AVL9 (NM_015060) Human Tagged ORF Clone

CAT#: RG216339

AVL9 (tGFP-tagged) - Human AVL9 homolog (S. cerevisiase) (AVL9)

Product Images

Circular map for RG216339

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AVL9
• Synonyms: KIAA0241
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216339 representing NM_015060
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGGCCAGGAGAGGCGGGGATGGCGTCCCCCGGGGGCCCGTACTGCACATCGTGGTGGTCGGAT
  TTCACCACAAGAAGGGCTGCCAGGTTGAATTCTCTTACCCGCCCCTGATTCCAGGAGATGGACATGACAG
  CCACACTTTACCTGAAGAATGGAAGTATTTGCCCTTCCTTGCCTTACCAGATGGCGCACACAACTACCAG
  GAAGATACTGTGTTTTTTCACTTGCCACCCAGAAATGGAAATGGAGCCACAGTATTTGGTATCTCTTGCT
  ATCGACAAATTGAAGCCAAGGCACTGAAAGTAAGGCAAGCAGATATCACCAGAGAGACTGTTCAGAAAAG
  TGTCTGTGTTCTAAGCAAGCTGCCTCTGTATGGTTTACTTCAAGCAAAACTTCAACTCATTACACATGCA
  TATTTTGAAGAGAAGGATTTTTCCCAAATTTCTATTCTAAAGGAGCTTTATGAACATATGAATAGTTCCT
  TGGGAGGTGCTTCATTAGAAGGATCCCAAGTATATCTTGGTCTGTCACCTCGAGATCTTGTCCTTCATTT
  TCGACACAAGGTCTTAATCCTATTTAAGCTAATTCTTCTTGAAAAAAAGGTTCTTTTTTATATTTCTCCA
  GTGAATAAATTGGTGGGTGCACTGATGACTGTGTTATCCCTTTTTCCAGGCATGATTGAACATGGTCTCA
  GTGACTGTTCTCAGTATAGACCCCGGAAAAGTATGTCTGAAGATGGTGGGCTTCAGGAAAGTAACCCATG
  TGCAGATGATTTTGTTTCTGCATCCACTGCTGATGTTTCACATACCAACTTGGGAACTATCAGGAAAGTC
  ATGGCAGGAAACCATGGAGAAGATGCTGCCATGAAGACTGAGGAGCCTTTGTTCCAAGTGGAAGACAGCA
  GCAAAGGGCAGGAACCCAATGATACCAATCAATATTTGAAACCTCCATCTCGCCCATCTCCAGATTCTTC
  AGAAAGTGACTGGGAAACTTTGGATCCTAGTGTCTTAGAGGACCCCAACTTGAAAGAAAGGGAACAGCTG
  GGATCAGACCAGACAAATTTGTTTCCAAAGGACTCTGTCCCCTCAGAGAGTCTTCCAATTACTGTACAAC
  CTCAAGCTAATACGGGACAGGTAGTCCTGATACCAGGGCTCATTTCGGGTTTGGAAGAGGATCAGTATGG
  CATGCCCCTGGCCATCTTCACAAAGGGATATCTGTGTTTGCCTTACATGGCATTGCAGCAGCATCATCTT
  CTCTCCGATGTCACCGTTCGGGGGTTTGTTGCTGGAGCTACTAACATCCTTTTTCGACAACAGAAACACC
  TCAGTGATGCCATTGTGGAAGTAGAAGAAGCTCTGATCCAGATCCATGATCCAGAACTCAGGAAGCTGCT
  TAACCCAACCACTGCAGACCTAAGGTTCGCAGACTACCTAGTGAGGCACGTGACTGAGAATCGGGATGAC
  GTCTTCCTAGATGGCACGGGCTGGGAGGGAGGTGACGAATGGATCCGGGCCCAGTTTGCGGTCTACATTC
  ATGCCCTGCTGGCTGCCACGCTGCAATTAGACAATGAAAAGATATTATCGGACTATGGGACAACTTTTGT
  TACAGCATGGAAGAATACTCACAACTACAGGGTGTGGAACAGCAACAAGCATCCAGCACTTGCAGAAATA
  AATCCAAACCATCCATTTCAAGGCCAATACTCAGTATCAGACATGAAGTTAAGGTTCTCACATTCTGTTC
  AGAATAGTGAACGTGGCAAAAAAATTGGAAACGTCATGGTCACAACTAGCCGGAATGTTGTACAAACAGG
  AAAAGCTGTTGGCCAGTCAGTTGGAGGAGCTTTTTCCAGTGCAAAGACAGCTATGTCTTCATGGCTTTCC
  ACTTTCACCACTTCCACCTCCCAAAGTCTCACTGAGCCACCAGATGAGAAGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216339 representing NM_015060
  Red=Cloning site Green=Tags(s)
  
  MEKARRGGDGVPRGPVLHIVVVGFHHKKGCQVEFSYPPLIPGDGHDSHTLPEEWKYLPFLALPDGAHNYQ
  EDTVFFHLPPRNGNGATVFGISCYRQIEAKALKVRQADITRETVQKSVCVLSKLPLYGLLQAKLQLITHA
  YFEEKDFSQISILKELYEHMNSSLGGASLEGSQVYLGLSPRDLVLHFRHKVLILFKLILLEKKVLFYISP
  VNKLVGALMTVLSLFPGMIEHGLSDCSQYRPRKSMSEDGGLQESNPCADDFVSASTADVSHTNLGTIRKV
  MAGNHGEDAAMKTEEPLFQVEDSSKGQEPNDTNQYLKPPSRPSPDSSESDWETLDPSVLEDPNLKEREQL
  GSDQTNLFPKDSVPSESLPITVQPQANTGQVVLIPGLISGLEEDQYGMPLAIFTKGYLCLPYMALQQHHL
  LSDVTVRGFVAGATNILFRQQKHLSDAIVEVEEALIQIHDPELRKLLNPTTADLRFADYLVRHVTENRDD
  VFLDGTGWEGGDEWIRAQFAVYIHALLAATLQLDNEKILSDYGTTFVTAWKNTHNYRVWNSNKHPALAEI
  NPNHPFQGQYSVSDMKLRFSHSVQNSERGKKIGNVMVTTSRNVVQTGKAVGQSVGGAFSSAKTAMSSWLS
  TFTTSTSQSLTEPPDEKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015060
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015060.1, NP_055875.1
• RefSeq Size: 2353 bp
• RefSeq ORF: 1947 bp
• Locus ID: 23080
• UniProt ID: Q8NBF6
• Cytogenetics: 7p14.3
• Gene Summary: Functions in cell migration.[UniProtKB/Swiss-Prot Function]