DAZAP1 (NM_170711) Human Tagged ORF Clone

CAT#: RG216522

DAZAP1 (tGFP-tagged) - Human DAZ associated protein 1 (DAZAP1), transcript variant 1

Product Images

Circular map for RG216522

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DAZAP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216522 representing NM_170711
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGCACGACCCAAG
  AGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCAC
  CAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGGTGCTGGCCAGC
  AGACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGA
  GAACACGGCCGAAGGAAGGATGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGT
  CGGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG
  GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACG
  AACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTAAACG
  AGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTT
  GTGCCCAACGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAG
  GAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCC
  GCCACCCCCACCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTC
  CCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGCCAGATCAGT
  TTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCC
  GTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGGC
  CTGGGTTCCTATTCTCCAGCCCCGCCGGGCTGCGGCCCACACTTTGTTTACAGTCTTATGGTCAGGCTGA
  GCAGTGATGTGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216522 representing NM_170711
  Red=Cloning site Green=Tags(s)
  
  MNNSGADEIGKLFVGGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTVLAS
  RPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNKIFVGGIPHNCGETELREYFKKFGVVT
  EVVMIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRV
  VPNAANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSTPPGGFPPPQGF
  PQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPATPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYG
  LGSYSPAPPGCGPHFVYSLMVRLSSDVA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170711
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170711.3
• RefSeq Size: 2274 bp
• RefSeq ORF: 1137 bp
• Locus ID: 26528
• UniProt ID: Q96EP5
• Cytogenetics: 19p13.3
• Protein Families: Stem cell - Pluripotency
• Gene Summary: In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]