SLC27A5 (NM_012254) Human Tagged ORF Clone

CAT#: RG215758

SLC27A5 (tGFP-tagged) - Human solute carrier family 27 (fatty acid transporter), member 5 (SLC27A5)

Product Images

Circular map for RG215758

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC27A5
• Synonyms: ACSB; ACSVL6; BACS; BAL; FACVL3; FATP-5; FATP5; VLACSR; VLCS-H2; VLCSH2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215758 representing NM_012254
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTCAGGCAACAGTTGGCCTTGCTGCTGCTGCTGCTGCTCCTGCTCTGGGGCCTGGGGCAGCCAG
  TGTGGCCAGTCGCTGTGGCCTTGACCCTGCGCTGGCTCCTGGGGGATCCCACATGTTGCGTGCTACTTGG
  GCTGGCCATGTTAGCACGGCCCTGGCTCGGCCCCTGGGTGCCCCATGGGCTGAGCCTGGCAGCTGCGGCC
  CTGGCACTAACCCTCCTGCCAGCACGGCTGCCCCCAGGACTACGCTGGCTGCCGGCTGATGTGATCTTCT
  TGGCCAAGATCCTCCACCTGGGCCTGAAGATCAGGGGATGCTTGAGCCGGCAGCCGCCTGACACCTTTGT
  AGATGCCTTCGAGCGGCGAGCACGAGCGCAGCCTGGCAGGGCACTCTTGGTGTGGACGGGGCCTGGGGCC
  GGCTCAGTCACCTTTGGTGAGCTGGATGCCCGGGCCTGCCAGGCGGCATGGGCCCTGAAGGCTGAGCTGG
  GTGACCCTGCGAGCCTGTGTGCCGGGGAGCCTACTGCCCTCCTTGTGCTGGCTTCCCAGGCCGTTCCAGC
  CCTGTGTATGTGGCTGGGGCTGGCCAAGCTGGGCTGCCCAACAGCCTGGATCAACCCGCATGGCCGGGGG
  ATGCCCCTGGCGCACTCTGTGCTGAGCTCTGGGGCCCGGGTGCTGGTGGTGGACCCAGACCTCCGGGAGA
  GCCTGGAGGAGATCCTTCCCAAGCTGCAGGCTGAGAACATCCGCTGCTTCTACCTCAGCCATACCTCCCC
  TACACCAGGGGTGGGGGCTCTGGGGGCTGCCCTGGATGCAGCGCCCTCCCACCCAGTGCCTGCTGACCTG
  CGTGCTGGGATCACATGGAGAAGCCCTGCCCTCTTCATCTATACCTCGGGGACCACTGGCCTCCCGAAGC
  CAGCCATCCTCACGCATGAGCGGGTACTGCAGATGAGCAAGATGCTGTCCTTATCTGGGGCCACAGCTGA
  TGATGTGGTTTACACGGTCCTGCCTCTGTACCACGTGATGGGACTTGTCGTTGGGATCCTCGGCTGCTTA
  GATCTCGGAGCCACCTGTGTTCTGGCCCCCAAGTTCTCTACTTCCTGCTTCTGGGATGACTGTCGGCAGC
  ATGGCGTGACAGTGATCCTGTATGTGGGCGAGCTCCTGCGGTACTTGTGTAACATTCCCCAGCAACCAGA
  GGACCGGACACATACAGTCCGCCTGGCAATGGGCAATGGACTACGGGCTGATGTGTGGGAGACCTTCCAG
  CAGCGCTTCGGTCCTATTCGGATCTGGGAAGTCTACGGCTCCACAGAAGGCAACATGGGCTTAGTCAACT
  ATGTGGGGCGCTGCGGGGCCCTGGGCAAGATGAGCTGCCTCCTCCGAATGCTGTCCCCCTTTGAGCTGGT
  GCAGTTCGACATGGAGGCGGCGGAGCCTGTGAGGGACAATCAGGGCTTCTGCATCCCTGTAGGGCTAGGG
  GAGCCGGGGCTGCTGCTGACCAAGGTGGTAAGCCAGCAACCCTTCGTGGGCTACCGCGGCCCCCGAGAGC
  TGTCGGAACGGAAGCTGGTGCGCAACGTGCGGCAATCGGGCGACGTTTACTACAACACCGGGGACGTACT
  GGCCATGGACCGCGAAGGCTTCCTCTACTTCCGCGACCGCCTCGGGGACACCTTCCGATGGAAGGGCGAG
  AACGTGTCCACGCACGAGGTGGAGGGCGTGTTGTCGCAGGTGGACTTCTTGCAACAGGTTAACGTGTATG
  GCGTGTGCGTGCCAGGTTGTGAGGGTAAGGTGGGCATGGCTGCTGTGCAGCTAGCCCCCGGCCAGACTTT
  CGACGGGGAGAAGTTGTACCAGCACGTTCGCGCTTGGCTCCCTGCCTACGCTACCCCCCATTTCATCCGC
  ATCCAGGACGCCATGGAGGTCACCAGCACGTTCAAACTGATGAAGACCCGGTTGGTGCGTGAGGGCTTCA
  ATGTGGGGATCGTGGTTGACCCTCTGTTTGTACTGGACAACCGGGCCCAGTCCTTCCGGCCCCTGACGGC
  AGAAATGTACCAGGCTGTGTGTGAGGGAACCTGGAGGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215758 representing NM_012254
  Red=Cloning site Green=Tags(s)
  
  MGVRQQLALLLLLLLLLWGLGQPVWPVAVALTLRWLLGDPTCCVLLGLAMLARPWLGPWVPHGLSLAAAA
  LALTLLPARLPPGLRWLPADVIFLAKILHLGLKIRGCLSRQPPDTFVDAFERRARAQPGRALLVWTGPGA
  GSVTFGELDARACQAAWALKAELGDPASLCAGEPTALLVLASQAVPALCMWLGLAKLGCPTAWINPHGRG
  MPLAHSVLSSGARVLVVDPDLRESLEEILPKLQAENIRCFYLSHTSPTPGVGALGAALDAAPSHPVPADL
  RAGITWRSPALFIYTSGTTGLPKPAILTHERVLQMSKMLSLSGATADDVVYTVLPLYHVMGLVVGILGCL
  DLGATCVLAPKFSTSCFWDDCRQHGVTVILYVGELLRYLCNIPQQPEDRTHTVRLAMGNGLRADVWETFQ
  QRFGPIRIWEVYGSTEGNMGLVNYVGRCGALGKMSCLLRMLSPFELVQFDMEAAEPVRDNQGFCIPVGLG
  EPGLLLTKVVSQQPFVGYRGPRELSERKLVRNVRQSGDVYYNTGDVLAMDREGFLYFRDRLGDTFRWKGE
  NVSTHEVEGVLSQVDFLQQVNVYGVCVPGCEGKVGMAAVQLAPGQTFDGEKLYQHVRAWLPAYATPHFIR
  IQDAMEVTSTFKLMKTRLVREGFNVGIVVDPLFVLDNRAQSFRPLTAEMYQAVCEGTWRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012254
• ORF Size: 2070 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012254.3
• RefSeq Size: 2378 bp
• RefSeq ORF: 2073 bp
• Locus ID: 10998
• UniProt ID: Q9Y2P5
• Cytogenetics: 19q13.43
• Domains: AMP-binding
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
• Gene Summary: The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog. [provided by RefSeq, Jul 2008]