTGC
  AGGTACAACGTGATGCATGTTGCTGCCAAAGAGAACCAGGCTTCCATCTGCCAGCTGACTCTGGACGTCC
  TGGAGAACCCTGACTTCATGAGGCTGATGTACCCTGATGACGACGAGGCCATGCTGCAGAAGCGTATCCG
  TTACGTGGTGGACCTGTACCTCAACACCCCCGACAAGATGGGCTATGACACACCGTTGCATTTTGCTTGT
  AAGTTTGGAAATGCAGATGTAGTCAACGTGCTTTCGTCACACCATTTGATTGTAAAAAACTCAAGGAATA
  AATATGATAAAACACCTGAAGATGTAATTTGTGAAAGAAGCAAAAATAAATCTGTGGAACTGAAGGAGCG
  GATCAGAGAGTATTTAAAGGGCCACTACTACGTGCCCCTCCTGAGAGCGGAAGAGACTTCTTCTCCAGTC
  ATCGGGGAGCTGTGGTCCCCAGACCAGACGGCTGAGGCCTCTCACGTCAGCCGCTATGGAGGCAGCCCCA
  GAGACCCGGTACTGACCCTGAGAGCCTTCGCAGGGCCCCTGAGTCCAGCCAAGGCAGAAGATTTTCGCAA
  GCTCTGGAAAACTCCACCTCGAGAGAAAGCAGGCTTCCTTCACCACGTCAAGAAGTCGGACCCGGAAAGA
  GGCTTTGAGAGAGTGGGAAGGGAGCTAGCTCATGAGCTGGGGTATCCCTGGGTTGAATACTGGGAATTTC
  TGGGCTGTTTTGTTGATCTGTCTTCCCAGGAAGGCCTGCAAAGACTAGAAGAATATCTCACACAGCAGGA
  AATAGGCAAAAAGGCTCAACAAGAAACAGGAGAACGGGAAGCCTCCTGCCGAGATAAAGCCACCACGTCT
  GGCAGCAATTCCATTTCCGTGAGGGCGTTTCTAGATGAAGATGACATGAGCTTGGAAGAAATAAAAAATC
  GGCAAAATGCAGCTCGAAATAACAGCCCGCCCACAGTCGGTGCTTTTGGACATACGAGGTGCAGCGCCTT
  CCCCTTGGAGCAGGAGGCAGACCTCATAGAAGCCGCCGAGCCGGGAGGTCCACACAGCAGCAGAAATGGG
  CTCTGCCATCCTCTGAATCACAGCAGGACCCTGGCGGGCAAGAGACCAAAGGCCCCCCGTGGGGAGGAAG
  CCCATCTGCCACCTGTCTCGGATTTGACTGTTGAGTTTGATAAACTGAATTTGCAAAATATAGGACGTAG
  CGTTTCCAAGACACCAGATGAAAGTACAAAAACTAAAGATCAGATCCTGACTTCAAGAATCAATGCAGTA
  GAAAGAGACTTGTTAGAGCCTTCTCCCGCAGACCAACTCGGGAATGGCCACAGGAGGACAGAAAGTGAAA
  TGTCAGCCAGGATCGCTAAAATGTCCTTGAGTCCCAGCAGCCCCAGGCACGAGGATCAGCTCGAGGTCAC
  CAGGGAACCGGCCAGGCGGCTCTTCCTTTTTGGAGAGGAGCCATCAAAACTCGATCAGGATGTTTTGGCC
  GCTCTTGAATGTGCAGACGTCGACCCCCATCAGTTCCCGGCCGTGCACAGATGGAAGAGTGCTGTCCTGT
  GCTACTCACCCTCGGACAGACAGAGTTGGCCCAGTCCCGCGGTGAAAGGAAGGTTCAAGTCTCAGCTGCC
  AGATCTCAGTGGCCCTCACAGCTACAGTCCGGGGAGAAACAGCGTGGCTGGAAGCAACCCCGCAAAGCCA
  GGCCTGGGCAGTCCTGGGCGCTACAGCCCCGTGCACGGGAGCCAGCTCCGCAGGATGGCGCGCCTGGCTG
  AGCTTGCCGCCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216867 representing NM_015114
  Red=Cloning site Green=Tags(s)
  
  MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLA
  RLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKP
  AEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVP
  ARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDR
  LKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGC
  RYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFAC
  KFGNADVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPLLRAEETSSPV
  IGELWSPDQTAEASHVSRYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPER
  GFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCRDKATTS
  GSNSISVRAFLDEDDMSLEEIKNRQNAARNNSPPTVGAFGHTRCSAFPLEQEADLIEAAEPGGPHSSRNG
  LCHPLNHSRTLAGKRPKAPRGEEAHLPPVSDLTVEFDKLNLQNIGRSVSKTPDESTKTKDQILTSRINAV
  ERDLLEPSPADQLGNGHRRTESEMSARIAKMSLSPSSPRHEDQLEVTREPARRLFLFGEEPSKLDQDVLA
  ALECADVDPHQFPAVHRWKSAVLCYSPSDRQSWPSPAVKGRFKSQLPDLSGPHSYSPGRNSVAGSNPAKP
  GLGSPGRYSPVHGSQLRRMARLAELAAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015114
• ORF Size: 2814 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015114.3
• RefSeq Size: 4491 bp
• RefSeq ORF: 2817 bp
• Locus ID: 23141
• UniProt ID: Q86XL3
• Cytogenetics: 12q24.33
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq, Mar 2014]